Emerging strategies for the correct quantification of gene expression in particular person cells maintain promise for revealing the extent, operate and origins of cell-to-cell variability. Different high-throughput strategies for single-cell RNA-seq have been launched that fluctuate in protection, sensitivity and multiplexing capability. We lately launched Smart-seq for transcriptome evaluation from single cells, and we subsequently optimized the tactic for improved sensitivity, accuracy and full-length protection throughout transcripts.
Here we current an in depth protocol for Smart-seq2 that enables the era of full-length cDNA and sequencing libraries by using commonplace reagents. The whole protocol takes ∼2 d from cell selecting to having a remaining library prepared for sequencing; sequencing would require an extra 1-Three d relying on the technique and sequencer.
The present limitations are the dearth of strand specificity and the lack to detect nonpolyadenylated (polyA(-)) RNA. Genome modifying pushed by zinc-finger nucleases (ZFNs) yields excessive gene-modification efficiencies (>10%) by introducing a recombinogenic double-strand break into the focused gene. The cleavage occasion is induced using two custom-designed ZFNs that heterodimerize upon binding DNA to type a catalytically lively nuclease advanced. Using the present ZFN structure, nonetheless, cleavage-competent homodimers can also type that may restrict security or efficacy through off-target cleavage.
Here we develop an improved ZFN structure that eliminates this downside. Using structure-based design, we engineer two variant ZFNs that effectively cleave DNA solely when paired as a heterodimer. These ZFNs modify a local endogenous locus as effectively because the parental structure, however with a >40-fold discount in homodimer operate and far decrease ranges of genome-wide cleavage. This structure offers a common means for bettering the specificity of ZFNs as gene modification reagents.
Hyperthermophilic enzymes: sources, makes use of, and molecular mechanisms for thermostability.
Enzymes synthesized by hyperthermophiles (micro organism and archaea with optimum progress temperatures of>> 80 levels C), additionally referred to as hyperthermophilic enzymes, are sometimes thermostable (i.e., immune to irreversible inactivation at excessive temperatures) and are optimally lively at excessive temperatures.
These enzymes share the identical catalytic mechanisms with their mesophilic counterparts. When cloned and expressed in mesophilic hosts, hyperthermophilic enzymes often retain their thermal properties, indicating that these properties are genetically encoded. Sequence alignments, amino acid content material comparisons, crystal construction comparisons, and mutagenesis experiments point out that hyperthermophilic enzymes are, certainly, similar to their mesophilic homologues.
No single mechanism is liable for the exceptional stability of hyperthermophilic enzymes. Increased thermostability should be discovered, as an alternative, in a small variety of extremely particular alterations that usually don’t obey any apparent visitors guidelines. After briefly discussing the range of hyperthermophilic organisms, this evaluate concentrates on the exceptional thermostability of their enzymes.
The biochemical and molecular properties of hyperthermophilic enzymes are described. Mechanisms liable for protein inactivation are reviewed. The molecular mechanisms concerned in protein thermostabilization are mentioned, together with ion pairs, hydrogen bonds, hydrophobic interactions, disulfide bridges, packing, lower of the entropy of unfolding, and intersubunit interactions. Finally, present makes use of and potential functions of thermophilic and hyperthermophilic enzymes as analysis reagents and as catalysts for industrial processes are described.
Nuclear protein import in permeabilized mammalian cells requires soluble cytoplasmic components.
We have developed an in vitro system involving digitonin-permeabilized vertebrate cells to review biochemical occasions within the transport of macromolecules throughout the nuclear envelope. While therapy of cultured cells with digitonin permeabilizes the plasma membranes to macromolecules, the nuclear envelopes stay structurally intact and nuclei retain the power to move and accumulate proteins containing the SV40 giant T antigen nuclear location sequence.
Transport requires addition of exogenous cytosol to permeabilized cells, indicating the soluble cytoplasmic issue(s) required for nuclear import are launched throughout digitonin therapy. In this reconstituted import system, a protein containing a nuclear location sign is quickly amassed in nuclei, the place it reaches a 30-fold focus in comparison with the encircling medium inside 30 min. Nuclear import is particular for a purposeful nuclear location sequence, requires ATP and cytosol, and is temperature dependent.
Furthermore, accumulation of the transport substrate inside nuclei is totally inhibited by wheat germ agglutinin, which binds to nuclear pore complexes and inhibits transport in vivo. Together, these outcomes point out that the permeabilized cell system reproduces genuine nuclear protein import. In a preliminary biochemical dissection of the system, we observe that the sulfhydryl alkylating reagent N-ethylmaleimide inactivates each cytosolic issue(s) and in addition part(s) within the insoluble permeabilized cell fraction required for nuclear protein import.
Because this permeabilized cell mannequin is straightforward, environment friendly, and works successfully with cells and cytosol fractions ready from a wide range of completely different vertebrate sources, it can show highly effective for investigating the biochemical pathway of nuclear transport.
Accurate multiplex polony sequencing of an developed bacterial genome.
We describe a DNA sequencing expertise wherein a generally out there, cheap epifluorescence microscope is transformed to speedy nonelectrophoretic DNA sequencing automation. We apply this expertise to resequence an developed pressure of Escherichia coli at lower than one error per million consensus bases.
AceQ qPCR SYBR® Green Master Mix |
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Q111-02 | Vazyme | 500 rxn (20 μl/rxn) | 265.2 EUR |
AceQ qPCR SYBR® Green Master Mix |
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Q111-03 | Vazyme | 2500 rxn (20 μl/rxn) | 775.2 EUR |
2x SYBR Green qPCR Master Mix (Low ROX) |
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B21702 | Bimake | 5 ml | 268.8 EUR |
Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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2x SYBR Green qPCR Master Mix (Low ROX) |
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B21703 | Bimake | 25 ml | 1027.2 EUR |
Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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2x SYBR Green qPCR Master Mix (High ROX) |
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B21402 | Bimake | 5 ml | 268.8 EUR |
Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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2x SYBR Green qPCR Master Mix (High ROX) |
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B21403 | Bimake | 25 ml | 1027.2 EUR |
Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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AceQ Universal SYBR qPCR Master Mix |
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Q511-02 | Vazyme | 500 rxn (20 μl/rxn) | 265.2 EUR |
AceQ Universal SYBR qPCR Master Mix |
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Q511-03 | Vazyme | 2500 rxn (20 μl/rxn) | 775.2 EUR |
ChamQ Universal SYBR qPCR Master Mix |
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Q711-02 | Vazyme | 500 rxn (20 μl/rxn) | 265.2 EUR |
ChamQ Universal SYBR qPCR Master Mix |
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Q711-03 | Vazyme | 2500 rxn (20 μl/rxn) | 775.2 EUR |
miRNA Universal SYBR® qPCR Master Mix |
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MQ101-01 | Vazyme | 125 rxn(20 μl/rxn) | 165.6 EUR |
miRNA Universal SYBR® qPCR Master Mix |
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MQ101-02 | Vazyme | 500 rxn(20 μl/rxn) | 290.4 EUR |
Genious 2X SYBR Green Fast qPCR Mix |
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RK21204 | Abclonal | 1 mL | Ask for price |
2X Universal SYBR Green Fast qPCR Mix |
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RK21203 | Abclonal | 1 mL | Ask for price |
TAQuest™ qPCR Master Mix with Helixyte™ Green *No ROX* |
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17270 | AAT Bioquest | 1 mL | 99 EUR |
TAQuest™ qPCR Master Mix with Helixyte™ Green *No ROX* |
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17271 | AAT Bioquest | 5 mL | 308 EUR |
TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX* |
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17272 | AAT Bioquest | 1 mL | 99 EUR |
TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX* |
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17273 | AAT Bioquest | 5 mL | 308 EUR |
TAQuest™ qPCR Master Mix with Helixyte™ Green *High ROX* |
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17274 | AAT Bioquest | 1 mL | 99 EUR |
TAQuest™ qPCR Master Mix with Helixyte™ Green *High ROX* |
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17275 | AAT Bioquest | 5 mL | 308 EUR |
lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
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M-PCR-173L | MiTeGen | 960 reactions x 20 µl | 897 EUR |
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
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lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
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M-PCR-173S | MiTeGen | 192 reactions x 20 µl | 224 EUR |
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
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Genious 2X SYBR GREEN FAST qPCR MIX (No ROX) |
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RK21205 | Abclonal | 1 ml | Ask for price |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX* |
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17276 | AAT Bioquest | 1 mL | 99 EUR |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX* |
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17277 | AAT Bioquest | 5 mL | 308 EUR |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX* |
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17278 | AAT Bioquest | 1 mL | 99 EUR |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX* |
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17279 | AAT Bioquest | 5 mL | 308 EUR |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *High ROX* |
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17280 | AAT Bioquest | 1 mL | 99 EUR |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *High ROX* |
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17281 | AAT Bioquest | 5 mL | 308 EUR |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
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P0346-001 | GenDepot | 1ml | 336 EUR |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
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P0346-002 | GenDepot | 2x1ml | 578.4 EUR |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
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P0346-005 | GenDepot | 5x1ml | 993.6 EUR |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
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P0346-010 | GenDepot | 10x1ml | 1632 EUR |
RT Master Mix for qPCR |
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HY-K0510 | MedChemExpress | 1 mL (100 rxns) | 349.2 EUR |
AceQ qPCR Probe Master Mix |
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Q112-02 | Vazyme | 500 rxn (20 μl/rxn) | 265.2 EUR |
AceQ qPCR Probe Master Mix |
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Q112-03 | Vazyme | 2500 rxn (20 μl/rxn) | 775.2 EUR |
HotStart PCR Master mix (2X, Green Dye) |
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BS9291 | Bio Basic | 5X1mL, 5ml | 251.4 EUR |
HotStart PCR Master mix (2X, Green Dye) |
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BS9292 | Bio Basic | 1ml | 115.68 EUR |
Entrans 2X qPCR Probe Master Mix |
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RK21208 | Abclonal | 40 RXN | Ask for price |
Fast Plus EvaGreen qPCR Master Mix (5000 rxn) |
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31020-2 | Biotium | 50x1mL | 4291.2 EUR |
Description: Minimum order quantity: 1 unit of 50x1mL |
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Fast Plus EvaGreen qPCR Master Mix (500 rxn) |
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31020-1 | Biotium | 5x1mL | 524.4 EUR |
Description: Minimum order quantity: 1 unit of 5x1mL |
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Fast EvaGreen Master Mix for qPCR (5000 rxn) |
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31003-2 | Biotium | 50x1ML | 3739.2 EUR |
Description: Minimum order quantity: 1 unit of 50x1ML |
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Fast EvaGreen Master Mix for qPCR(200 rxn) |
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31003 | Biotium | 2x1ML | 244.8 EUR |
Description: Minimum order quantity: 1 unit of 2x1ML |
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Fast EvaGreen Master Mix for qPCR (500 rxn) |
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31003-1 | Biotium | 5x1ML | 463.2 EUR |
Description: Minimum order quantity: 1 unit of 5x1ML |
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Green-2-Go qPCR Mastermix (500X20ul Rxn) |
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QPCR004-S | Bio Basic | 4X1.25ml | 303.25 EUR |
RT Master Mix for qPCR (gDNA digester plus) |
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HY-K0511 | MedChemExpress | 1 mL (100 rxns) | 378 EUR |
Genorise® 2 x qPCR HotStart Master Mix |
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GR108002 | Genorise Scientific | 2.0 ml | 279 EUR |
Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (2000 reactions) |
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31041-20mL | Biotium | 2x10mL | 592.8 EUR |
Description: Minimum order quantity: 1 unit of 2x10mL |
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (500 reactions) |
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31041-1 | Biotium | 5x1mL | 326.4 EUR |
Description: Minimum order quantity: 1 unit of 5x1mL |
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (100 reactions) |
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31041-T | Biotium | 1mL | 136.8 EUR |
Description: Minimum order quantity: 1 unit of 1mL |
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Green-2-Go qPCR Mastermix-ROX (500X20ul Rxn) |
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QPCR001-R | Bio Basic | 4X1.25ml | 303.25 EUR |
TAQuest™ qPCR Master Mix for TaqMan Probes *No ROX* |
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17282 | AAT Bioquest | 1 mL | 99 EUR |
TAQuest™ qPCR Master Mix for TaqMan Probes *No ROX* |
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17283 | AAT Bioquest | 5 mL | 308 EUR |
TAQuest™ qPCR Master Mix for TaqMan Probes *Low ROX* |
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17284 | AAT Bioquest | 1 mL | 99 EUR |
TAQuest™ qPCR Master Mix for TaqMan Probes *Low ROX* |
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17285 | AAT Bioquest | 5 mL | 308 EUR |
Fast Plus EvaGreen qPCR Master Mix (trial size, 100 rxn) |
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31020-T | Biotium | 1mL | 160.8 EUR |
Description: Minimum order quantity: 1 unit of 1mL |
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Green-2-Go qPCR Mastermix-iCycler (500X20ul Rxn) |
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QPCR003-iC | Bio Basic | 4X1.25ml | 303.25 EUR |
TAQuest™ qPCR Master Mix for TaqMan Probes *High ROX* |
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17286 | AAT Bioquest | 1 mL | 99 EUR |
TAQuest™ qPCR Master Mix for TaqMan Probes *High ROX* |
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17287 | AAT Bioquest | 5 mL | 308 EUR |
Green-2-Go qPCR Mastermix-no Dye (500X20ul Rxn) |
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QPCR006-NODYE | Bio Basic | 4X1.25ml | 326.22 EUR |
Green-2-Go qPCR Mastermix-Low ROX (500X20ul Rxn) |
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QPCR002-L | Bio Basic | 4X1.25ml | 303.25 EUR |
Fast Plus EvaGreen qPCR Master Mix with low Rox (500 rxn) |
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31014-1 | Biotium | 5x1mL | 524.4 EUR |
Description: Minimum order quantity: 1 unit of 5x1mL |
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Fast Plus EvaGreen qPCR Master Mix with low Rox (5000 rxn) |
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31014-2 | Biotium | 50x1mL | 4291.2 EUR |
Description: Minimum order quantity: 1 unit of 50x1mL |
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Fast Plus EvaGreen qPCR Master Mix with high Rox (500 rxn) |
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31015-1 | Biotium | 5x1mL | 524.4 EUR |
Description: Minimum order quantity: 1 unit of 5x1mL |
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Fast Plus EvaGreen qPCR Master Mix with high Rox (5000 rxn) |
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31015-2 | Biotium | 50x1mL | 4291.2 EUR |
Description: Minimum order quantity: 1 unit of 50x1mL |
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Fast EvaGreen Master Mix for qPCR, trial size (100 rxn) |
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31003-T | Biotium | 1ML | 147.6 EUR |
Description: Minimum order quantity: 1 unit of 1ML |
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TAQuest™ FAST qPCR Master Mix for TaqMan Probes *No ROX* |
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17288 | AAT Bioquest | 1 mL | 99 EUR |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *No ROX* |
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17289 | AAT Bioquest | 5 mL | 308 EUR |
qPCR Master Mix DLP1 (SafeLine: dUTP), packed in 1,25 ml vials |
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S190 | GeneOn | 100 rcs (2,5 ml) | 165.6 EUR |
qPCR Master Mix DLP1 (SafeLine: dUTP), packed in 1,25 ml vials |
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S190L | GeneOn | 500 rcs (12,5 ml) | 627.6 EUR |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX* |
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17290 | AAT Bioquest | 1 mL | 99 EUR |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX* |
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17291 | AAT Bioquest | 5 mL | 308 EUR |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *High ROX* |
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17292 | AAT Bioquest | 1 mL | 99 EUR |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *High ROX* |
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17293 | AAT Bioquest | 5 mL | 308 EUR |
SYBR Green I |
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B1747-25 | Biovision | each | 1396.8 EUR |
SYBR Green I |
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B1747-5 | Biovision | each | 464.4 EUR |
Green-2-Go TaqProbe qPCR Mastermix-no Dye (500X20ul Rxn) |
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QPCR005-NODYE | Bio Basic | 4X1.25ml | 303.25 EUR |
qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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M-PCR-372L | MiTeGen | 10 x 1,25 ml | 818 EUR |
Description: qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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M-PCR-372S | MiTeGen | 2 x 1,25 ml | 205 EUR |
Description: qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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Fast Plus EvaGreen qPCR Master Mix with low Rox (100 rxn): (1mL) |
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31014-T | Biotium | 1mL | 160.8 EUR |
Description: Minimum order quantity: 1 unit of 1mL |
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (Low ROX) (500 reactions) |
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9-31045 | Biotium |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (500 reactions) |
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31042-1 | Biotium | 5x1mL | 326.4 EUR |
Description: Minimum order quantity: 1 unit of 5x1mL |
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (200 reactions) |
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31042-20mL | Biotium | 2x10mL | 592.8 EUR |
Description: Minimum order quantity: 1 unit of 2x10mL |
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (100 reactions) |
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31042-T | Biotium | 1mL | 136.8 EUR |
Description: Minimum order quantity: 1 unit of 1mL |
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (High ROX) (500 reactions) |
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9-31046 | Biotium |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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Fast Plus EvaGreen qPCR Master Mix with High Rox (100 rxn): (1mL) |
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31015-T | Biotium | 1mL | 160.8 EUR |
Description: Minimum order quantity: 1 unit of 1mL |
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Fast Plus EvaGreen Master Mix for qPCR with Rox (200 rxn) |
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31014 | Biotium | 2x1mL | 273.6 EUR |
Description: Minimum order quantity: 1 unit of 2x1mL |
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qPCR Master Mix DLP2 (SafeLine: dUTP, UNG), packed in 1,25 ml vials |
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S200 | GeneOn | 100 rcs (2,5 ml) | 190.8 EUR |
qPCR Master Mix DLP2 (SafeLine: dUTP, UNG), packed in 1,25 ml vials |
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S200L | GeneOn | 500 rcs (12,5 ml) | 734.4 EUR |
CRISPY™ Master Mix for qPCR-based Gene Editing Quantitation |
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CRISPY100A-1 | SBI | 200 rxn | 412 EUR |
qPCR Master Mix E1 (SafeLine: EvaGreen, dUTP), packed in 1,25 ml vials |
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S150 | GeneOn | 100 rcs (2,5 ml) | 165.6 EUR |
qPCR Master Mix E1 (SafeLine: EvaGreen, dUTP), packed in 1,25 ml vials |
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S150L | GeneOn | 500 rcs (12,5 ml) | 627.6 EUR |
qPCR SybrMaster lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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M-PCR-373L | MiTeGen | 10 x 1,25 ml | 818 EUR |
Description: qPCR SybrMaster lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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qPCR SybrMaster lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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M-PCR-373S | MiTeGen | 2 x 1,25 ml | 205 EUR |
Description: qPCR SybrMaster lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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qPCR SybrMaster UNGMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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M-PCR-375L | MiTeGen | 10 x 1,25 ml | 982 EUR |
Description: qPCR SybrMaster UNGMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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qPCR SybrMaster UNGMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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M-PCR-375S | MiTeGen | 2 x 1,25 ml | 246 EUR |
Description: qPCR SybrMaster UNGMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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qPCR SybrMaster highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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M-PCR-374L | MiTeGen | 10 x 1,25 ml | 818 EUR |
Description: qPCR SybrMaster highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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qPCR SybrMaster highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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M-PCR-374S | MiTeGen | 2 x 1,25 ml | 205 EUR |
Description: qPCR SybrMaster highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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Fast Plus EvaGreen Master Mix for qPCR with High Rox (200 rxn) |
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31015 | Biotium | 2x1mL | 273.6 EUR |
Description: Minimum order quantity: 1 unit of 2x1mL |
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qPCR Master Mix E2 (SafeLine: EvaGreen, dUTP, UNG), packed in 1,25 ml vials |
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S160 | GeneOn | 100 rcs (2,5 ml) | 190.8 EUR |
qPCR Master Mix E2 (SafeLine: EvaGreen, dUTP, UNG), packed in 1,25 ml vials |
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S160L | GeneOn | 500 rcs (12,5 ml) | 734.4 EUR |
ABScript II One Step SYBR Green RT-qPCR Kit |
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RK20404 | Abclonal | 10 RXN | Ask for price |
qPCR SybrMaster UNG lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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M-PCR-376L | MiTeGen | 10 x 1,25 ml | 982 EUR |
Description: qPCR SybrMaster UNG lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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qPCR SybrMaster UNG lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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M-PCR-376S | MiTeGen | 2 x 1,25 ml | 246 EUR |
Description: qPCR SybrMaster UNG lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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qPCR SybrMaster UNG highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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M-PCR-377L | MiTeGen | 10 x 1,25 ml | 982 EUR |
Description: qPCR SybrMaster UNG highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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qPCR SybrMaster UNG highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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M-PCR-377S | MiTeGen | 2 x 1,25 ml | 246 EUR |
Description: qPCR SybrMaster UNG highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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qPCR Master Mix DLP3 (SafeLine: dUTP, 500 nM ROX), packed in 1,25 ml vials |
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S210 | GeneOn | 100 rcs (2,5 ml) | 165.6 EUR |
qPCR Master Mix DLP3 (SafeLine: dUTP, 500 nM ROX), packed in 1,25 ml vials |
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S210L | GeneOn | 500 rcs (12,5 ml) | 627.6 EUR |
LncRNA Profiler Complete qPCR Array Kit (cDNA synthesis kit, qPCR array and SYBR Green reagent) 20 profiles |
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RA910A-1 | SBI | 20 profiles | 1329.6 EUR |
EZSolution? SYBR Green I |
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B1748-1 | Biovision | each | 744 EUR |
EZSolution? SYBR Green I |
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B1748-500 | Biovision | each | 516 EUR |
CytoTrace™ Ultra Green |
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21800 | AAT Bioquest | 1 mg | 278 EUR |
qPCR Master Mix E5 (EvaGreen, dUTP, 100 nM LOW ROX), packed in 1,25 ml vials |
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S175 | GeneOn | 100 rcs (2,5 ml) | 165.6 EUR |
qPCR Master Mix E5 (EvaGreen, dUTP, 100 nM LOW ROX), packed in 1,25 ml vials |
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S175L | GeneOn | 500 rcs (12,5 ml) | 627.6 EUR |
qPCR Master Mix DLP5 (SafeLine: dUTP, 100 nM LOW ROX), packed in 1,25 ml vials |
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S215 | GeneOn | 100 rcs (2,5 ml) | 190.8 EUR |
qPCR Master Mix DLP5 (SafeLine: dUTP, 100 nM LOW ROX), packed in 1,25 ml vials |
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S215L | GeneOn | 500 rcs (12,5 ml) | 627.6 EUR |
qPCR Master Mix DLP4 (SafeLine: dUTP, UNG, 500 nM ROX), packed in 1,25 ml vials |
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S220 | GeneOn | 100 rcs (2,5 ml) | 190.8 EUR |
qPCR Master Mix DLP4 (SafeLine: dUTP, UNG, 500 nM ROX), packed in 1,25 ml vials |
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S220L | GeneOn | 500 rcs (12,5 ml) | 734.4 EUR |
qPCR Master Mix DLP6 (SafeLine: dUTP, UNG, 100 nM ROX), packed in 1,25 ml vials |
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S225 | GeneOn | 100 rcs (2,5 ml) | 190.8 EUR |
qPCR Master Mix DLP6 (SafeLine: dUTP, UNG, 100 nM ROX), packed in 1,25 ml vials |
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S225L | GeneOn | 500 rcs (12,5 ml) | 734.4 EUR |
Jade? Master Mix |
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M1105-500 | Biovision | each | 529.2 EUR |
Green Dye One Step qRT-PCR Master Mix For Quantitative Real Time PCR With ROX Dye |
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MB1004-100Reactions | BosterBio | 100 Reactions | 392.4 EUR |
Taqman Master Mix |
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M1121-500 | Biovision | each | 529.2 EUR |
qPCR Master Mix E3 (SafeLine: EvaGreen, dUTP, 500 nM ROX), packed in 1,25 ml vials |
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S170 | GeneOn | 100 rcs (2,5 ml) | 165.6 EUR |
qPCR Master Mix E3 (SafeLine: EvaGreen, dUTP, 500 nM ROX), packed in 1,25 ml vials |
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S170L | GeneOn | 500 rcs (12,5 ml) | 627.6 EUR |
qPCR Master Mix E6 (EvaGreen, dUTP, UNG, 100 nM LOW ROX), packed in 1,25 ml vials |
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S185 | GeneOn | 100 rcs (2,5 ml) | 190.8 EUR |
qPCR Master Mix E6 (EvaGreen, dUTP, UNG, 100 nM LOW ROX), packed in 1,25 ml vials |
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S185L | GeneOn | 500 rcs (12,5 ml) | 734.4 EUR |
Accuris qMax Green, No Rox qPCR Mix, 1000 reactions |
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PR2000-N-1000 | Benchmark Scientific | 1 PC | 623.8 EUR |
amfiSure qGreen Q-PCR Master Mix(2X), No Rox |
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Q5600-001 | GenDepot | 1ml | 175.2 EUR |
amfiSure qGreen Q-PCR Master Mix(2X), No Rox |
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Q5600-002 | GenDepot | 2x1ml | 248.4 EUR |
amfiSure qGreen Q-PCR Master Mix(2X), No Rox |
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Q5600-005 | GenDepot | 5x1ml | 322.8 EUR |
amfiSure qGreen Q-PCR Master Mix(2X), No Rox |
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Q5600-010 | GenDepot | 10x1ml | 537.6 EUR |
amfiSure qGreen Q-PCR Master Mix(2X), No Rox |
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Q5600-050 | GenDepot | 50x1ml | 2085.6 EUR |
amfiSure qProbe Q-PCR Master Mix(2X), No Rox |
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Q5700-001 | GenDepot | 1ml | 208.8 EUR |
amfiSure qProbe Q-PCR Master Mix(2X), No Rox |
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Q5700-002 | GenDepot | 2x1ml | 319.2 EUR |
amfiSure qProbe Q-PCR Master Mix(2X), No Rox |
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Q5700-005 | GenDepot | 5x1ml | 562.8 EUR |
amfiSure qProbe Q-PCR Master Mix(2X), No Rox |
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Q5700-010 | GenDepot | 10x1ml | 886.8 EUR |
amfiSure qProbe Q-PCR Master Mix(2X), No Rox |
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Q5700-050 | GenDepot | 50x1ml | 3462 EUR |
amfiSure qGreen Q-PCR Master Mix(2X), Fluorescein |
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Q5601-001 | GenDepot | 1ml | 175.2 EUR |
amfiSure qGreen Q-PCR Master Mix(2X), Fluorescein |
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Q5601-002 | GenDepot | 2x1ml | 248.4 EUR |
amfiSure qGreen Q-PCR Master Mix(2X), Fluorescein |
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Q5601-005 | GenDepot | 5x1ml | 319.2 EUR |
amfiSure qGreen Q-PCR Master Mix(2X), Fluorescein |
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Q5601-010 | GenDepot | 10x1ml | 530.4 EUR |
amfiSure qGreen Q-PCR Master Mix(2X), Fluorescein |
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Q5601-050 | GenDepot | 50x1ml | 2004 EUR |
amfiSure qProbe Q-PCR Master Mix(2X), Fluorescein |
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Q5701-001 | GenDepot | 1ml | 208.8 EUR |
amfiSure qProbe Q-PCR Master Mix(2X), Fluorescein |
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Q5701-002 | GenDepot | 2x1ml | 319.2 EUR |
amfiSure qProbe Q-PCR Master Mix(2X), Fluorescein |
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Q5701-005 | GenDepot | 5x1ml | 562.8 EUR |
amfiSure qProbe Q-PCR Master Mix(2X), Fluorescein |
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Q5701-010 | GenDepot | 10x1ml | 886.8 EUR |
amfiSure qProbe Q-PCR Master Mix(2X), Fluorescein |
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Q5701-050 | GenDepot | 50x1ml | 3462 EUR |
Accuris qMax Green, Low Rox qPCR Mix, 1000 reactions |
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PR2000-L-1000 | Benchmark Scientific | 1 PC | 623.8 EUR |
amfiSure qGreen Q-PCR Master Mix(2X), Low Rox |
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Q5603-001 | GenDepot | 1ml | 175.2 EUR |
amfiSure qGreen Q-PCR Master Mix(2X), Low Rox |
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Q5603-002 | GenDepot | 2x1ml | 248.4 EUR |
amfiSure qGreen Q-PCR Master Mix(2X), Low Rox |
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Q5603-005 | GenDepot | 5x1ml | 319.2 EUR |
amfiSure qGreen Q-PCR Master Mix(2X), Low Rox |
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Q5603-010 | GenDepot | 10x1ml | 530.4 EUR |
amfiSure qGreen Q-PCR Master Mix(2X), Low Rox |
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Q5603-050 | GenDepot | 50x1ml | 2004 EUR |
amfiSure qProbe Q-PCR Master Mix(2X), Low Rox |
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Q5703-001 | GenDepot | 1ml | 208.8 EUR |
amfiSure qProbe Q-PCR Master Mix(2X), Low Rox |
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Q5703-002 | GenDepot | 2x1ml | 319.2 EUR |
amfiSure qProbe Q-PCR Master Mix(2X), Low Rox |
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Q5703-003 | GenDepot | 5x1ml | 562.8 EUR |
amfiSure qProbe Q-PCR Master Mix(2X), Low Rox |
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Q5703-004 | GenDepot | 10x1ml | 886.8 EUR |
amfiSure qProbe Q-PCR Master Mix(2X), Low Rox |
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Q5703-005 | GenDepot | 50x1ml | 3462 EUR |
Accuris qMax Green, High Rox qPCR Mix, 1000 reactions |
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PR2000-H-1000 | Benchmark Scientific | 1 PC | 623.8 EUR |
amfiSure qGreen Q-PCR Master Mix(2X), High Rox |
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Q5602-001 | GenDepot | 1ml | 175.2 EUR |
amfiSure qGreen Q-PCR Master Mix(2X), High Rox |
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Q5602-002 | GenDepot | 2x1ml | 248.4 EUR |
amfiSure qGreen Q-PCR Master Mix(2X), High Rox |
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Q5602-005 | GenDepot | 5x1ml | 319.2 EUR |
amfiSure qGreen Q-PCR Master Mix(2X), High Rox |
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Q5602-010 | GenDepot | 10x1ml | 530.4 EUR |
amfiSure qGreen Q-PCR Master Mix(2X), High Rox |
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Q5602-050 | GenDepot | 50x1ml | 2004 EUR |
amfiSure qProbe Q-PCR Master Mix(2X), High Rox |
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Q5702-001 | GenDepot | 1ml | 208.8 EUR |
A cell-free, mate-paired library offered single DNA molecules that have been amplified in parallel to 1-micrometer beads by emulsion polymerase chain response. Millions of beads have been immobilized in a polyacrylamide gel and subjected to automated cycles of sequencing by ligation and four-color imaging. Cost per base was roughly one-ninth as a lot as that of standard sequencing. Our protocols have been carried out with off-the-shelf instrumentation and reagents.