Due to sturdy electrical fields at the floor, the absorption and scattering of electromagnetic radiation by noble metallic nanoparticles are strongly enhanced. These distinctive properties present the potential of designing novel optically energetic reagents for simultaneous molecular imaging and photothermal most cancers therapy. It is fascinating to make use of brokers which are energetic in the near-infrared (NIR) region of the radiation spectrum to attenuate the gentle extinction by intrinsic chromophores in native tissue. Gold nanorods with appropriate side ratios (size divided by width) can soak up and scatter strongly in the NIR region (650-900 nm).
In the current work, we offer an in vitro demonstration of gold nanorods as novel distinction brokers for each molecular imaging and photothermal most cancers therapy. Nanorods are synthesized and conjugated to anti-epidermal progress issue receptor (anti-EGFR) monoclonal antibodies and incubated in cell cultures with a nonmalignant epithelial cell line (HaCat) and two malignant oral epithelial cell traces (HOC 313 clone 8 and HSC 3). The anti-EGFR antibody-conjugated nanorods bind particularly to the floor of the malignant-type cells with a a lot increased affinity attributable to the overexpressed EGFR on the cytoplasmic membrane of the malignant cells.
As a results of the strongly scattered crimson gentle from gold nanorods in darkish discipline, noticed using a laboratory microscope, the malignant cells are clearly visualized and recognized from the nonmalignant cells. It is discovered that, after publicity to steady crimson laser at 800 nm, malignant cells require about half the laser power to be photothermally destroyed than the nonmalignant cells. Thus, each environment friendly most cancers cell diagnostics and selective photothermal therapy are realized at the identical time.
Production of monoclonal antibodies to group A erythrocytes, HLA and different human cell floor antigens-new instruments for genetic evaluation.
Antibody-secreting hybrid cells have been derived from a fusion between mouse myeloma cells and spleen cells from a mouse immunized with membrane from human tonsil lymphocyte preparations. Hybrids secreting antibodies to cell floor antigens have been detected by assaying tradition supernatants for antibody binding to human tonsil cells. Six completely different antibodies (referred to as W6/1, /28, /32, /34, /45 and /46 have been analyzed.
These have been both towards antigens of huge tissue distribution (W6/32, /34, and /46) or primarily on erythrocytes (W6/1 and W6/28). One of the anti-erythrocyte antibodies (W6/1) detected a polymorphic antigen, since blood group A1 and A2 erythrocytes have been labeled whereas B and O weren’t.
Antibodies W6/34, /45 and /46 have been all towards antigens which have been mapped to the quick arm of chromosome 11 by segregation evaluation of mouse-human hybrids. Immunoprecipitation research recommend that W6/45 antigen could also be a protein of 16,000 dalton, obvious molecular weight, whereas W6/34 and /46 antigens couldn’t be detected by this method.
Antibody W6/32 is towards a determinant widespread to most, if not all, of the 43,000 dalton molecular weight chains of HLA-A, B and C antigens. This was established by somatic cell genetic strategies and by immunoprecipitation evaluation. Tonsil leucocytes certain 370,000 W6/32 antibody molecules per cell at saturation. The hybrid myelomas W6/32 and W6/34 have been cloned, and each secrete an IgG2 antibody.
W6/32 cells have been grown in mice, and the serum of the tumor-bearing animals contained higher than 10 mg/ml of monoclonal antibody. The experiments established the usefulness of the bybrid myeloma approach in making ready monospecific antibodies towards human cell floor antigens. In explicit, this research highlights the potentialities not solely of acquiring reagents for somatic cell genetics, but additionally of acquiring mouse antibodies detecting human antigenic polymorphisms.
Sulforhodamine B colorimetric assay for cytotoxicity screening.
The sulforhodamine B (SRB) assay is used for cell density dedication, based mostly on the measurement of mobile protein content material. The technique described right here has been optimized for the toxicity screening of compounds to adherent cells in a 96-well format.
After an incubation interval, cell monolayers are mounted with 10% (wt/vol) trichloroacetic acid and stained for 30 min, after which the extra dye is eliminated by washing repeatedly with 1% (vol/vol) acetic acid. The protein-bound dye is dissolved in 10 mM Tris base resolution for OD dedication at 510 nm using a microplate reader.
The outcomes are linear over a 20-fold vary of cell numbers and the sensitivity is corresponding to these of fluorometric strategies. The technique not solely permits a lot of samples to be examined inside just a few days, but additionally requires solely easy gear and cheap reagents. The SRB assay is subsequently an environment friendly and extremely cost-effective technique for screening.
Gene splicing and mutagenesis by PCR-driven overlap extension.
Extension of overlapping gene segments by PCR is an easy, versatile approach for site-directed mutagenesis and gene splicing. Initial PCRs generate overlapping gene segments which are then used as template DNA for an additional PCR to create a full-length product. Internal primers generate overlapping, complementary 3′ ends on the intermediate segments and introduce nucleotide substitutions, insertions or deletions for site-directed mutagenesis, or for gene splicing, encode the nucleotides discovered at the junction of adjoining gene segments.
Human rheumatoid factor (RF) antibody (IgG) ELISA kit |
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| CSB-EQ027571HU-24T | Cusabio | 1 plate of 24 wells | 198 EUR |
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Description: Qualitativeindirect ELISA kit for measuring Human rheumatoid factor (RF) antibody (IgG) in samples from serum, plasma. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price. |
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Human rheumatoid factor (RF) antibody (IgG) ELISA kit |
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| 1-CSB-EQ027571HU | Cusabio |
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Description: Qualitativeindirect ELISA kit for measuring Human rheumatoid factor (RF) antibody (IgG) in samples from serum, plasma. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits. |
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RF Adsorbent |
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| 85R-1007 | Fitzgerald | 1 ml | 163.2 EUR |
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Description: Rheumatoid Factor adsorbent for use in ELISA IgM assays |
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RF 9 |
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| B7463-1 | ApexBio | 1 mg | 254.4 EUR |
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Description: Ki: 58 nM for NPFF1; 75 nM for NPFF2RF 9 is a neuropeptide FF receptors antagonist. |
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RF 9 |
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| B7463-5 | ApexBio | 5 mg | 471.6 EUR |
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Description: Ki: 58 nM for NPFF1; 75 nM for NPFF2RF 9 is a neuropeptide FF receptors antagonist. |
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RF 9 |
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| B7463-5.1 | ApexBio | 10 mM (in 1mL DMSO) | 498 EUR |
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Description: Ki: 58 nM for NPFF1; 75 nM for NPFF2RF 9 is a neuropeptide FF receptors antagonist. |
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Ginsenoside Rf |
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| N1917-20 | ApexBio | 20 mg | 408 EUR |
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Description: Extracted from Panax ginseng C. A. Mey.;Store the product in sealed,cool and dry condition |
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Ginsenoside Rf |
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| HY-N0601 | MedChemExpress | 10mg | 340.8 EUR |
Ginsenoside Rf |
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| TG027172 | ChemNorm | 10mg | Ask for price |
Enhanced Chemiluminescent Reagent Kit (Anti-Mouse IgG) |
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| EK1001 | BosterBio | 1 kit (1000 cm2) | 182.4 EUR |
Enhanced Chemiluminescent Reagent Kit (Anti-Rabbit IgG) |
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| EK1002 | BosterBio | 1 kit (1000 cm2) | 182.4 EUR |
Enhanced Chemiluminescent Reagent Kit (Anti-Goat IgG) |
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| EK1003 | BosterBio | 1 kit (1000 cm2) | 182.4 EUR |
Enhanced Chemiluminescent Reagent Kit (Anti-Rat IgG) |
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| EK1004 | BosterBio | 1 kit (1000 cm2) | 182.4 EUR |
Antho-Rf amide |
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| 5-00705 | CHI Scientific | 4 x 5mg | Ask for price |
RF-amide, Chicken |
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| 5-01876 | CHI Scientific | 4 x 5mg | Ask for price |
RF-amide Peptide |
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| 20-abx265855 | Abbexa |
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EP Reagent Biuret Reagent |
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| 1011601 | Scientific Laboratory Supplies | 1L | 42.18 EUR |
EP Reagent Iodoplatinate Reagent |
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| 1046300 | Scientific Laboratory Supplies | 200ML | 360.24 EUR |
EP Reagent Methoxyphenylacetic Reagent |
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| 1053601 | Scientific Laboratory Supplies | 100ML | 354.54 EUR |
EP Reagent Molybdovanadic Reagent |
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| 1056700 | Scientific Laboratory Supplies | 100ML | 42.18 EUR |
EP Reagent Phosphomolybdotungstic Reagent |
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| 1065000 | Scientific Laboratory Supplies | 100ML | 161.88 EUR |
Human Rheumatoid Factors IgG (RF) ELISA Kit, 96 tests, Semi-Quantitative |
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| 3000-110-RFG | Alpha Diagnostics | 1 kit | 708 EUR |
Human IgG/RF-adsorbent (Adsorb 50 serum samples of 100 ul) |
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| RFAD11-50 | Alpha Diagnostics | 50 tests | 196.8 EUR |
Human IgG/RF-adsorbent (Adsorb 50 serum samples of 100 ul) |
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| RFAD11-500 | Alpha Diagnostics | 500 tests | 634.8 EUR |
RF-amide peptide, Drosophila |
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| 5-01877 | CHI Scientific | 4 x 5mg | Ask for price |
RF-amide peptide, Drosophila |
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| 20-abx266247 | Abbexa |
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Porcine RF ELISA Kit |
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| EPR0011 | Abclonal | 96Tests | 625.2 EUR |
Rabbit RF ELISA Kit |
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| ERTR0011 | Abclonal | 96Tests | 625.2 EUR |
Rat RF ELISA Kit |
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| ERR0011 | Abclonal | 96Tests | 625.2 EUR |
Goat RF ELISA Kit |
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| EGTR0011 | Abclonal | 96Tests | 625.2 EUR |
Monkey RF ELISA Kit |
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| EMKR0011 | Abclonal | 96Tests | 625.2 EUR |
Mouse RF ELISA Kit |
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| EMR0011 | Abclonal | 96Tests | 625.2 EUR |
Human RF ELISA Kit |
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| EHR0011 | Abclonal | 96Tests | 625.2 EUR |
Bovine RF ELISA Kit |
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| EBR0011 | Abclonal | 96Tests | 625.2 EUR |
Chicken RF ELISA Kit |
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| ECKR0011 | Abclonal | 96Tests | 625.2 EUR |
Anserini RF ELISA Kit |
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| EAR0011 | Abclonal | 96Tests | 625.2 EUR |
Canine RF ELISA Kit |
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| ECR0011 | Abclonal | 96Tests | 625.2 EUR |
Sheep RF ELISA Kit |
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| ESR0011 | Abclonal | 96Tests | 625.2 EUR |
EP Reagent Sulfomolybdic Reagent R3 |
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| 1086500 | Scientific Laboratory Supplies | 1L | 287.28 EUR |
Bovine Immunoglobulin G (IgG) ELISA Kit |
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| DLR-IgG-b-48T | DL Develop | 48T | 547.2 EUR |
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Description: A competitive inhibition quantitative ELISA assay kit for detection of Bovine Immunoglobulin G (IgG) in samples from serum, plasma or other biological fluids. |
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Bovine Immunoglobulin G (IgG) ELISA Kit |
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| DLR-IgG-b-96T | DL Develop | 96T | 708 EUR |
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Description: A competitive inhibition quantitative ELISA assay kit for detection of Bovine Immunoglobulin G (IgG) in samples from serum, plasma or other biological fluids. |
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Canine Immunoglobulin G (IgG) ELISA Kit |
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| DLR-IgG-c-48T | DL Develop | 48T | 529.2 EUR |
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Description: A competitive inhibition quantitative ELISA assay kit for detection of Canine Immunoglobulin G (IgG) in samples from serum, plasma or other biological fluids. |
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Canine Immunoglobulin G (IgG) ELISA Kit |
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| DLR-IgG-c-96T | DL Develop | 96T | 684 EUR |
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Description: A competitive inhibition quantitative ELISA assay kit for detection of Canine Immunoglobulin G (IgG) in samples from serum, plasma or other biological fluids. |
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Equine Immunoglobulin G (IgG) ELISA Kit |
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| DLR-IgG-Eq-48T | DL Develop | 48T | 432 EUR |
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Description: A competitive inhibition quantitative ELISA assay kit for detection of Equine Immunoglobulin G (IgG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Equine Immunoglobulin G (IgG) ELISA Kit |
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| DLR-IgG-Eq-96T | DL Develop | 96T | 548.4 EUR |
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Description: A competitive inhibition quantitative ELISA assay kit for detection of Equine Immunoglobulin G (IgG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Goat Immunoglobulin G (IgG) ELISA Kit |
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| DLR-IgG-g-48T | DL Develop | 48T | 556.8 EUR |
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Description: A competitive inhibition quantitative ELISA assay kit for detection of Goat Immunoglobulin G (IgG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Goat Immunoglobulin G (IgG) ELISA Kit |
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| DLR-IgG-g-96T | DL Develop | 96T | 721.2 EUR |
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Description: A competitive inhibition quantitative ELISA assay kit for detection of Goat Immunoglobulin G (IgG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Human Immunoglobulin G (IgG) ELISA Kit |
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| DLR-IgG-Hu-48T | DL Develop | 48T | 286.8 EUR |
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Description: A competitive inhibition quantitative ELISA assay kit for detection of Human Immunoglobulin G (IgG) in samples from serum, plasma or other biological fluids. |
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Human Immunoglobulin G (IgG) ELISA Kit |
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| DLR-IgG-Hu-96T | DL Develop | 96T | 350.4 EUR |
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Description: A competitive inhibition quantitative ELISA assay kit for detection of Human Immunoglobulin G (IgG) in samples from serum, plasma or other biological fluids. |
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Mouse Immunoglobulin G (IgG) ELISA Kit |
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| DLR-IgG-Mu-48T | DL Develop | 48T | 393.6 EUR |
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Description: A competitive inhibition quantitative ELISA assay kit for detection of Mouse Immunoglobulin G (IgG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Mouse Immunoglobulin G (IgG) ELISA Kit |
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| DLR-IgG-Mu-96T | DL Develop | 96T | 498 EUR |
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Description: A competitive inhibition quantitative ELISA assay kit for detection of Mouse Immunoglobulin G (IgG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Porcine Immunoglobulin G (IgG) ELISA Kit |
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| DLR-IgG-p-48T | DL Develop | 48T | 547.2 EUR |
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Description: A competitive inhibition quantitative ELISA assay kit for detection of Porcine Immunoglobulin G (IgG) in samples from serum, plasma, tissue homogenates or other biological fluids. |
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Porcine Immunoglobulin G (IgG) ELISA Kit |
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| DLR-IgG-p-96T | DL Develop | 96T | 708 EUR |
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Description: A competitive inhibition quantitative ELISA assay kit for detection of Porcine Immunoglobulin G (IgG) in samples from serum, plasma, tissue homogenates or other biological fluids. |
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Rat Immunoglobulin G (IgG) ELISA Kit |
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| DLR-IgG-Ra-48T | DL Develop | 48T | 400.8 EUR |
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Description: A competitive inhibition quantitative ELISA assay kit for detection of Rat Immunoglobulin G (IgG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Rat Immunoglobulin G (IgG) ELISA Kit |
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| DLR-IgG-Ra-96T | DL Develop | 96T | 506.4 EUR |
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Description: A competitive inhibition quantitative ELISA assay kit for detection of Rat Immunoglobulin G (IgG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Rabbit Immunoglobulin G (IgG) ELISA Kit |
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| DLR-IgG-Rb-48T | DL Develop | 48T | 404.4 EUR |
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Description: A competitive inhibition quantitative ELISA assay kit for detection of Rabbit Immunoglobulin G (IgG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Rabbit Immunoglobulin G (IgG) ELISA Kit |
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| DLR-IgG-Rb-96T | DL Develop | 96T | 511.2 EUR |
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Description: A competitive inhibition quantitative ELISA assay kit for detection of Rabbit Immunoglobulin G (IgG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Bovine Immunoglobulin G (IgG) ELISA Kit |
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| RDR-IgG-b-48Tests | Reddot Biotech | 48 Tests | 567.6 EUR |
Bovine Immunoglobulin G (IgG) ELISA Kit |
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| RDR-IgG-b-96Tests | Reddot Biotech | 96 Tests | 784.8 EUR |
Canine Immunoglobulin G (IgG) ELISA Kit |
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| RDR-IgG-c-48Tests | Reddot Biotech | 48 Tests | 546 EUR |
Canine Immunoglobulin G (IgG) ELISA Kit |
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| RDR-IgG-c-96Tests | Reddot Biotech | 96 Tests | 754.8 EUR |
Equine Immunoglobulin G (IgG) ELISA Kit |
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| RDR-IgG-Eq-48Tests | Reddot Biotech | 48 Tests | 429.6 EUR |
Equine Immunoglobulin G (IgG) ELISA Kit |
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| RDR-IgG-Eq-96Tests | Reddot Biotech | 96 Tests | 588 EUR |
Goat Immunoglobulin G (IgG) ELISA Kit |
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| RDR-IgG-g-48Tests | Reddot Biotech | 48 Tests | 578.4 EUR |
Goat Immunoglobulin G (IgG) ELISA Kit |
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| RDR-IgG-g-96Tests | Reddot Biotech | 96 Tests | 800.4 EUR |
Human Immunoglobulin G (IgG) ELISA Kit |
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| RDR-IgG-Hu-48Tests | Reddot Biotech | 48 Tests | 259.2 EUR |
Human Immunoglobulin G (IgG) ELISA Kit |
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| RDR-IgG-Hu-96Tests | Reddot Biotech | 96 Tests | 344.4 EUR |
Mouse Immunoglobulin G (IgG) ELISA Kit |
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| RDR-IgG-Mu-48Tests | Reddot Biotech | 48 Tests | 385.2 EUR |
Mouse Immunoglobulin G (IgG) ELISA Kit |
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| RDR-IgG-Mu-96Tests | Reddot Biotech | 96 Tests | 525.6 EUR |
Porcine Immunoglobulin G (IgG) ELISA Kit |
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| RDR-IgG-p-48Tests | Reddot Biotech | 48 Tests | 567.6 EUR |
Porcine Immunoglobulin G (IgG) ELISA Kit |
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| RDR-IgG-p-96Tests | Reddot Biotech | 96 Tests | 784.8 EUR |
Rat Immunoglobulin G (IgG) ELISA Kit |
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| RDR-IgG-Ra-48Tests | Reddot Biotech | 48 Tests | 393.6 EUR |
Rat Immunoglobulin G (IgG) ELISA Kit |
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| RDR-IgG-Ra-96Tests | Reddot Biotech | 96 Tests | 536.4 EUR |
Rabbit Immunoglobulin G (IgG) ELISA Kit |
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| RDR-IgG-Rb-48Tests | Reddot Biotech | 48 Tests | 397.2 EUR |
Rabbit Immunoglobulin G (IgG) ELISA Kit |
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| RDR-IgG-Rb-96Tests | Reddot Biotech | 96 Tests | 541.2 EUR |
Bovine Immunoglobulin G (IgG) ELISA Kit |
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| RD-IgG-b-48Tests | Reddot Biotech | 48 Tests | 543.6 EUR |
Bovine Immunoglobulin G (IgG) ELISA Kit |
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| RD-IgG-b-96Tests | Reddot Biotech | 96 Tests | 750 EUR |
Porcine Immunoglobulin G (IgG) ELISA Kit |
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| RD-IgG-p-48Tests | Reddot Biotech | 48 Tests | 543.6 EUR |
Porcine Immunoglobulin G (IgG) ELISA Kit |
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| RD-IgG-p-96Tests | Reddot Biotech | 96 Tests | 750 EUR |
Rat Immunoglobulin G (IgG) ELISA Kit |
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| RD-IgG-Ra-48Tests | Reddot Biotech | 48 Tests | 376.8 EUR |
Rat Immunoglobulin G (IgG) ELISA Kit |
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| RD-IgG-Ra-96Tests | Reddot Biotech | 96 Tests | 513.6 EUR |
Western Blotting Mouse IgG DAB Chromogenic Reagent Kit (Yellow) |
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| SA2020 | BosterBio | 1 kit(1200 cm2) | 169.2 EUR |
Western Blotting Goat IgG DAB Chromogenic Reagent Kit (Yellow) |
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| SA2021 | BosterBio | 1 kit(1200 cm2) | 169.2 EUR |
Western Blotting Rabbit IgG DAB Chromogenic Reagent Kit (Yellow) |
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| SA2022 | BosterBio | 1 kit(1200 cm2) | 169.2 EUR |
Western Blotting Rat IgG DAB Chromogenic Reagent Kit (Yellow) |
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| SA2023 | BosterBio | 1 kit(1200 cm2) | 169.2 EUR |
Western Blotting Mouse IgG DAB Chromogenic Reagent Kit (Blue) |
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| SA2024 | BosterBio | 1 kit(1200 cm2) | 169.2 EUR |
Western Blotting Rabbit IgG DAB Chromogenic Reagent Kit (Blue) |
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| SA2025 | BosterBio | 1 kit(1200 cm2) | 169.2 EUR |
Guinea pig Immunoglobulin G (IgG) ELISA Kit |
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| DLR-IgG-Gu-48T | DL Develop | 48T | 378 EUR |
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Description: A competitive inhibition quantitative ELISA assay kit for detection of Guinea pig Immunoglobulin G (IgG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Guinea pig Immunoglobulin G (IgG) ELISA Kit |
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| DLR-IgG-Gu-96T | DL Develop | 96T | 475.2 EUR |
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Description: A competitive inhibition quantitative ELISA assay kit for detection of Guinea pig Immunoglobulin G (IgG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Guinea pig Immunoglobulin G (IgG) ELISA Kit |
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| RDR-IgG-Gu-48Tests | Reddot Biotech | 48 Tests | 366 EUR |
Guinea pig Immunoglobulin G (IgG) ELISA Kit |
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| RDR-IgG-Gu-96Tests | Reddot Biotech | 96 Tests | 498 EUR |
BOP reagent |
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| 5-02141 | CHI Scientific | 25g | Ask for price |
BOP reagent |
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| 5-02142 | CHI Scientific | 100g | Ask for price |
Chymase reagent |
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| 30C-CP1129 | Fitzgerald | 5 units | 2622 EUR |
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Description: Purified native Human Chymase reagent |
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Traut's Reagent |
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| 2330-1000 | Biovision | 418.8 EUR | |
Traut's Reagent |
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| 2330-500 | Biovision | 248.4 EUR | |
MTS Reagent |
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| 2808-1000 | Biovision | 1188 EUR | |
MTS Reagent |
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| 2808-250 | Biovision | 438 EUR | |
MTT Reagent |
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| 2809-1G | Biovision | 216 EUR | |
MTT Reagent |
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| 2809-5G | Biovision | 652.8 EUR | |
BOP reagent |
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| A7015-100000 | ApexBio | 100 g | 240 EUR |
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Description: A peptide coupling reagent. Can be used in the preparation of phenyl esters of amino acids which have been shown to be valuable as blocked derivatives of amino acids in the field of peptide synthesis. |
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BOP reagent |
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| A7015-25000 | ApexBio | 25 g | 135.6 EUR |
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Description: A peptide coupling reagent. Can be used in the preparation of phenyl esters of amino acids which have been shown to be valuable as blocked derivatives of amino acids in the field of peptide synthesis. |
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Bradford reagent |
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| BDE641 | Bio Basic | 100ml | 73.21 EUR |
Bluing Reagent |
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| BRT030 | ScyTek Laboratories | 30 ml | 72 EUR |
Bluing Reagent |
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| BRT125 | ScyTek Laboratories | 125 ml | 75.6 EUR |
Bluing Reagent |
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| BRT3800 | ScyTek Laboratories | 1 Gal. | 220.8 EUR |
Bluing Reagent |
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| BRT500 | ScyTek Laboratories | 500 ml | 91.2 EUR |
Bluing Reagent |
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| BRT999 | ScyTek Laboratories | 1000 ml | 105.6 EUR |
Beaucage reagent |
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| HY-100951 | MedChemExpress | 10mM/1mL | 151.2 EUR |
Phosphate Reagent |
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| 106199 | Scientific Laboratory Supplies | PK100 | 61.29 EUR |
Chromium Reagent |
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| 1206699 | Scientific Laboratory Supplies | EACH | 141.72 EUR |
Overlapping strands of those intermediate merchandise hybridize at this 3′ region in a subsequent PCR and are prolonged to generate the full-length product amplified by flanking primers that may embrace restriction enzyme websites for inserting the product into an expression vector for cloning functions. The extremely environment friendly technology of mutant or chimeric genes by this technique can simply be completed with normal laboratory reagents in roughly 1 week.