Due to sturdy electrical fields at the floor, the absorption and scattering of electromagnetic radiation by noble metallic nanoparticles are strongly enhanced. These distinctive properties present the potential of designing novel optically energetic reagents for simultaneous molecular imaging and photothermal most cancers therapy. It is fascinating to make use of brokers which are energetic in the near-infrared (NIR) region of the radiation spectrum to attenuate the gentle extinction by intrinsic chromophores in native tissue. Gold nanorods with appropriate side ratios (size divided by width) can soak up and scatter strongly in the NIR region (650-900 nm).
In the current work, we offer an in vitro demonstration of gold nanorods as novel distinction brokers for each molecular imaging and photothermal most cancers therapy. Nanorods are synthesized and conjugated to anti-epidermal progress issue receptor (anti-EGFR) monoclonal antibodies and incubated in cell cultures with a nonmalignant epithelial cell line (HaCat) and two malignant oral epithelial cell traces (HOC 313 clone 8 and HSC 3). The anti-EGFR antibody-conjugated nanorods bind particularly to the floor of the malignant-type cells with a a lot increased affinity attributable to the overexpressed EGFR on the cytoplasmic membrane of the malignant cells.
As a results of the strongly scattered crimson gentle from gold nanorods in darkish discipline, noticed using a laboratory microscope, the malignant cells are clearly visualized and recognized from the nonmalignant cells. It is discovered that, after publicity to steady crimson laser at 800 nm, malignant cells require about half the laser power to be photothermally destroyed than the nonmalignant cells. Thus, each environment friendly most cancers cell diagnostics and selective photothermal therapy are realized at the identical time.
Production of monoclonal antibodies to group A erythrocytes, HLA and different human cell floor antigens-new instruments for genetic evaluation.
Antibody-secreting hybrid cells have been derived from a fusion between mouse myeloma cells and spleen cells from a mouse immunized with membrane from human tonsil lymphocyte preparations. Hybrids secreting antibodies to cell floor antigens have been detected by assaying tradition supernatants for antibody binding to human tonsil cells. Six completely different antibodies (referred to as W6/1, /28, /32, /34, /45 and /46 have been analyzed.
These have been both towards antigens of huge tissue distribution (W6/32, /34, and /46) or primarily on erythrocytes (W6/1 and W6/28). One of the anti-erythrocyte antibodies (W6/1) detected a polymorphic antigen, since blood group A1 and A2 erythrocytes have been labeled whereas B and O weren’t.
Antibodies W6/34, /45 and /46 have been all towards antigens which have been mapped to the quick arm of chromosome 11 by segregation evaluation of mouse-human hybrids. Immunoprecipitation research recommend that W6/45 antigen could also be a protein of 16,000 dalton, obvious molecular weight, whereas W6/34 and /46 antigens couldn’t be detected by this method.
Antibody W6/32 is towards a determinant widespread to most, if not all, of the 43,000 dalton molecular weight chains of HLA-A, B and C antigens. This was established by somatic cell genetic strategies and by immunoprecipitation evaluation. Tonsil leucocytes certain 370,000 W6/32 antibody molecules per cell at saturation. The hybrid myelomas W6/32 and W6/34 have been cloned, and each secrete an IgG2 antibody.
W6/32 cells have been grown in mice, and the serum of the tumor-bearing animals contained higher than 10 mg/ml of monoclonal antibody. The experiments established the usefulness of the bybrid myeloma approach in making ready monospecific antibodies towards human cell floor antigens. In explicit, this research highlights the potentialities not solely of acquiring reagents for somatic cell genetics, but additionally of acquiring mouse antibodies detecting human antigenic polymorphisms.
Sulforhodamine B colorimetric assay for cytotoxicity screening.
The sulforhodamine B (SRB) assay is used for cell density dedication, based mostly on the measurement of mobile protein content material. The technique described right here has been optimized for the toxicity screening of compounds to adherent cells in a 96-well format.
After an incubation interval, cell monolayers are mounted with 10% (wt/vol) trichloroacetic acid and stained for 30 min, after which the extra dye is eliminated by washing repeatedly with 1% (vol/vol) acetic acid. The protein-bound dye is dissolved in 10 mM Tris base resolution for OD dedication at 510 nm using a microplate reader.
The outcomes are linear over a 20-fold vary of cell numbers and the sensitivity is corresponding to these of fluorometric strategies. The technique not solely permits a lot of samples to be examined inside just a few days, but additionally requires solely easy gear and cheap reagents. The SRB assay is subsequently an environment friendly and extremely cost-effective technique for screening.
Gene splicing and mutagenesis by PCR-driven overlap extension.
Extension of overlapping gene segments by PCR is an easy, versatile approach for site-directed mutagenesis and gene splicing. Initial PCRs generate overlapping gene segments which are then used as template DNA for an additional PCR to create a full-length product. Internal primers generate overlapping, complementary 3′ ends on the intermediate segments and introduce nucleotide substitutions, insertions or deletions for site-directed mutagenesis, or for gene splicing, encode the nucleotides discovered at the junction of adjoining gene segments.
EP Reagent Methoxyphenylacetic Reagent |
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| 1053601 | Scientific Laboratory Supplies | 100ML | 435.6 EUR |
EP Reagent Sulfomolybdic Reagent R3 |
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| 1086500 | Scientific Laboratory Supplies | 1L | 354 EUR |
EP Reagent Phosphomolybdotungstic Reagent |
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| 1065000 | Scientific Laboratory Supplies | 100ML | 201.6 EUR |
Enhanced Chemiluminescent Reagent Kit (Anti-Rat IgG) |
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| EK1004 | BosterBio | 1 kit (1000 cm2) | 182.4 EUR |
Enhanced Chemiluminescent Reagent Kit (Anti-Goat IgG) |
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| EK1003 | BosterBio | 1 kit (1000 cm2) | 182.4 EUR |
Enhanced Chemiluminescent Reagent Kit (Anti-Mouse IgG) |
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| EK1001 | BosterBio | 1 kit (1000 cm2) | 182.4 EUR |
Enhanced Chemiluminescent Reagent Kit (Anti-Rabbit IgG) |
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| EK1002 | BosterBio | 1 kit (1000 cm2) | 182.4 EUR |
MTS Reagent |
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| 2808-1000 | Biovision | each | 1188 EUR |
MTS Reagent |
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| 2808-250 | Biovision | each | 438 EUR |
MTT Reagent |
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| 2809-1G | Biovision | each | 216 EUR |
MTT Reagent |
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| 2809-5G | Biovision | each | 652.8 EUR |
BOP reagent |
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| 5-02141 | CHI Scientific | 25g | Ask for price |
BOP reagent |
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| 5-02142 | CHI Scientific | 100g | Ask for price |
BOP reagent |
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| A7015-100000 | ApexBio | 100 g | 240 EUR |
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Description: A peptide coupling reagent. Can be used in the preparation of phenyl esters of amino acids which have been shown to be valuable as blocked derivatives of amino acids in the field of peptide synthesis. |
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BOP reagent |
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| A7015-25000 | ApexBio | 25 g | 135.6 EUR |
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Description: A peptide coupling reagent. Can be used in the preparation of phenyl esters of amino acids which have been shown to be valuable as blocked derivatives of amino acids in the field of peptide synthesis. |
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Traut's Reagent |
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| 2330-1000 | Biovision | each | 418.8 EUR |
Traut's Reagent |
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| 2330-500 | Biovision | each | 248.4 EUR |
VeZol Reagent |
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| R411-01 | Vazyme | 100 ml | 76.4 EUR |
VeZol Reagent |
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| R411-02 | Vazyme | 200 ml | 127.6 EUR |
Bluing Reagent |
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| BRT030 | ScyTek Laboratories | 30 ml | 72 EUR |
Bluing Reagent |
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| BRT125 | ScyTek Laboratories | 125 ml | 75.6 EUR |
Bluing Reagent |
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| BRT3800 | ScyTek Laboratories | 1 Gal. | 220.8 EUR |
Bluing Reagent |
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| BRT500 | ScyTek Laboratories | 500 ml | 91.2 EUR |
Bluing Reagent |
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| BRT999 | ScyTek Laboratories | 1000 ml | 105.6 EUR |
Schiff's Reagent |
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| 40120082-1 | Bio-WORLD | 100 mL | 35.14 EUR |
Schiff's Reagent |
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| 40120082-2 | Bio-WORLD | 500 mL | 54.54 EUR |
Chymase reagent |
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| 30C-CP1129 | Fitzgerald | 5 units | 2622 EUR |
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Description: Purified native Human Chymase reagent |
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BURGESS REAGENT |
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| 702010 | Survival Technologies | each | Ask for price |
Nessler Reagent |
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| NESSR | Scientific Laboratory Supplies | 500ML | 182.4 EUR |
FreeZol Reagent |
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| R711-01 | Vazyme | 100 ml | 71.2 EUR |
FreeZol Reagent |
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| R711-02 | Vazyme | 200 ml | 127.2 EUR |
Chromium Reagent |
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| 1206699 | Scientific Laboratory Supplies | EACH | 157.2 EUR |
Bradford reagent |
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| BDE641 | Bio Basic | 100ml | 73.21 EUR |
Beaucage reagent |
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| HY-100951 | MedChemExpress | 10mM/1mL | 151.2 EUR |
Barfoeds Reagent |
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| RRSP6363-D | Atom Scientific | 500ml | 10.8 EUR |
Bradford Reagent |
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| GT1202-1 | Glentham Life Sciences | 1 | 165.7 EUR |
Bradford Reagent |
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| GT1202-500 | Glentham Life Sciences | 500 | 105.5 EUR |
Beaucage reagent |
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| C6991-1000 | ApexBio | 1g | 105 EUR |
Beaucage reagent |
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| C6991-250 | ApexBio | 250mg | 50 EUR |
PI Reagent (50μg/mL) |
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| E-CK-A161-100T | Elabscience Biotech | 100T | 15 EUR |
PI Reagent (50μg/mL) |
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| E-CK-A161-200T | Elabscience Biotech | 200T | 25 EUR |
PI Reagent (50μg/mL) |
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| E-CK-A161-500T | Elabscience Biotech | 500T | 55 EUR |
PI Reagent (50μg/mL) |
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| E-CK-A161-50T | Elabscience Biotech | 50T | 10 EUR |
Cod Reagent 0-15000Ppm |
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| 24159-51 | Scientific Laboratory Supplies | PK25 | 116.4 EUR |
Phosphate Reagent |
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| 106199 | Scientific Laboratory Supplies | PK100 | 69.6 EUR |
Ninhydrin Reagent |
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| MIC6746 | Scientific Laboratory Supplies | EACH | 31.12 EUR |
Western Blotting Rat IgG DAB Chromogenic Reagent Kit (Yellow) |
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| SA2023 | BosterBio | 1 kit(1200 cm2) | 169.2 EUR |
Western Blotting Mouse IgG DAB Chromogenic Reagent Kit (Blue) |
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| SA2024 | BosterBio | 1 kit(1200 cm2) | 169.2 EUR |
COD Reagent (1977 method) |
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| WTR50W | Scientific Laboratory Supplies | 2.5L | 135.6 EUR |
ViralBoost Reagent |
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| VB100 | ALSTEM | 1ml | 176.4 EUR |
Western Blotting Goat IgG DAB Chromogenic Reagent Kit (Yellow) |
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| SA2021 | BosterBio | 1 kit(1200 cm2) | 169.2 EUR |
Western Blotting Rabbit IgG DAB Chromogenic Reagent Kit (Blue) |
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| SA2025 | BosterBio | 1 kit(1200 cm2) | 169.2 EUR |
DAPI Reagent (25μg/mL) |
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| E-CK-A163-100T | Elabscience Biotech | 100T | 15 EUR |
DAPI Reagent (25μg/mL) |
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| E-CK-A163-200T | Elabscience Biotech | 200T | 25 EUR |
DAPI Reagent (25μg/mL) |
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| E-CK-A163-500T | Elabscience Biotech | 500T | 55 EUR |
DAPI Reagent (25μg/mL) |
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| E-CK-A163-50T | Elabscience Biotech | 50T | 10 EUR |
Western Blotting Mouse IgG DAB Chromogenic Reagent Kit (Yellow) |
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| SA2020 | BosterBio | 1 kit(1200 cm2) | 169.2 EUR |
ECL Start Reagent 2000cm2 |
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| RPN3243 | Scientific Laboratory Supplies | EACH | 138 EUR |
ECL Start Reagent 4000cm2 |
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| RPN3244 | Scientific Laboratory Supplies | EACH | 222 EUR |
Western Blotting Rabbit IgG DAB Chromogenic Reagent Kit (Yellow) |
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| SA2022 | BosterBio | 1 kit(1200 cm2) | 169.2 EUR |
BCA Reagent, 16ML |
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| C144-16ML | Arbor Assays | 16ML | 132 EUR |
TDA Reagent - 10mL |
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| MIC6732 | Scientific Laboratory Supplies | EACH | 26.33 EUR |
PYR Reagent - 10mL |
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| MIC6744 | Scientific Laboratory Supplies | EACH | 31.12 EUR |
Thioacetamide Reagent |
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| THIOR01 | Scientific Laboratory Supplies | 100ML | 99.6 EUR |
Girard's reagent T |
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| 20-abx184099 | Abbexa |
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Silica 3 Reagent |
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| 27169 | Scientific Laboratory Supplies | 100ML | 99.6 EUR |
BSA (Reagent Grade) |
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| 30-AB79 | Fitzgerald | 1 kg | 1862.4 EUR |
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Description: Reagent Grade Bovine Serum Albumin (99% pure) |
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BSA (Reagent Grade) |
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| 30-AB81 | Fitzgerald | 200 grams | 571.2 EUR |
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Description: Reagent Grade Sulphydryl Blocked BSA (99% pure) |
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Tri-RNA Reagent |
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| FATRR-001 | Favorgen | 100ml | 283.2 EUR |
Tri-RNA Reagent |
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| FATRR-002 | Favorgen | 50ml | 211.2 EUR |
Tri-RNA Reagent |
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| FATRR-003 | Favorgen | 450ml | 774 EUR |
Schiff''s reagent |
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| GT3929-1 | Glentham Life Sciences | 1 | 113.1 EUR |
Schiff''s reagent |
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| GT3929-250 | Glentham Life Sciences | 250 | 37.7 EUR |
Griess Reagent R2 |
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| 40700088-1 | Bio-WORLD | 8 mL | 26 EUR |
Griess Reagent R2 |
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| 40700088-2 | Bio-WORLD | 50 mL | 97.49 EUR |
Griess Reagent R1 |
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| 40720061-1 | Bio-WORLD | 8 mL | 26 EUR |
Griess Reagent R1 |
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| 40720061-2 | Bio-WORLD | 50 mL | 97.49 EUR |
Tri-RNA Reagent |
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| FATRR_001 | Favorgen | 1 kit | 122.5 EUR |
Tri-RNA Reagent |
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| FATRR_002 | Favorgen | 1 kit | 59.5 EUR |
7-AAD Reagent (100μg/mL) |
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| E-CK-A162-100T | Elabscience Biotech | 100T | 30 EUR |
7-AAD Reagent (100μg/mL) |
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| E-CK-A162-200T | Elabscience Biotech | 200T | 50 EUR |
7-AAD Reagent (100μg/mL) |
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| E-CK-A162-500T | Elabscience Biotech | 500T | 110 EUR |
7-AAD Reagent (100μg/mL) |
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| E-CK-A162-50T | Elabscience Biotech | 50T | 20 EUR |
ECL Start Reagent 200cm2 |
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| RPN3245 | Scientific Laboratory Supplies | EACH | 26.4 EUR |
EP Reagent Ferroin |
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| 1038100 | Scientific Laboratory Supplies | 100ML | 96 EUR |
Griess Reagent Kit |
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| 30100 | Biotium | 1KIT | 178.8 EUR |
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Description: Minimum order quantity: 1 unit of 1KIT |
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Biotin reagent (HRP) |
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| 65C-CE0202 | Fitzgerald | 5 mg | 292.8 EUR |
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Description: HRP conjugated biotin labelling reagent |
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Alcohol, Reagent (70%) |
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| EAS500 | ScyTek Laboratories | 500 ml | 94.8 EUR |
Alcohol, Reagent (70%) |
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| EAS999 | ScyTek Laboratories | 1000 ml | 121.2 EUR |
SulfaVer 4 Reagent |
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| 2106769 | Scientific Laboratory Supplies | PK100 | 78 EUR |
Citric Acid Reagent |
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| 2254232 | Scientific Laboratory Supplies | EACH | 52.8 EUR |
DTT (Cleland's reagent) |
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| DB0058 | Bio Basic | 5g | 101.76 EUR |
DTNB (Ellman's Reagent) |
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| DB0113 | Bio Basic | 5g | 117.42 EUR |
n-Heptane Reagent |
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| HC5400 | Bio Basic | 1L | 94.8 EUR |
Bottle Reagent 500ml Pyrex |
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| B54564 | Scientific Laboratory Supplies | PK10 | 124.8 EUR |
TMB Stop Reagent 450 nm |
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| 21530071-1 | Bio-WORLD | 100 mL | 20.55 EUR |
TMB Stop Reagent 450 nm |
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| 21530071-2 | Bio-WORLD | 500 mL | 43.56 EUR |
TMB Stop Reagent 450 nm |
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| 21530071-3 | Bio-WORLD | 1 L | 63.61 EUR |
Bradford Dye Reagent |
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| 0209R | AthenaES | 100 ml | 157.2 EUR |
DeStreak reagent 1mL |
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| 17600318 | Scientific Laboratory Supplies | EACH | 82.8 EUR |
FcR blocking Reagent |
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| 20-abx290024 | Abbexa |
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Detection Reagent A |
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| abx296004-120ul | Abbexa | 120 ul | 385.2 EUR |
Olsens Mixed Reagent |
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| OLSMR01 | Scientific Laboratory Supplies | 1L | 168 EUR |
Traut's Reagent Buffer |
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| 22050003-1 | Bio-WORLD | 500 mg | 122.03 EUR |
Traut's Reagent Buffer |
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| 22050003-2 | Bio-WORLD | 1 g | 162.17 EUR |
Scott's Bluing Reagent |
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| 24605-1 | Polysciences Europe GmbH | 1gal | 125 EUR |
Scott's Bluing Reagent |
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| 24605-1000 | Polysciences Europe GmbH | 1000ml | Ask for price |
EZ Cap? Reagent AG |
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| B8176-.01 | ApexBio | 10 µl (100 mM) | 369.6 EUR |
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Description: EZ Cap Reagent AG is a co-transcriptional capping reagent for in vitro transcription of 5? capped mRNA which would result in a Cap 1 structure. Cap AG has shown to provide up to 98% capping efficiency in literatures.EZ Cap AG requires an AG initiator. |
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EZ Cap? Reagent AG |
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| B8176-.05 | ApexBio | 50 µl (100 mM) | 1488 EUR |
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Description: EZ Cap Reagent AG is a co-transcriptional capping reagent for in vitro transcription of 5? capped mRNA which would result in a Cap 1 structure. Cap AG has shown to provide up to 98% capping efficiency in literatures.EZ Cap AG requires an AG initiator. |
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EZ Cap? Reagent AG |
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| B8176-.1 | ApexBio | 100 µl (100 mM) | 2656.8 EUR |
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Description: EZ Cap Reagent AG is a co-transcriptional capping reagent for in vitro transcription of 5? capped mRNA which would result in a Cap 1 structure. Cap AG has shown to provide up to 98% capping efficiency in literatures.EZ Cap AG requires an AG initiator. |
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EZ Cap? Reagent GG |
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| B8177-.01 | ApexBio | 10 µl (100 mM) | 369.6 EUR |
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Description: EZ Cap Reagent GG is a co-transcriptional capping reagent for in vitro transcription of 5? capped mRNA resulting in a Cap 1 structure. EZ Cap GG requires a GG initiator. EZ Cap GG typically provides >90% capped material generating a natural Cap 1 structure. |
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Overlapping strands of those intermediate merchandise hybridize at this 3′ region in a subsequent PCR and are prolonged to generate the full-length product amplified by flanking primers that may embrace restriction enzyme websites for inserting the product into an expression vector for cloning functions. The extremely environment friendly technology of mutant or chimeric genes by this technique can simply be completed with normal laboratory reagents in roughly 1 week.