Due to sturdy electrical fields at the floor, the absorption and scattering of electromagnetic radiation by noble metallic nanoparticles are strongly enhanced. These distinctive properties present the potential of designing novel optically energetic reagents for simultaneous molecular imaging and photothermal most cancers therapy. It is fascinating to make use of brokers which are energetic in the near-infrared (NIR) region of the radiation spectrum to attenuate the gentle extinction by intrinsic chromophores in native tissue. Gold nanorods with appropriate side ratios (size divided by width) can soak up and scatter strongly in the NIR region (650-900 nm).
In the current work, we offer an in vitro demonstration of gold nanorods as novel distinction brokers for each molecular imaging and photothermal most cancers therapy. Nanorods are synthesized and conjugated to anti-epidermal progress issue receptor (anti-EGFR) monoclonal antibodies and incubated in cell cultures with a nonmalignant epithelial cell line (HaCat) and two malignant oral epithelial cell traces (HOC 313 clone 8 and HSC 3). The anti-EGFR antibody-conjugated nanorods bind particularly to the floor of the malignant-type cells with a a lot increased affinity attributable to the overexpressed EGFR on the cytoplasmic membrane of the malignant cells.
As a results of the strongly scattered crimson gentle from gold nanorods in darkish discipline, noticed using a laboratory microscope, the malignant cells are clearly visualized and recognized from the nonmalignant cells. It is discovered that, after publicity to steady crimson laser at 800 nm, malignant cells require about half the laser power to be photothermally destroyed than the nonmalignant cells. Thus, each environment friendly most cancers cell diagnostics and selective photothermal therapy are realized at the identical time.
Production of monoclonal antibodies to group A erythrocytes, HLA and different human cell floor antigens-new instruments for genetic evaluation.
Antibody-secreting hybrid cells have been derived from a fusion between mouse myeloma cells and spleen cells from a mouse immunized with membrane from human tonsil lymphocyte preparations. Hybrids secreting antibodies to cell floor antigens have been detected by assaying tradition supernatants for antibody binding to human tonsil cells. Six completely different antibodies (referred to as W6/1, /28, /32, /34, /45 and /46 have been analyzed.
These have been both towards antigens of huge tissue distribution (W6/32, /34, and /46) or primarily on erythrocytes (W6/1 and W6/28). One of the anti-erythrocyte antibodies (W6/1) detected a polymorphic antigen, since blood group A1 and A2 erythrocytes have been labeled whereas B and O weren’t.
Antibodies W6/34, /45 and /46 have been all towards antigens which have been mapped to the quick arm of chromosome 11 by segregation evaluation of mouse-human hybrids. Immunoprecipitation research recommend that W6/45 antigen could also be a protein of 16,000 dalton, obvious molecular weight, whereas W6/34 and /46 antigens couldn’t be detected by this method.
Antibody W6/32 is towards a determinant widespread to most, if not all, of the 43,000 dalton molecular weight chains of HLA-A, B and C antigens. This was established by somatic cell genetic strategies and by immunoprecipitation evaluation. Tonsil leucocytes certain 370,000 W6/32 antibody molecules per cell at saturation. The hybrid myelomas W6/32 and W6/34 have been cloned, and each secrete an IgG2 antibody.
W6/32 cells have been grown in mice, and the serum of the tumor-bearing animals contained higher than 10 mg/ml of monoclonal antibody. The experiments established the usefulness of the bybrid myeloma approach in making ready monospecific antibodies towards human cell floor antigens. In explicit, this research highlights the potentialities not solely of acquiring reagents for somatic cell genetics, but additionally of acquiring mouse antibodies detecting human antigenic polymorphisms.
Sulforhodamine B colorimetric assay for cytotoxicity screening.
The sulforhodamine B (SRB) assay is used for cell density dedication, based mostly on the measurement of mobile protein content material. The technique described right here has been optimized for the toxicity screening of compounds to adherent cells in a 96-well format.
After an incubation interval, cell monolayers are mounted with 10% (wt/vol) trichloroacetic acid and stained for 30 min, after which the extra dye is eliminated by washing repeatedly with 1% (vol/vol) acetic acid. The protein-bound dye is dissolved in 10 mM Tris base resolution for OD dedication at 510 nm using a microplate reader.
The outcomes are linear over a 20-fold vary of cell numbers and the sensitivity is corresponding to these of fluorometric strategies. The technique not solely permits a lot of samples to be examined inside just a few days, but additionally requires solely easy gear and cheap reagents. The SRB assay is subsequently an environment friendly and extremely cost-effective technique for screening.
Gene splicing and mutagenesis by PCR-driven overlap extension.
Extension of overlapping gene segments by PCR is an easy, versatile approach for site-directed mutagenesis and gene splicing. Initial PCRs generate overlapping gene segments which are then used as template DNA for an additional PCR to create a full-length product. Internal primers generate overlapping, complementary 3′ ends on the intermediate segments and introduce nucleotide substitutions, insertions or deletions for site-directed mutagenesis, or for gene splicing, encode the nucleotides discovered at the junction of adjoining gene segments.
Oxidase Strip Containing Reagent |
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| MIC6738 | Scientific Laboratory Supplies | EACH | 43.09 EUR |
Mouse IgG Blocking Reagent |
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| 30R-AH071 | Fitzgerald | 20 mg | 219 EUR |
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Description: Mouse IgG (H + L) Blocking Reagent |
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Mouse IgG Blocking Reagent |
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| MBS537317-10x10mg | MyBiosource | 10x10mg | 1365 EUR |
Mouse IgG Blocking Reagent |
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| MBS537317-2x10mg | MyBiosource | 2x10mg | 335 EUR |
QUANTUM SIMPLY CELLULAR MOUSE IgG Reagent |
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| GWB-Q00327 | GenWay Biotech | 20 TESTS | Ask for price |
QUANTUM SIMPLY CELLULAR MOUSE IgG Reagent |
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| GWB-Q00328 | GenWay Biotech | 100 TESTS | Ask for price |
QUANTUM SIMPLY CELLULAR MOUSE IgG Reagent |
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| GWB-Q00329 | GenWay Biotech | 280 TESTS | Ask for price |
EP Reagent Biuret Reagent - 100ML |
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| 1011601 | Scientific Laboratory Supplies | 1L | 59.4 EUR |
PEROXIDE BLOCKING REAGENT Reagent |
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| GWB-Q00308 | GenWay Biotech | 50 ml | Ask for price |
EP Reagent Iodoplatinate Reagent - 200ML |
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| 1046300 | Scientific Laboratory Supplies | 200ML | 502.2 EUR |
EP Reagent Molybdovanadic Reagent - 100ML |
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| 1056700 | Scientific Laboratory Supplies | 100ML | 59.4 EUR |
EP Reagent Methoxyphenylacetic Reagent - 100ML |
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| 1053601 | Scientific Laboratory Supplies | 100ML | 490.05 EUR |
EP Reagent Phosphomolybdotungstic Reagent - 100ML |
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| 1065000 | Scientific Laboratory Supplies | 100ML | 226.8 EUR |
Enhanced Chemiluminescent Reagent Kit (Anti-Rat IgG) |
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| EK1004 | BosterBio | 1 kit (1000 cm2) | 182.4 EUR |
AVIDIN/BIOTIN BLOCKING REAGENT Reagent |
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| GWB-Q00289 | GenWay Biotech | 30 ml | Ask for price |
Gordon-McLeod Reagent (Oxidase reagent) |
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| R026-100ML | EWC Diagnostics | 1 unit | 7.76 EUR |
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Description: Gordon-McLeod Reagent (Oxidase reagent) |
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Enhanced Chemiluminescent Reagent Kit (Anti-Goat IgG) |
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| EK1003 | BosterBio | 1 kit (1000 cm2) | 182.4 EUR |
Oxidase Reagent (Gordon-Macleod Reagent) |
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| 76207 | Sisco Laboratories | 100 ml | 3.38 EUR |
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Description: Part B |
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Enhanced Chemiluminescent Reagent Kit (Anti-Mouse IgG) |
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| EK1001 | BosterBio | 1 kit (1000 cm2) | 182.4 EUR |
Enhanced Chemiluminescent Reagent Kit (Anti-Rabbit IgG) |
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| EK1002 | BosterBio | 1 kit (1000 cm2) | 182.4 EUR |
EP Reagent Sulfomolybdic Reagent R3 - 1L |
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| 1086500 | Scientific Laboratory Supplies | 1L | 398.25 EUR |
TRIpure Reagent (same as Trizol Reagent) |
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| MBS356001-INQUIRE | MyBiosource | INQUIRE | Ask for price |
STREPTAVIDIN Reagent |
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| GWB-Q00241 | GenWay Biotech | 5 mg | Ask for price |
Total RNA Isolation Reagent (Reagent Based) |
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| MBS402052-100mL | MyBiosource | 100mL | 235 EUR |
Total RNA Isolation Reagent (Reagent Based) |
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| MBS402052-4Reactions | MyBiosource | 4Reactions | 165 EUR |
STREPTAVIDIN:HRP Reagent |
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| GWB-Q00198 | GenWay Biotech | 1 mg | Ask for price |
STREPTAVIDIN:HRP Reagent |
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| GWB-Q00243 | GenWay Biotech | 1 ml | Ask for price |
STREPTAVIDIN:APC Reagent |
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| GWB-Q00332 | GenWay Biotech | 100 TESTS | Ask for price |
STREPTAVIDIN:RPE Reagent |
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| GWB-Q00336 | GenWay Biotech | 100 TESTS | Ask for price |
STREPTAVIDIN:RPE Reagent |
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| GWB-Q00337 | GenWay Biotech | 500 TESTS | Ask for price |
Genomic DNA Isolation Reagent (Reagent Based) |
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| MBS402050-100mL | MyBiosource | 100mL | 255 EUR |
Genomic DNA Isolation Reagent (Reagent Based) |
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| MBS402050-4Reactions | MyBiosource | 4Reactions | 185 EUR |
STREPTAVIDIN:FITC Reagent |
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| GWB-Q01614 | GenWay Biotech | 1 mg | Ask for price |
STREPTAVIDIN:TRITC Reagent |
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| GWB-Q01615 | GenWay Biotech | 1 mg | Ask for price |
COMBI IC Reagent: IgG Negative Control (FITC) and IgG Negative Control (PE) |
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| MBS570400-1mL | MyBiosource | 1mL | 370 EUR |
COMBI IC Reagent: IgG Negative Control (FITC) and IgG Negative Control (PE) |
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| MBS570400-5x1mL | MyBiosource | 5x1mL | 1525 EUR |
Plant Total RNA Isolation Reagent (Reagent Based) |
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| MBS402053-100mL | MyBiosource | 100mL | 245 EUR |
Plant Total RNA Isolation Reagent (Reagent Based) |
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| MBS402053-4Reactions | MyBiosource | 4Reactions | 165 EUR |
Plant Genomic DNA Isolation Reagent (Reagent Based) |
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| MBS402051-100mL | MyBiosource | 100mL | 245 EUR |
Plant Genomic DNA Isolation Reagent (Reagent Based) |
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| MBS402051-4Reactions | MyBiosource | 4Reactions | 165 EUR |
Western Blotting Rat IgG DAB Chromogenic Reagent Kit (Yellow) |
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| SA2023 | BosterBio | 1 kit(1200 cm2) | 169.2 EUR |
Western Blotting Mouse IgG DAB Chromogenic Reagent Kit (Blue) |
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| SA2024 | BosterBio | 1 kit(1200 cm2) | 169.2 EUR |
N.N.C.D.-reagent |
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| N632003 | Toronto Research Chemicals | 1g | 1200 EUR |
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Description: 6035-19-4 |
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STREPTAVIDIN: FITC Reagent |
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| GWB-Q00335 | GenWay Biotech | 1 mg | Ask for price |
Western Blotting Goat IgG DAB Chromogenic Reagent Kit (Yellow) |
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| SA2021 | BosterBio | 1 kit(1200 cm2) | 169.2 EUR |
Western Blotting Rabbit IgG DAB Chromogenic Reagent Kit (Blue) |
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| SA2025 | BosterBio | 1 kit(1200 cm2) | 169.2 EUR |
BOP reagent |
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| A7015-100000 | ApexBio | 100 g | 104 EUR |
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Description: Amino Acids & Building Blocks|Peptide Coupling Reagents |
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BOP reagent |
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| A7015-25000 | ApexBio | 25 g | 44 EUR |
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Description: Amino Acids & Building Blocks|Peptide Coupling Reagents |
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BOP reagent |
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| 5-02141 | CHI Scientific | 25g | 32.76 EUR |
BOP reagent |
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| 5-02142 | CHI Scientific | 100g | 98.28 EUR |
MTS Reagent |
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| 2808-1000 | Biovision | each | 1188 EUR |
MTS Reagent |
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| 2808-250 | Biovision | each | 438 EUR |
MTT Reagent |
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| 2809-1G | Biovision | each | 216 EUR |
MTT Reagent |
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| 2809-5G | Biovision | each | 652.8 EUR |
BOP Reagent |
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| MBS406584-100g | MyBiosource | 100g | 330 EUR |
BOP Reagent |
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| MBS406584-25g | MyBiosource | 25g | 200 EUR |
BOP Reagent |
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| MBS406584-5g | MyBiosource | 5g | 135 EUR |
BOP Reagent |
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| MBS406584-5x25g | MyBiosource | 5x25g | 855 EUR |
BOP Reagent |
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| MBS406584-5x5g | MyBiosource | 5x5g | 565 EUR |
TDA Reagent |
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| R036-10ML | EWC Diagnostics | 1 unit | 5.22 EUR |
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Description: TDA Reagent |
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PYR Reagent |
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| R043-10ML | EWC Diagnostics | 1 unit | 12.02 EUR |
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Description: PYR Reagent |
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Reagent 2R2 |
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| ZV-3017-05R2-15 | Zivak Technologies | 30 mL | Ask for price |
Reagent 3R3 |
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| ZV-3017-05R3-15 | Zivak Technologies | 50 mL | Ask for price |
Western Blotting Mouse IgG DAB Chromogenic Reagent Kit (Yellow) |
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| SA2020 | BosterBio | 1 kit(1200 cm2) | 169.2 EUR |
STREPTAVIDIN:Alk. Phos. Reagent |
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| GWB-Q00197 | GenWay Biotech | 1 mg | Ask for price |
STREPTAVIDIN:Alk. Phos. Reagent |
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| GWB-Q00242 | GenWay Biotech | 1 ml | Ask for price |
Bial’s Reagent |
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| 83710 | Sisco Laboratories | 250 ml | 4.65 EUR |
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Description: Part B |
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Reagent pack |
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| A11-100 | Awareness Technology | 1 unit | 123 EUR |
pNPP Reagent |
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| GWB-Q00238 | GenWay Biotech | 100 ml | Ask for price |
pNPP Reagent |
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| GWB-Q00239 | GenWay Biotech | 500 ml | Ask for price |
HCTU Reagent |
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| MBS406580-100g | MyBiosource | 100g | 260 EUR |
HCTU Reagent |
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| MBS406580-25g | MyBiosource | 25g | 180 EUR |
HCTU Reagent |
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| MBS406580-5g | MyBiosource | 5g | 135 EUR |
HCTU Reagent |
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| MBS406580-5x25g | MyBiosource | 5x25g | 750 EUR |
HCTU Reagent |
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| MBS406580-5x5g | MyBiosource | 5x5g | 565 EUR |
COMU Reagent |
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| MBS406581-100g | MyBiosource | 100g | 730 EUR |
COMU Reagent |
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| MBS406581-25g | MyBiosource | 25g | 305 EUR |
COMU Reagent |
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| MBS406581-5g | MyBiosource | 5g | 160 EUR |
COMU Reagent |
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| MBS406581-5x25g | MyBiosource | 5x25g | 1310 EUR |
COMU Reagent |
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| MBS406581-5x5g | MyBiosource | 5x5g | 660 EUR |
HBTU Reagent |
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| MBS406583-100g | MyBiosource | 100g | 260 EUR |
HBTU Reagent |
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| MBS406583-25g | MyBiosource | 25g | 180 EUR |
HBTU Reagent |
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| MBS406583-5g | MyBiosource | 5g | 135 EUR |
HBTU Reagent |
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| MBS406583-5x25g | MyBiosource | 5x25g | 750 EUR |
HBTU Reagent |
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| MBS406583-5x5g | MyBiosource | 5x5g | 565 EUR |
TBTU Reagent |
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| MBS406586-100g | MyBiosource | 100g | 355 EUR |
TBTU Reagent |
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| MBS406586-25g | MyBiosource | 25g | 225 EUR |
TBTU Reagent |
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| MBS406586-5g | MyBiosource | 5g | 150 EUR |
TBTU Reagent |
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| MBS406586-5x25g | MyBiosource | 5x25g | 970 EUR |
TBTU Reagent |
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| MBS406586-5x5g | MyBiosource | 5x5g | 635 EUR |
TCTU Reagent |
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| MBS406596-100g | MyBiosource | 100g | 260 EUR |
TCTU Reagent |
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| MBS406596-25g | MyBiosource | 25g | 180 EUR |
TCTU Reagent |
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| MBS406596-5g | MyBiosource | 5g | 135 EUR |
TCTU Reagent |
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| MBS406596-5x25g | MyBiosource | 5x25g | 750 EUR |
TCTU Reagent |
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| MBS406596-5x5g | MyBiosource | 5x5g | 565 EUR |
DAPI Reagent |
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| MBS2567122-100Tests | MyBiosource | 100Tests | 85 EUR |
DAPI Reagent |
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| MBS2567122-200Tests | MyBiosource | 200Tests | 95 EUR |
DAPI Reagent |
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| MBS2567122-500Tests | MyBiosource | 500Tests | 115 EUR |
DAPI Reagent |
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| MBS2567122-50Tests | MyBiosource | 50Tests | 80 EUR |
DAPI Reagent |
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| MBS2567122-5x500Tests | MyBiosource | 5x500Tests | 510 EUR |
DAPI Reagent |
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| MBS2563514-10mL | MyBiosource | 10mL | 100 EUR |
DAPI Reagent |
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| MBS2563514-50mL | MyBiosource | 50mL | 150 EUR |
DAPI Reagent |
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| MBS2563514-5mL | MyBiosource | 5mL | 90 EUR |
DAPI Reagent |
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| MBS2563514-5x50mL | MyBiosource | 5x50mL | 640 EUR |
Western Blotting Rabbit IgG DAB Chromogenic Reagent Kit (Yellow) |
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| SA2022 | BosterBio | 1 kit(1200 cm2) | 169.2 EUR |
STREPTAVIDIN:RPE-Cy5 Reagent |
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| GWB-Q00338 | GenWay Biotech | 0.1 mg | Ask for price |
AA Reagent-1 |
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| ZV-3002-02R1-10 | Zivak Technologies | 1 x 30 mL /200 Tests | Ask for price |
AA Reagent-2 |
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| ZV-3002-02R2-10 | Zivak Technologies | 1 x 50 mL/200 Tests | Ask for price |
AA Reagent-3 |
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| ZV-3002-02R3-10 | Zivak Technologies | 1 x 50 mL/200 Tests | Ask for price |
AA Reagent-4 |
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| ZV-3002-02R4-10 | Zivak Technologies | 1 x 50 mL/200 Tests | Ask for price |
Gibbs reagent |
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| 573630 | MedKoo Biosciences | 1.0g | 295 EUR |
Overlapping strands of those intermediate merchandise hybridize at this 3′ region in a subsequent PCR and are prolonged to generate the full-length product amplified by flanking primers that may embrace restriction enzyme websites for inserting the product into an expression vector for cloning functions. The extremely environment friendly technology of mutant or chimeric genes by this technique can simply be completed with normal laboratory reagents in roughly 1 week.