Emerging strategies for the correct quantification of gene expression in particular person cells maintain promise for revealing the extent, operate and origins of cell-to-cell variability. Different high-throughput strategies for single-cell RNA-seq have been launched that fluctuate in protection, sensitivity and multiplexing capability. We lately launched Smart-seq for transcriptome evaluation from single cells, and we subsequently optimized the tactic for improved sensitivity, accuracy and full-length protection throughout transcripts.
Here we current an in depth protocol for Smart-seq2 that enables the era of full-length cDNA and sequencing libraries by using commonplace reagents. The whole protocol takes ∼2 d from cell selecting to having a remaining library prepared for sequencing; sequencing would require an extra 1-Three d relying on the technique and sequencer.
The present limitations are the dearth of strand specificity and the lack to detect nonpolyadenylated (polyA(-)) RNA. Genome modifying pushed by zinc-finger nucleases (ZFNs) yields excessive gene-modification efficiencies (>10%) by introducing a recombinogenic double-strand break into the focused gene. The cleavage occasion is induced using two custom-designed ZFNs that heterodimerize upon binding DNA to type a catalytically lively nuclease advanced. Using the present ZFN structure, nonetheless, cleavage-competent homodimers can also type that may restrict security or efficacy through off-target cleavage.
Here we develop an improved ZFN structure that eliminates this downside. Using structure-based design, we engineer two variant ZFNs that effectively cleave DNA solely when paired as a heterodimer. These ZFNs modify a local endogenous locus as effectively because the parental structure, however with a >40-fold discount in homodimer operate and far decrease ranges of genome-wide cleavage. This structure offers a common means for bettering the specificity of ZFNs as gene modification reagents.
Hyperthermophilic enzymes: sources, makes use of, and molecular mechanisms for thermostability.
Enzymes synthesized by hyperthermophiles (micro organism and archaea with optimum progress temperatures of>> 80 levels C), additionally referred to as hyperthermophilic enzymes, are sometimes thermostable (i.e., immune to irreversible inactivation at excessive temperatures) and are optimally lively at excessive temperatures.
These enzymes share the identical catalytic mechanisms with their mesophilic counterparts. When cloned and expressed in mesophilic hosts, hyperthermophilic enzymes often retain their thermal properties, indicating that these properties are genetically encoded. Sequence alignments, amino acid content material comparisons, crystal construction comparisons, and mutagenesis experiments point out that hyperthermophilic enzymes are, certainly, similar to their mesophilic homologues.
No single mechanism is liable for the exceptional stability of hyperthermophilic enzymes. Increased thermostability should be discovered, as an alternative, in a small variety of extremely particular alterations that usually don’t obey any apparent visitors guidelines. After briefly discussing the range of hyperthermophilic organisms, this evaluate concentrates on the exceptional thermostability of their enzymes.
The biochemical and molecular properties of hyperthermophilic enzymes are described. Mechanisms liable for protein inactivation are reviewed. The molecular mechanisms concerned in protein thermostabilization are mentioned, together with ion pairs, hydrogen bonds, hydrophobic interactions, disulfide bridges, packing, lower of the entropy of unfolding, and intersubunit interactions. Finally, present makes use of and potential functions of thermophilic and hyperthermophilic enzymes as analysis reagents and as catalysts for industrial processes are described.
Nuclear protein import in permeabilized mammalian cells requires soluble cytoplasmic components.
We have developed an in vitro system involving digitonin-permeabilized vertebrate cells to review biochemical occasions within the transport of macromolecules throughout the nuclear envelope. While therapy of cultured cells with digitonin permeabilizes the plasma membranes to macromolecules, the nuclear envelopes stay structurally intact and nuclei retain the power to move and accumulate proteins containing the SV40 giant T antigen nuclear location sequence.
Transport requires addition of exogenous cytosol to permeabilized cells, indicating the soluble cytoplasmic issue(s) required for nuclear import are launched throughout digitonin therapy. In this reconstituted import system, a protein containing a nuclear location sign is quickly amassed in nuclei, the place it reaches a 30-fold focus in comparison with the encircling medium inside 30 min. Nuclear import is particular for a purposeful nuclear location sequence, requires ATP and cytosol, and is temperature dependent.
Furthermore, accumulation of the transport substrate inside nuclei is totally inhibited by wheat germ agglutinin, which binds to nuclear pore complexes and inhibits transport in vivo. Together, these outcomes point out that the permeabilized cell system reproduces genuine nuclear protein import. In a preliminary biochemical dissection of the system, we observe that the sulfhydryl alkylating reagent N-ethylmaleimide inactivates each cytosolic issue(s) and in addition part(s) within the insoluble permeabilized cell fraction required for nuclear protein import.
Because this permeabilized cell mannequin is straightforward, environment friendly, and works successfully with cells and cytosol fractions ready from a wide range of completely different vertebrate sources, it can show highly effective for investigating the biochemical pathway of nuclear transport.
Accurate multiplex polony sequencing of an developed bacterial genome.
We describe a DNA sequencing expertise wherein a generally out there, cheap epifluorescence microscope is transformed to speedy nonelectrophoretic DNA sequencing automation. We apply this expertise to resequence an developed pressure of Escherichia coli at lower than one error per million consensus bases.
SYBR Green qPCR Master Mix (No ROX) |
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| C0006-1mg | TargetMol Chemicals | 1mg | Ask for price |
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Description: SYBR Green qPCR Master Mix (No ROX) |
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SYBR Green qPCR Master Mix (No ROX) |
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| C0006-50mg | TargetMol Chemicals | 50mg | Ask for price |
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Description: SYBR Green qPCR Master Mix (No ROX) |
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SYBR Green qPCR Master Mix (No ROX) |
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| C0006-5mg | TargetMol Chemicals | 5mg | Ask for price |
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Description: SYBR Green qPCR Master Mix (No ROX) |
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SYBR Green qPCR Master Mix (No ROX) |
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| HY-K0523 | MedChemExpress | 5 mL (500 rxns ) | 308.4 EUR |
SYBR Green qPCR Master Mix (Low ROX) |
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| C0008-10mg | TargetMol Chemicals | 10mg | Ask for price |
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Description: SYBR Green qPCR Master Mix (Low ROX) |
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SYBR Green qPCR Master Mix (Low ROX) |
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| C0008-1g | TargetMol Chemicals | 1g | Ask for price |
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Description: SYBR Green qPCR Master Mix (Low ROX) |
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SYBR Green qPCR Master Mix (Low ROX) |
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| C0008-1mg | TargetMol Chemicals | 1mg | Ask for price |
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Description: SYBR Green qPCR Master Mix (Low ROX) |
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SYBR Green qPCR Master Mix (Low ROX) |
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| C0008-50mg | TargetMol Chemicals | 50mg | Ask for price |
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Description: SYBR Green qPCR Master Mix (Low ROX) |
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SYBR Green qPCR Master Mix (Low ROX) |
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| C0008-5mg | TargetMol Chemicals | 5mg | Ask for price |
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Description: SYBR Green qPCR Master Mix (Low ROX) |
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SYBR Green qPCR Master Mix (Low ROX) |
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| HY-K0522 | MedChemExpress | 5 mL (500 rxns ) | 308.4 EUR |
SYBR Green qPCR Master Mix (High ROX) |
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| C0007-10mg | TargetMol Chemicals | 10mg | Ask for price |
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Description: SYBR Green qPCR Master Mix (High ROX) |
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SYBR Green qPCR Master Mix (High ROX) |
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| C0007-1g | TargetMol Chemicals | 1g | Ask for price |
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Description: SYBR Green qPCR Master Mix (High ROX) |
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SYBR Green qPCR Master Mix (High ROX) |
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| C0007-1mg | TargetMol Chemicals | 1mg | Ask for price |
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Description: SYBR Green qPCR Master Mix (High ROX) |
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SYBR Green qPCR Master Mix (High ROX) |
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| C0007-50mg | TargetMol Chemicals | 50mg | Ask for price |
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Description: SYBR Green qPCR Master Mix (High ROX) |
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SYBR Green qPCR Master Mix (High ROX) |
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| C0007-5mg | TargetMol Chemicals | 5mg | Ask for price |
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Description: SYBR Green qPCR Master Mix (High ROX) |
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SYBR Green qPCR Master Mix (High ROX) |
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| HY-K0521 | MedChemExpress | 1 mL (100 rxns) | 135.6 EUR |
2x SYBR Green qPCR Master Mix (Low ROX) |
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| B21702 | Bimake | 5 ml | 268.8 EUR |
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Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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2x SYBR Green qPCR Master Mix (Low ROX) |
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| B21703 | Bimake | 25 ml | 1027.2 EUR |
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Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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2x SYBR Green qPCR Master Mix (High ROX) |
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| B21402 | Bimake | 5 ml | 268.8 EUR |
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Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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2x SYBR Green qPCR Master Mix (High ROX) |
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| B21403 | Bimake | 25 ml | 1027.2 EUR |
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Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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AceQ qPCR SYBR® Green Master Mix |
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| Q111-02 | Vazyme | 500 rxn (20 μl/rxn) | 265.2 EUR |
AceQ qPCR SYBR® Green Master Mix |
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| Q111-03 | Vazyme | 2500 rxn (20 μl/rxn) | 775.2 EUR |
Maxima SYBR Green/ROX qPCR Master Mix - 200 x 25 µL rxns |
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| THK0221 | Westburg | each | Ask for price |
Maxima SYBR Green/ROX qPCR Master Mix - 1,000 x 25 µL rxns |
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| THK0222 | Westburg | each | Ask for price |
Maxima SYBR Green/ROX qPCR Master Mix - 4,000 x 25 µL rxns |
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| THK0223 | Westburg | each | 3324.5 EUR |
Maxima SYBR Green/Fluorescein qPCR Master Mix - 200 x 25 µL rxns |
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| THK0241 | Westburg | each | Ask for price |
Maxima SYBR Green/Fluorescein qPCR Master Mix - 1,000 x 25 µL rxns |
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| THK0242 | Westburg | each | Ask for price |
Maxima SYBR Green/Fluorescein qPCR Master Mix - 4,000 x 25 µL rxns |
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| THK0243 | Westburg | each | Ask for price |
Maxima SYBR Green qPCR Master Mix, ROX Solution provided - 200 x 25 µL rxns |
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| THK0251 | Westburg | each | 232.17 EUR |
Maxima SYBR Green qPCR Master Mix, ROX Solution provided - 1,000 x 25 µL rxns |
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| THK0252 | Westburg | each | 1046.4 EUR |
Maxima SYBR Green qPCR Master Mix, ROX Solution provided - 4,000 x 25 µL rxns |
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| THK0253 | Westburg | each | Ask for price |
BioEasy Master Mix (SYBR Green) |
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| TRI-B25L1 | TRI Biotech | 200T | 403.2 EUR |
SYBR Green cRNA PCR Master Mix |
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| SGM002 | Signosis | 2 ml | 174 EUR |
EnoGene Green qPCR Master Mix |
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| E31M001 | EnoGene | 500 reaction | 75 EUR |
ChamQ SYBR qPCR Master Mix |
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| Q311-03-2500rxns | Vazyme | 2,500 rxns | 562.79 EUR |
ChamQ SYBR qPCR Master Mix |
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| Q311-00-100rxns | Vazyme | 100 rxns | 1.09 EUR |
ChamQ SYBR qPCR Master Mix |
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| Q311-02 | Vazyme | 500 rxns (20 μl/rxn) | 120.55 EUR |
ChamQ SYBR qPCR Master Mix |
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| Q311-02-500rxns | Vazyme | 500 rxns | 124.83 EUR |
ChamQ SYBR qPCR Master Mix |
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| Q311-03 | Vazyme | 2,500 rxns (20 μl/rxn) | 543.5 EUR |
Apex qPCR GREEN Master Mix |
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| 42-116PG | Genesee Scientific | 400 x 25µl Reactions/Unit | 341.44 EUR |
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Description: 2X, Without ROX, 5ml |
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Apex qPCR GREEN Master Mix |
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| 42-117PG | Genesee Scientific | 4000 x 25µl Reactions/Unit | 2926.74 EUR |
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Description: 2X, Without ROX, 50ml |
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Apex qPCR GREEN Master Mix |
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| 42-118PG | Genesee Scientific | 400 x 25µl Reactions/Unit | 338.88 EUR |
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Description: 2X, Low ROX, 5ml |
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Apex qPCR GREEN Master Mix |
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| 42-119PG | Genesee Scientific | 4000 x 25µl Reactions/Unit | 2926.74 EUR |
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Description: 2X, Low ROX, 50ml |
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Apex qPCR GREEN Master Mix |
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| 42-120PG | Genesee Scientific | 400 x 25µl Reactions/Unit | 341.44 EUR |
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Description: 2X, High ROX, 5ml |
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Apex qPCR GREEN Master Mix |
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| 42-121PG | Genesee Scientific | 4000 x 25µl Reactions/Unit | 3095.57 EUR |
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Description: 2X, High ROX, 50ml |
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BioEasy Master Mix (SYBR Green, No ROX) |
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| TRI-B25L1D | TRI Biotech | 200T | 403.2 EUR |
BioEasy Master Mix (SYBR Green, low ROX) |
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| TRI-B25L1C | TRI Biotech | 200T | 403.2 EUR |
BioEasy Master Mix (SYBR Green, high ROX) |
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| TRI-B25L1B | TRI Biotech | 200T | 403.2 EUR |
ChamQ SYBR Color qPCR Master Mix |
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| Q411-00-100rxns | Vazyme | 100 rxns | 1.09 EUR |
ChamQ SYBR Color qPCR Master Mix |
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| Q411-02 | Vazyme | 500 rxns (20 μl/rxn) | 140.63 EUR |
ChamQ SYBR Color qPCR Master Mix |
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| Q411-02-500rxns | Vazyme | 500 rxns | 145.62 EUR |
ChamQ SYBR Color qPCR Master Mix |
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| Q411-03 | Vazyme | 2,500 rxns (20 μl/rxn) | 634 EUR |
ChamQ SYBR Color qPCR Master Mix |
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| Q411-03-2500rxns | Vazyme | 2,500 rxns | 656.51 EUR |
AceQ Universal SYBR qPCR Master Mix |
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| Q511-00-100rxns | Vazyme | 100 rxns | 1.09 EUR |
AceQ Universal SYBR qPCR Master Mix |
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| Q511-02 | Vazyme | 500 rxn (20 μl/rxn) | 152.72 EUR |
AceQ Universal SYBR qPCR Master Mix |
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| Q511-02-500rxns | Vazyme | 500 rxns | 158.14 EUR |
AceQ Universal SYBR qPCR Master Mix |
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| Q511-03 | Vazyme | 2500 rxn (20 μl/rxn) | 688.5 EUR |
AceQ Universal SYBR qPCR Master Mix |
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| Q511-03-2500rxns | Vazyme | 2,500 rxns | 712.94 EUR |
ChamQ Universal SYBR qPCR Master Mix |
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| Q711-00-100rxns | Vazyme | 100 rxns | 1.09 EUR |
ChamQ Universal SYBR qPCR Master Mix |
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| Q711-02 | Vazyme | 500 rxn (20 μl/rxn) | 152.72 EUR |
ChamQ Universal SYBR qPCR Master Mix |
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| Q711-02-500rxns | Vazyme | 500 rxns | 158.14 EUR |
ChamQ Universal SYBR qPCR Master Mix |
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| Q711-03 | Vazyme | 2500 rxn (20 μl/rxn) | 688.5 EUR |
ChamQ Universal SYBR qPCR Master Mix |
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| Q711-03-2500rxns | Vazyme | 2,500 rxns | 712.94 EUR |
miRNA Universal SYBR qPCR Master Mix |
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| MQ101-00-100rxns | Vazyme | 100 rxns | 1.09 EUR |
miRNA Universal SYBR qPCR Master Mix |
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| MQ101-01 | Vazyme | 125 rxn(20 μl/rxn) | 38.9 EUR |
miRNA Universal SYBR qPCR Master Mix |
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| MQ101-01-125rxns | Vazyme | 125 rxns | 40.28 EUR |
miRNA Universal SYBR qPCR Master Mix |
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| MQ101-02 | Vazyme | 500 rxn(20 μl/rxn) | 145 EUR |
miRNA Universal SYBR qPCR Master Mix |
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| MQ101-02-500rxns | Vazyme | 500 rxns | 150.15 EUR |
HotStart™ 2X Green qPCR Master Mix |
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| K1070-25 | ApexBio | 25ml | 392 EUR |
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Description: Reverse Transcription & PCR|qPCR |
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HotStart™ 2X Green qPCR Master Mix |
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| K1070-5 | ApexBio | 5ml | 124 EUR |
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Description: Reverse Transcription & PCR|qPCR |
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HotStart™ 2X Green qPCR Master Mix |
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| K1070-50 | ApexBio | 50ml | 768 EUR |
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Description: Reverse Transcription & PCR|qPCR |
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2×SYBR Green qPCR Mix |
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| E37F021-10x5mL | EnoGene | 10×5mL | 1360 EUR |
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Description: DsDNA, ssDNA, RNA quantitative detection kit |
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2×SYBR Green qPCR Mix |
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| E37F021-5mL | EnoGene | 5mL | 227 EUR |
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Description: Fluorescent quantitative PCR kits |
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2×SYBR Green qPCR Mix |
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| MBS8579840-200Tests | MyBiosource | 200Tests | 285 EUR |
2×SYBR Green qPCR Mix |
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| MBS8579840-50Tests | MyBiosource | 50Tests | 180 EUR |
2×SYBR Green qPCR Mix |
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| MBS8579840-5x200Tests | MyBiosource | 5x200Tests | 1230 EUR |
2×SYBR Green qPCR Mix |
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| MBS8579840-5x50Tests | MyBiosource | 5x50Tests | 770 EUR |
ChamQ SYBR qPCR Master Mix (Without ROX) |
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| Q321-02 | Vazyme | 500 rxns (20 μl/rxn) | 120.55 EUR |
ChamQ SYBR qPCR Master Mix (Without ROX) |
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| Q321-02-500rxns | Vazyme | 500 rxns | 124.83 EUR |
ChamQ SYBR qPCR Master Mix (Without ROX) |
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| Q321-03 | Vazyme | 2,500 rxns (20 μl/rxn) | 543.5 EUR |
ChamQ SYBR qPCR Master Mix (Without ROX) |
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| Q321-03-2500rxns | Vazyme | 2,500 rxns | 562.79 EUR |
Taq Pro Universal SYBR qPCR Master Mix |
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| Q712-00-100rxns | Vazyme | 100 rxns | 1.09 EUR |
Taq Pro Universal SYBR qPCR Master Mix |
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| Q712-02 | Vazyme | 500 rxns (20 μl/rxn) | 161 EUR |
Taq Pro Universal SYBR qPCR Master Mix |
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| Q712-02-500rxns | Vazyme | 500 rxns | 166.72 EUR |
Taq Pro Universal SYBR qPCR Master Mix |
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| Q712-03 | Vazyme | 2,500 rxns (20 μl/rxn) | 725 EUR |
Taq Pro Universal SYBR qPCR Master Mix |
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| Q712-03-2500rxns | Vazyme | 2,500 rxns | 750.74 EUR |
miRNA SYBR Green qPCR Mix |
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| 4308A | Unibiotest | 100 Tests*20μL | 80 EUR |
miRNA SYBR Green qPCR Mix |
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| 4308B | Unibiotest | 200 Tests*20μL | 150 EUR |
miRNA SYBR Green qPCR Mix |
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| 4308C | Unibiotest | 500 Tests*20μL | 300 EUR |
ViPrimePLUSHigh 5X One Step RT-qPCR Green Master Mix I (SYBR® Green Dye), 100rxns |
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| QLMM10 | Vivantis | each | Ask for price |
HotStart™ 2X FAST Green qPCR Master Mix |
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| K1171-25 | ApexBio | 25ml | 424 EUR |
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Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
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HotStart™ 2X FAST Green qPCR Master Mix |
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| K1171-5 | ApexBio | 5ml | 132 EUR |
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Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
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HotStart™ 2X FAST Green qPCR Master Mix |
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| K1171-50 | ApexBio | 50ml | 826.4 EUR |
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Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
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HotStart™ Universal 2X Green qPCR Master Mix |
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| K1170-25 | ApexBio | 25ml | 392 EUR |
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Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
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HotStart™ Universal 2X Green qPCR Master Mix |
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| K1170-5 | ApexBio | 5ml | 124 EUR |
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Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
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HotStart™ Universal 2X Green qPCR Master Mix |
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| K1170-50 | ApexBio | 50ml | 752 EUR |
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Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
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ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
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| Q331-02 | Vazyme | 500 rxns (20 μl/rxn) | 120.55 EUR |
ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
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| Q331-02-500rxns | Vazyme | 500 rxns | 124.83 EUR |
ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
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| Q331-03 | Vazyme | 2,500 rxns (20 μl/rxn) | 543.5 EUR |
ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
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| Q331-03-2500rxns | Vazyme | 2,500 rxns | 562.79 EUR |
ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
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| Q341-02 | Vazyme | 500 rxns (20 μl/rxn) | 120.55 EUR |
ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
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| Q341-02-500rxns | Vazyme | 500 rxns | 124.83 EUR |
ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
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| Q341-03 | Vazyme | 2,500 rxns (20 μl/rxn) | 543.5 EUR |
ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
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| Q341-03-2500rxns | Vazyme | 2,500 rxns | 562.79 EUR |
ChamQ SYBR Color qPCR Master Mix (Without ROX) |
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| Q421-02 | Vazyme | 500 rxns (20 μl/rxn) | 140.63 EUR |
ChamQ SYBR Color qPCR Master Mix (Without ROX) |
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| Q421-02-500rxns | Vazyme | 500 rxns | 145.62 EUR |
ChamQ SYBR Color qPCR Master Mix (Without ROX) |
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| Q421-03 | Vazyme | 2,500 rxns (20 μl/rxn) | 634 EUR |
ChamQ SYBR Color qPCR Master Mix (Without ROX) |
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| Q421-03-2500rxns | Vazyme | 2,500 rxns | 656.51 EUR |
HotStart™ Universal 2X FAST Green qPCR Master Mix |
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| K1172-25 | ApexBio | 25ml | 424 EUR |
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Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
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HotStart™ Universal 2X FAST Green qPCR Master Mix |
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| K1172-5 | ApexBio | 5ml | 132 EUR |
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Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
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HotStart™ Universal 2X FAST Green qPCR Master Mix |
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| K1172-50 | ApexBio | 50ml | 826.4 EUR |
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Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
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ViPrimePLUS Taq qPCR Green Master Mix I, 150rxns |
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| QLMM12 | Vivantis | each | Ask for price |
ViPrimePLUS Taq qPCR Green Master Mix II, 150rxns |
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| QLMM17 | Vivantis | each | Ask for price |
ViPrimePLUS AtTaq qPCR Green Master Mix I, 150rxns |
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| QLMM02 | Vivantis | each | Ask for price |
ViPrimePLUS AtTaq qPCR Green Master Mix II, 150rxns |
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| QLMM07 | Vivantis | each | Ask for price |
Hieff® miRNA Universal qPCR SYBR Master Mix |
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| 11171ES03 | Yeasen Biotechnology | 1 mL | 35 EUR |
Hieff® miRNA Universal qPCR SYBR Master Mix |
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| 11171ES08 | Yeasen Biotechnology | 5 mL | 150 EUR |
ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed) |
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| Q431-02 | Vazyme | 500 rxns (20 μl/rxn) | 140.63 EUR |
ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed) |
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| Q431-02-500rxns | Vazyme | 500 rxns | 145.62 EUR |
ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed) |
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| Q431-03 | Vazyme | 2,500 rxns (20 μl/rxn) | 634 EUR |
ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed) |
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| Q431-03-2500rxns | Vazyme | 2,500 rxns | 656.51 EUR |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
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| 17270-1mL | AAT Bioquest | 1 mL | 99 EUR |
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Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
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| 17271 | AAT Bioquest | 5 mL | 508 EUR |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
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| 17271-5mL | AAT Bioquest | 5 mL | 308 EUR |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) |
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| Q441-02 | Vazyme | 500 rxns (20 μl/rxn) | 140.63 EUR |
ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) |
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| Q441-02-500rxns | Vazyme | 500 rxns | 145.62 EUR |
ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) |
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| Q441-03 | Vazyme | 2,500 rxns (20 μl/rxn) | 634 EUR |
ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) |
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| Q441-03-2500rxns | Vazyme | 2,500 rxns | 656.51 EUR |
ABsolute SYBR Green QPCR Mix 800 rxns |
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| AB-1158/B | Westburg | each | Ask for price |
ABsolute SYBR Green QPCR Mix 200 rxns |
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| AB-1159 | Westburg | each | 529.74 EUR |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
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| 17272 | AAT Bioquest | 1 mL | 203 EUR |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
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| 17272-1mL | AAT Bioquest | 1 mL | 99 EUR |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
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| 17273 | AAT Bioquest | 5 mL | 508 EUR |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
|||
| 17273-5mL | AAT Bioquest | 5 mL | 308 EUR |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
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| 17274 | AAT Bioquest | 1 mL | 203 EUR |
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Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
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| 17274-1mL | AAT Bioquest | 1 mL | 99 EUR |
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Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
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| 17275 | AAT Bioquest | 5 mL | 508 EUR |
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Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
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| 17275-5mL | AAT Bioquest | 5 mL | 308 EUR |
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Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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2×SYBR Green qPCR Mix(including ROX) |
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| E37F022-10x5mL | EnoGene | 10×5mL | 1360 EUR |
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Description: Fluorescent quantitative PCR kits |
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2×SYBR Green qPCR Mix(including ROX) |
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| E37F022-5mL | EnoGene | 5mL | 227 EUR |
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Description: Fluorescent quantitative PCR kits |
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2×SYBR Green qPCR Mix (including ROX) |
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| MBS8579841-200Tests | MyBiosource | 200Tests | 285 EUR |
2×SYBR Green qPCR Mix (including ROX) |
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| MBS8579841-50Tests | MyBiosource | 50Tests | 180 EUR |
2×SYBR Green qPCR Mix (including ROX) |
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| MBS8579841-5x200Tests | MyBiosource | 5x200Tests | 1230 EUR |
2×SYBR Green qPCR Mix (including ROX) |
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| MBS8579841-5x50Tests | MyBiosource | 5x50Tests | 770 EUR |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
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| 17276 | AAT Bioquest | 1 mL | 203 EUR |
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Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
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| 17276-1mL | AAT Bioquest | 1 mL | 99 EUR |
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Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
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| 17277 | AAT Bioquest | 5 mL | 508 EUR |
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Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
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| 17277-5mL | AAT Bioquest | 5 mL | 308 EUR |
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Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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Hieff UNICON® Universal Blue qPCR SYBR Master Mix |
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| 11184ES03 | Yeasen Biotechnology | 1 mL | 55 EUR |
Hieff UNICON® Universal Blue qPCR SYBR Master Mix |
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| 11184ES08 | Yeasen Biotechnology | 5×1mL | 245 EUR |
Hieff UNICON® Universal Blue qPCR SYBR Master Mix |
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| 11184ES25 | Yeasen Biotechnology | 5×5mL | 1040 EUR |
Genious 2X SYBR Green Fast qPCR Mix |
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| RK21204 | Abclonal | 1 mL | 76.3 EUR |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
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| 17278 | AAT Bioquest | 1 mL | 203 EUR |
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Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
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| 17278-1mL | AAT Bioquest | 1 mL | 99 EUR |
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Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
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| 17279 | AAT Bioquest | 5 mL | 508 EUR |
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Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
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| 17279-5mL | AAT Bioquest | 5 mL | 308 EUR |
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Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
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| 17280 | AAT Bioquest | 1 mL | 203 EUR |
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Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
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| 17280-1mL | AAT Bioquest | 1 mL | 99 EUR |
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Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
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| 17281 | AAT Bioquest | 5 mL | 508 EUR |
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Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
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| 17281-5mL | AAT Bioquest | 5 mL | 308 EUR |
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Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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2X Universal SYBR Green Fast qPCR Mix |
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| MBS9141672-25mL | MyBiosource | 25mL | 755 EUR |
2X Universal SYBR Green Fast qPCR Mix |
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| MBS9141672-5mL | MyBiosource | 5mL | 255 EUR |
2X Universal SYBR Green Fast qPCR Mix |
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| RK21203 | Abclonal | 1 mL | 21.26 EUR |
2 x SYBR Green qPCR Mix(Antibody，ROX) |
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| 4302A | Unibiotest | 1 mL | 80 EUR |
2 x SYBR Green qPCR Mix(Antibody，ROX) |
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| 4302B | Unibiotest | 5*1 mL | 250 EUR |
2 x SYBR Green qPCR Mix(Antibody，ROX) |
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| 4302C | Unibiotest | 50*1 mL | 1400 EUR |
A cell-free, mate-paired library offered single DNA molecules that have been amplified in parallel to 1-micrometer beads by emulsion polymerase chain response. Millions of beads have been immobilized in a polyacrylamide gel and subjected to automated cycles of sequencing by ligation and four-color imaging. Cost per base was roughly one-ninth as a lot as that of standard sequencing. Our protocols have been carried out with off-the-shelf instrumentation and reagents.