Emerging strategies for the correct quantification of gene expression in particular person cells maintain promise for revealing the extent, operate and origins of cell-to-cell variability. Different high-throughput strategies for single-cell RNA-seq have been launched that fluctuate in protection, sensitivity and multiplexing capability. We lately launched Smart-seq for transcriptome evaluation from single cells, and we subsequently optimized the tactic for improved sensitivity, accuracy and full-length protection throughout transcripts.
Here we current an in depth protocol for Smart-seq2 that enables the era of full-length cDNA and sequencing libraries by using commonplace reagents. The whole protocol takes ∼2 d from cell selecting to having a remaining library prepared for sequencing; sequencing would require an extra 1-Three d relying on the technique and sequencer.
The present limitations are the dearth of strand specificity and the lack to detect nonpolyadenylated (polyA(-)) RNA. Genome modifying pushed by zinc-finger nucleases (ZFNs) yields excessive gene-modification efficiencies (>10%) by introducing a recombinogenic double-strand break into the focused gene. The cleavage occasion is induced using two custom-designed ZFNs that heterodimerize upon binding DNA to type a catalytically lively nuclease advanced. Using the present ZFN structure, nonetheless, cleavage-competent homodimers can also type that may restrict security or efficacy through off-target cleavage.
Here we develop an improved ZFN structure that eliminates this downside. Using structure-based design, we engineer two variant ZFNs that effectively cleave DNA solely when paired as a heterodimer. These ZFNs modify a local endogenous locus as effectively because the parental structure, however with a >40-fold discount in homodimer operate and far decrease ranges of genome-wide cleavage. This structure offers a common means for bettering the specificity of ZFNs as gene modification reagents.
Hyperthermophilic enzymes: sources, makes use of, and molecular mechanisms for thermostability.
Enzymes synthesized by hyperthermophiles (micro organism and archaea with optimum progress temperatures of>> 80 levels C), additionally referred to as hyperthermophilic enzymes, are sometimes thermostable (i.e., immune to irreversible inactivation at excessive temperatures) and are optimally lively at excessive temperatures.
These enzymes share the identical catalytic mechanisms with their mesophilic counterparts. When cloned and expressed in mesophilic hosts, hyperthermophilic enzymes often retain their thermal properties, indicating that these properties are genetically encoded. Sequence alignments, amino acid content material comparisons, crystal construction comparisons, and mutagenesis experiments point out that hyperthermophilic enzymes are, certainly, similar to their mesophilic homologues.
No single mechanism is liable for the exceptional stability of hyperthermophilic enzymes. Increased thermostability should be discovered, as an alternative, in a small variety of extremely particular alterations that usually don’t obey any apparent visitors guidelines. After briefly discussing the range of hyperthermophilic organisms, this evaluate concentrates on the exceptional thermostability of their enzymes.
The biochemical and molecular properties of hyperthermophilic enzymes are described. Mechanisms liable for protein inactivation are reviewed. The molecular mechanisms concerned in protein thermostabilization are mentioned, together with ion pairs, hydrogen bonds, hydrophobic interactions, disulfide bridges, packing, lower of the entropy of unfolding, and intersubunit interactions. Finally, present makes use of and potential functions of thermophilic and hyperthermophilic enzymes as analysis reagents and as catalysts for industrial processes are described.
Nuclear protein import in permeabilized mammalian cells requires soluble cytoplasmic components.
We have developed an in vitro system involving digitonin-permeabilized vertebrate cells to review biochemical occasions within the transport of macromolecules throughout the nuclear envelope. While therapy of cultured cells with digitonin permeabilizes the plasma membranes to macromolecules, the nuclear envelopes stay structurally intact and nuclei retain the power to move and accumulate proteins containing the SV40 giant T antigen nuclear location sequence.
Transport requires addition of exogenous cytosol to permeabilized cells, indicating the soluble cytoplasmic issue(s) required for nuclear import are launched throughout digitonin therapy. In this reconstituted import system, a protein containing a nuclear location sign is quickly amassed in nuclei, the place it reaches a 30-fold focus in comparison with the encircling medium inside 30 min. Nuclear import is particular for a purposeful nuclear location sequence, requires ATP and cytosol, and is temperature dependent.
Furthermore, accumulation of the transport substrate inside nuclei is totally inhibited by wheat germ agglutinin, which binds to nuclear pore complexes and inhibits transport in vivo. Together, these outcomes point out that the permeabilized cell system reproduces genuine nuclear protein import. In a preliminary biochemical dissection of the system, we observe that the sulfhydryl alkylating reagent N-ethylmaleimide inactivates each cytosolic issue(s) and in addition part(s) within the insoluble permeabilized cell fraction required for nuclear protein import.
Because this permeabilized cell mannequin is straightforward, environment friendly, and works successfully with cells and cytosol fractions ready from a wide range of completely different vertebrate sources, it can show highly effective for investigating the biochemical pathway of nuclear transport.
Accurate multiplex polony sequencing of an developed bacterial genome.
We describe a DNA sequencing expertise wherein a generally out there, cheap epifluorescence microscope is transformed to speedy nonelectrophoretic DNA sequencing automation. We apply this expertise to resequence an developed pressure of Escherichia coli at lower than one error per million consensus bases.
2x SYBR Green qPCR Master Mix (Low ROX) |
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| B21702 | Bimake | 5 ml | 268.8 EUR |
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Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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2x SYBR Green qPCR Master Mix (Low ROX) |
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| B21703 | Bimake | 25 ml | 1027.2 EUR |
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Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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2x SYBR Green qPCR Master Mix (High ROX) |
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| B21402 | Bimake | 5 ml | 268.8 EUR |
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Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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2x SYBR Green qPCR Master Mix (High ROX) |
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| B21403 | Bimake | 25 ml | 1027.2 EUR |
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Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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AceQ qPCR SYBR® Green Master Mix |
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| Q111-02 | Vazyme | 500 rxn (20 μl/rxn) | 265.2 EUR |
AceQ qPCR SYBR® Green Master Mix |
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| Q111-03 | Vazyme | 2500 rxn (20 μl/rxn) | 775.2 EUR |
HotStart™ 2X SYBR Green qPCR Master Mix |
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| K1070-25 | ApexBio | 25ml | 420 EUR |
HotStart™ 2X SYBR Green qPCR Master Mix |
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| K1070-5 | ApexBio | 5ml | 120 EUR |
HotStart™ 2X SYBR Green qPCR Master Mix |
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| K1070-50 | ApexBio | 50ml | 840 EUR |
HotStart™ Universal 2X SYBR Green qPCR Master Mix |
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| K1170-25 | ApexBio | 25ml | 420 EUR |
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Description: qPCR Kit, Universal SYBR Green I Master Mix (Rox) |
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HotStart™ Universal 2X SYBR Green qPCR Master Mix |
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| K1170-5 | ApexBio | 5ml | 120 EUR |
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Description: qPCR Kit, Universal SYBR Green I Master Mix (Rox) |
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HotStart™ Universal 2X SYBR Green qPCR Master Mix |
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| K1170-50 | ApexBio | 50ml | 840 EUR |
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Description: qPCR Kit, Universal SYBR Green I Master Mix (Rox) |
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ChamQ SYBR qPCR Master Mix |
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| Q311-02 | Vazyme | 500 rxns (20 μl/rxn) | 96.44 EUR |
ChamQ SYBR qPCR Master Mix |
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| Q311-03 | Vazyme | 2,500 rxns (20 μl/rxn) | 434.8 EUR |
ChamQ SYBR Color qPCR Master Mix |
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| Q411-02 | Vazyme | 500 rxns (20 μl/rxn) | 112.5 EUR |
ChamQ SYBR Color qPCR Master Mix |
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| Q411-03 | Vazyme | 2,500 rxns (20 μl/rxn) | 507.2 EUR |
AceQ Universal SYBR qPCR Master Mix |
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| Q511-02 | Vazyme | 500 rxn (20 μl/rxn) | 265.2 EUR |
AceQ Universal SYBR qPCR Master Mix |
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| Q511-03 | Vazyme | 2500 rxn (20 μl/rxn) | 775.2 EUR |
ChamQ Universal SYBR qPCR Master Mix |
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| Q711-02 | Vazyme | 500 rxn (20 μl/rxn) | 265.2 EUR |
ChamQ Universal SYBR qPCR Master Mix |
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| Q711-03 | Vazyme | 2500 rxn (20 μl/rxn) | 775.2 EUR |
ChamQ SYBR qPCR Master Mix (Without ROX) |
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| Q321-02 | Vazyme | 500 rxns (20 μl/rxn) | 96.44 EUR |
ChamQ SYBR qPCR Master Mix (Without ROX) |
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| Q321-03 | Vazyme | 2,500 rxns (20 μl/rxn) | 434.8 EUR |
Taq Pro Universal SYBR qPCR Master Mix |
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| Q712-02 | Vazyme | 500 rxns (20 μl/rxn) | 128.8 EUR |
Taq Pro Universal SYBR qPCR Master Mix |
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| Q712-03 | Vazyme | 2,500 rxns (20 μl/rxn) | 580 EUR |
miRNA Universal SYBR® qPCR Master Mix |
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| MQ101-01 | Vazyme | 125 rxn(20 μl/rxn) | 165.6 EUR |
miRNA Universal SYBR® qPCR Master Mix |
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| MQ101-02 | Vazyme | 500 rxn(20 μl/rxn) | 290.4 EUR |
ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
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| Q331-02 | Vazyme | 500 rxns (20 μl/rxn) | 96.44 EUR |
ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
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| Q331-03 | Vazyme | 2,500 rxns (20 μl/rxn) | 434.8 EUR |
ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
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| Q341-02 | Vazyme | 500 rxns (20 μl/rxn) | 96.44 EUR |
ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
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| Q341-03 | Vazyme | 2,500 rxns (20 μl/rxn) | 434.8 EUR |
ChamQ SYBR Color qPCR Master Mix (Without ROX) |
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| Q421-02 | Vazyme | 500 rxns (20 μl/rxn) | 112.5 EUR |
ChamQ SYBR Color qPCR Master Mix (Without ROX) |
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| Q421-03 | Vazyme | 2,500 rxns (20 μl/rxn) | 507.2 EUR |
ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed) |
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| Q431-02 | Vazyme | 500 rxns (20 μl/rxn) | 112.5 EUR |
ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed) |
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| Q431-03 | Vazyme | 2,500 rxns (20 μl/rxn) | 507.2 EUR |
ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) |
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| Q441-02 | Vazyme | 500 rxns (20 μl/rxn) | 112.5 EUR |
ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) |
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| Q441-03 | Vazyme | 2,500 rxns (20 μl/rxn) | 507.2 EUR |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
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| 17270-1mL | AAT Bioquest | 1 mL | 99 EUR |
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Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
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| 17271-5mL | AAT Bioquest | 5 mL | 308 EUR |
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Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
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| 17272-1mL | AAT Bioquest | 1 mL | 99 EUR |
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Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
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| 17273-5mL | AAT Bioquest | 5 mL | 308 EUR |
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Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
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| 17274-1mL | AAT Bioquest | 1 mL | 99 EUR |
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Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
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| 17275-5mL | AAT Bioquest | 5 mL | 308 EUR |
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Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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Genious 2X SYBR Green Fast qPCR Mix |
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| RK21204 | Abclonal | 1 mL | Ask for price |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
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| 17276-1mL | AAT Bioquest | 1 mL | 99 EUR |
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Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
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| 17277-5mL | AAT Bioquest | 5 mL | 308 EUR |
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Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
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| 17278-1mL | AAT Bioquest | 1 mL | 99 EUR |
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Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
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| 17279-5mL | AAT Bioquest | 5 mL | 308 EUR |
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Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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2X Universal SYBR Green Fast qPCR Mix |
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| RK21203 | Abclonal | 1 mL | Ask for price |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
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| 17280-1mL | AAT Bioquest | 1 mL | 99 EUR |
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Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
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| 17281-5mL | AAT Bioquest | 5 mL | 308 EUR |
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Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuest™ qPCR Master Mix with Helixyte™ Green *No ROX* |
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| 17270 | AAT Bioquest | 1 mL | 99 EUR |
TAQuest™ qPCR Master Mix with Helixyte™ Green *No ROX* |
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| 17271 | AAT Bioquest | 5 mL | 308 EUR |
TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX* |
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| 17272 | AAT Bioquest | 1 mL | 99 EUR |
TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX* |
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| 17273 | AAT Bioquest | 5 mL | 308 EUR |
TAQuest™ qPCR Master Mix with Helixyte™ Green *High ROX* |
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| 17274 | AAT Bioquest | 1 mL | 99 EUR |
TAQuest™ qPCR Master Mix with Helixyte™ Green *High ROX* |
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| 17275 | AAT Bioquest | 5 mL | 308 EUR |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX* |
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| 17276 | AAT Bioquest | 1 mL | 99 EUR |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX* |
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| 17277 | AAT Bioquest | 5 mL | 308 EUR |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX* |
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| 17278 | AAT Bioquest | 1 mL | 99 EUR |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX* |
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| 17279 | AAT Bioquest | 5 mL | 308 EUR |
lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
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| M-PCR-173L | MiTeGen | 960 reactions x 20 µl | 897 EUR |
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Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
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lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
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| M-PCR-173S | MiTeGen | 192 reactions x 20 µl | 224 EUR |
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Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
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TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *High ROX* |
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| 17280 | AAT Bioquest | 1 mL | 99 EUR |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *High ROX* |
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| 17281 | AAT Bioquest | 5 mL | 308 EUR |
BrightCycle Universal SYBR Green qPCR Mix with UDG |
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| RK21219 | Abclonal | 5mL | 475 EUR |
Genious 2X SYBR GREEN FAST qPCR MIX (No ROX) |
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| RK21205 | Abclonal | 1 ml | Ask for price |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
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| P0346-001 | GenDepot | 1ml | 336 EUR |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
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| P0346-002 | GenDepot | 2x1ml | 578.4 EUR |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
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| P0346-005 | GenDepot | 5x1ml | 993.6 EUR |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
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| P0346-010 | GenDepot | 10x1ml | 1632 EUR |
RT Master Mix for qPCR |
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| HY-K0510 | MedChemExpress | 1 mL (100 rxns) | 349.2 EUR |
AceQ qPCR Probe Master Mix |
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| Q112-02 | Vazyme | 500 rxn (20 μl/rxn) | 265.2 EUR |
AceQ qPCR Probe Master Mix |
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| Q112-03 | Vazyme | 2500 rxn (20 μl/rxn) | 775.2 EUR |
1-Step 2X RT-PCR Master Mix-SYBR Green-HY (High Yield) |
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| W141 | 101Bio | 200 rxn | 199 EUR |
HotStart PCR Master mix (2X, Green Dye) |
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| BS9291 | Bio Basic | 5X1mL, 5ml | 251.4 EUR |
HotStart PCR Master mix (2X, Green Dye) |
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| BS9292 | Bio Basic | 1ml | 115.68 EUR |
1-Step 2X RT-PCR Master Mix-SYBR Green-HP (High Processibility) |
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| W142 | 101Bio | 200 rxn | 199 EUR |
Entrans 2X qPCR Probe Master Mix |
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| RK21208 | Abclonal | 40 RXN | Ask for price |
Fast Plus EvaGreen qPCR Master Mix (5000 rxn) |
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| 31020-2 | Biotium | 50x1mL | 4291.2 EUR |
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Description: Minimum order quantity: 1 unit of 50x1mL |
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Fast Plus EvaGreen qPCR Master Mix (500 rxn) |
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| 31020-1 | Biotium | 5x1mL | 524.4 EUR |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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EZGreen 2x qPCR Master Mix, 40Rxns-50ul |
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| A4157-1ML40Rxns | Biomatik Corporation | 1ML (40Rxns) | 55 EUR |
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Description: Biotechnology |
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Green-2-Go qPCR Mastermix (500X20ul Rxn) |
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| QPCR004-S | Bio Basic | 4X1.25ml | 303.25 EUR |
ABScript III RT Master Mix for qPCR |
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| RK20428 | Abclonal | 10RXN | 82.5 EUR |
ABScript Neo RT Master Mix for qPCR |
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| RK20432 | Abclonal | 100RXN | 17.5 EUR |
Entrans 2X qPCR Probe Master Mix V2 |
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| RK21212 | Abclonal | 50RXN | 67.5 EUR |
Fast EvaGreen Master Mix for qPCR (5000 rxn) |
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| 31003-2 | Biotium | 50x1ML | 3739.2 EUR |
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Description: Minimum order quantity: 1 unit of 50x1ML |
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Fast EvaGreen Master Mix for qPCR(200 rxn) |
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| 31003 | Biotium | 2x1ML | 244.8 EUR |
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Description: Minimum order quantity: 1 unit of 2x1ML |
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Fast EvaGreen Master Mix for qPCR (500 rxn) |
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| 31003-1 | Biotium | 5x1ML | 463.2 EUR |
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Description: Minimum order quantity: 1 unit of 5x1ML |
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Green-2-Go qPCR Mastermix-ROX (500X20ul Rxn) |
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| QPCR001-R | Bio Basic | 4X1.25ml | 303.25 EUR |
Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (2000 reactions) |
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| 31041-20mL | Biotium | 2x10mL | 592.8 EUR |
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Description: Minimum order quantity: 1 unit of 2x10mL |
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Green-2-Go qPCR Mastermix-iCycler (500X20ul Rxn) |
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| QPCR003-iC | Bio Basic | 4X1.25ml | 303.25 EUR |
RT Master Mix for qPCR (gDNA digester plus) |
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| HY-K0511 | MedChemExpress | 1 mL (100 rxns) | 378 EUR |
Entrans 2X qPCR Probe Master Mix with UDG |
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| RK21222 | Abclonal | 50RXN | 71 EUR |
Green-2-Go qPCR Mastermix-no Dye (500X20ul Rxn) |
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| QPCR006-NODYE | Bio Basic | 4X1.25ml | 326.22 EUR |
Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (500 reactions) |
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| 31041-1 | Biotium | 5x1mL | 326.4 EUR |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (100 reactions) |
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| 31041-T | Biotium | 1mL | 136.8 EUR |
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Description: Minimum order quantity: 1 unit of 1mL |
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Genorise® 2 x qPCR HotStart Master Mix |
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| GR108002 | Genorise Scientific | 2.0 ml | 279 EUR |
Green-2-Go qPCR Mastermix-Low ROX (500X20ul Rxn) |
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| QPCR002-L | Bio Basic | 4X1.25ml | 303.25 EUR |
TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *No ROX* |
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| 17282-1mL | AAT Bioquest | 1 mL | 99 EUR |
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Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays. |
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TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *No ROX* |
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| 17283-5mL | AAT Bioquest | 5 mL | 308 EUR |
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Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays. |
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TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *Low ROX* |
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| 17284-1mL | AAT Bioquest | 1 mL | 99 EUR |
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Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays. |
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TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *Low ROX* |
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| 17285-5mL | AAT Bioquest | 5 mL | 308 EUR |
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Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays. |
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One Step SYBR Green RT-PCR Mix |
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| EQ007 | ELK Biotech | 20 µL x 100 rxn | 248 EUR |
TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *High ROX* |
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| 17286-1mL | AAT Bioquest | 1 mL | 99 EUR |
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Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays. |
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TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *High ROX* |
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| 17287-5mL | AAT Bioquest | 5 mL | 308 EUR |
|
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays. |
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TAQuest™ qPCR Master Mix for TaqMan Probes *No ROX* |
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| 17282 | AAT Bioquest | 1 mL | 99 EUR |
TAQuest™ qPCR Master Mix for TaqMan Probes *No ROX* |
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| 17283 | AAT Bioquest | 5 mL | 308 EUR |
TAQuest™ qPCR Master Mix for TaqMan Probes *Low ROX* |
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| 17284 | AAT Bioquest | 1 mL | 99 EUR |
TAQuest™ qPCR Master Mix for TaqMan Probes *Low ROX* |
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| 17285 | AAT Bioquest | 5 mL | 308 EUR |
Fast Plus EvaGreen qPCR Master Mix (trial size, 100 rxn) |
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| 31020-T | Biotium | 1mL | 160.8 EUR |
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Description: Minimum order quantity: 1 unit of 1mL |
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TAQuest™ qPCR Master Mix for TaqMan Probes *High ROX* |
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| 17286 | AAT Bioquest | 1 mL | 99 EUR |
TAQuest™ qPCR Master Mix for TaqMan Probes *High ROX* |
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| 17287 | AAT Bioquest | 5 mL | 308 EUR |
Green-2-Go TaqProbe qPCR Mastermix-no Dye (500X20ul Rxn) |
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| QPCR005-NODYE | Bio Basic | 4X1.25ml | 303.25 EUR |
TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *No ROX* |
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| 17288-1mL | AAT Bioquest | 1 mL | 99 EUR |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
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TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *No ROX* |
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| 17289-5mL | AAT Bioquest | 5 mL | 308 EUR |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
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iGreen 2X qPCR Master Mix with Low-ROX, 500Rxns-20ul |
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| A4183-4x125ML500Rxns | Biomatik Corporation | 4x1.25ML (500Rxns) | 200.2 EUR |
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Description: Biotechnology |
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TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *Low ROX* |
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| 17290-1mL | AAT Bioquest | 1 mL | 99 EUR |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
|||
TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *Low ROX* |
|||
| 17291-5mL | AAT Bioquest | 5 mL | 308 EUR |
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Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
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TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *High ROX* |
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| 17292-1mL | AAT Bioquest | 1 mL | 99 EUR |
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Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
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TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *High ROX* |
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| 17293-5mL | AAT Bioquest | 5 mL | 308 EUR |
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Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
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Fast Plus EvaGreen qPCR Master Mix with low Rox (500 rxn) |
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| 31014-1 | Biotium | 5x1mL | 524.4 EUR |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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Fast Plus EvaGreen qPCR Master Mix with low Rox (5000 rxn) |
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| 31014-2 | Biotium | 50x1mL | 4291.2 EUR |
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Description: Minimum order quantity: 1 unit of 50x1mL |
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Fast Plus EvaGreen qPCR Master Mix with high Rox (500 rxn) |
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| 31015-1 | Biotium | 5x1mL | 524.4 EUR |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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Fast Plus EvaGreen qPCR Master Mix with high Rox (5000 rxn) |
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| 31015-2 | Biotium | 50x1mL | 4291.2 EUR |
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Description: Minimum order quantity: 1 unit of 50x1mL |
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TAQuest™ FAST qPCR Master Mix for TaqMan Probes *No ROX* |
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| 17288 | AAT Bioquest | 1 mL | 99 EUR |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *No ROX* |
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| 17289 | AAT Bioquest | 5 mL | 308 EUR |
qPCR Master Mix DLP1 (SafeLine: dUTP), packed in 1,25 ml vials |
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| S190 | GeneOn | 100 rcs (2,5 ml) | 165.6 EUR |
qPCR Master Mix DLP1 (SafeLine: dUTP), packed in 1,25 ml vials |
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| S190L | GeneOn | 500 rcs (12,5 ml) | 627.6 EUR |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX* |
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| 17290 | AAT Bioquest | 1 mL | 99 EUR |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX* |
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| 17291 | AAT Bioquest | 5 mL | 308 EUR |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *High ROX* |
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| 17292 | AAT Bioquest | 1 mL | 99 EUR |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *High ROX* |
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| 17293 | AAT Bioquest | 5 mL | 308 EUR |
Fast EvaGreen Master Mix for qPCR, trial size (100 rxn) |
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| 31003-T | Biotium | 1ML | 147.6 EUR |
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Description: Minimum order quantity: 1 unit of 1ML |
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ABScript III RT Master Mix for qPCR with gDNA Remover |
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| RK20429 | Abclonal | 10RXN | 20 EUR |
ABScript Neo RT Master Mix for qPCR with gDNA Remover |
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| RK20433 | Abclonal | 100RXN | 30 EUR |
Fast Plus EvaGreen qPCR Master Mix with low Rox (100 rxn): (1mL) |
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| 31014-T | Biotium | 1mL | 160.8 EUR |
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Description: Minimum order quantity: 1 unit of 1mL |
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Fast Plus EvaGreen qPCR Master Mix with High Rox (100 rxn): (1mL) |
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| 31015-T | Biotium | 1mL | 160.8 EUR |
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Description: Minimum order quantity: 1 unit of 1mL |
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qPCR Master Mix DLP2 (SafeLine: dUTP, UNG), packed in 1,25 ml vials |
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| S200 | GeneOn | 100 rcs (2,5 ml) | 190.8 EUR |
qPCR Master Mix DLP2 (SafeLine: dUTP, UNG), packed in 1,25 ml vials |
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| S200L | GeneOn | 500 rcs (12,5 ml) | 734.4 EUR |
Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (Low ROX) (500 reactions) |
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| 9-31045 | Biotium |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (500 reactions) |
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| 31042-1 | Biotium | 5x1mL | 326.4 EUR |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (200 reactions) |
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| 31042-20mL | Biotium | 2x10mL | 592.8 EUR |
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Description: Minimum order quantity: 1 unit of 2x10mL |
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (100 reactions) |
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| 31042-T | Biotium | 1mL | 136.8 EUR |
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Description: Minimum order quantity: 1 unit of 1mL |
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (High ROX) (500 reactions) |
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| 9-31046 | Biotium |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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Fast Plus EvaGreen Master Mix for qPCR with Rox (200 rxn) |
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| 31014 | Biotium | 2x1mL | 273.6 EUR |
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Description: Minimum order quantity: 1 unit of 2x1mL |
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qPCR Master Mix E1 (SafeLine: EvaGreen, dUTP), packed in 1,25 ml vials |
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| S150 | GeneOn | 100 rcs (2,5 ml) | 165.6 EUR |
qPCR Master Mix E1 (SafeLine: EvaGreen, dUTP), packed in 1,25 ml vials |
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| S150L | GeneOn | 500 rcs (12,5 ml) | 627.6 EUR |
qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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| M-PCR-372L | MiTeGen | 10 x 1,25 ml | 818 EUR |
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Description: qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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| M-PCR-372S | MiTeGen | 2 x 1,25 ml | 205 EUR |
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Description: qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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CRISPY™ Master Mix for qPCR-based Gene Editing Quantitation |
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| CRISPY100A-1 | SBI | 200 rxn | 412 EUR |
qPCR Master Mix E2 (SafeLine: EvaGreen, dUTP, UNG), packed in 1,25 ml vials |
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| S160 | GeneOn | 100 rcs (2,5 ml) | 190.8 EUR |
qPCR Master Mix E2 (SafeLine: EvaGreen, dUTP, UNG), packed in 1,25 ml vials |
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| S160L | GeneOn | 500 rcs (12,5 ml) | 734.4 EUR |
Fast Plus EvaGreen Master Mix for qPCR with High Rox (200 rxn) |
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| 31015 | Biotium | 2x1mL | 273.6 EUR |
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Description: Minimum order quantity: 1 unit of 2x1mL |
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qPCR SybrMaster lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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| M-PCR-373L | MiTeGen | 10 x 1,25 ml | 818 EUR |
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Description: qPCR SybrMaster lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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qPCR SybrMaster lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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| M-PCR-373S | MiTeGen | 2 x 1,25 ml | 205 EUR |
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Description: qPCR SybrMaster lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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qPCR SybrMaster UNGMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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| M-PCR-375L | MiTeGen | 10 x 1,25 ml | 982 EUR |
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Description: qPCR SybrMaster UNGMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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qPCR SybrMaster UNGMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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| M-PCR-375S | MiTeGen | 2 x 1,25 ml | 246 EUR |
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Description: qPCR SybrMaster UNGMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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qPCR Master Mix DLP3 (SafeLine: dUTP, 500 nM ROX), packed in 1,25 ml vials |
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| S210 | GeneOn | 100 rcs (2,5 ml) | 165.6 EUR |
qPCR Master Mix DLP3 (SafeLine: dUTP, 500 nM ROX), packed in 1,25 ml vials |
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| S210L | GeneOn | 500 rcs (12,5 ml) | 627.6 EUR |
2×SYBR-Green-qPCR-Mix |
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| E37F021 | EnoGene | 10×5mL | 1360 EUR |
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Description: DsDNA,-ssDNA,-RNA-quantitative-detection-kit |
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SYBR Green I |
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| B1747-25 | Biovision | each | 1396.8 EUR |
SYBR Green I |
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| B1747-5 | Biovision | each | 464.4 EUR |
qPCR SybrMaster highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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| M-PCR-374L | MiTeGen | 10 x 1,25 ml | 818 EUR |
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Description: qPCR SybrMaster highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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qPCR SybrMaster highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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| M-PCR-374S | MiTeGen | 2 x 1,25 ml | 205 EUR |
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Description: qPCR SybrMaster highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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qPCR Master Mix E5 (EvaGreen, dUTP, 100 nM LOW ROX), packed in 1,25 ml vials |
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| S175 | GeneOn | 100 rcs (2,5 ml) | 165.6 EUR |
qPCR Master Mix E5 (EvaGreen, dUTP, 100 nM LOW ROX), packed in 1,25 ml vials |
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| S175L | GeneOn | 500 rcs (12,5 ml) | 627.6 EUR |
A cell-free, mate-paired library offered single DNA molecules that have been amplified in parallel to 1-micrometer beads by emulsion polymerase chain response. Millions of beads have been immobilized in a polyacrylamide gel and subjected to automated cycles of sequencing by ligation and four-color imaging. Cost per base was roughly one-ninth as a lot as that of standard sequencing. Our protocols have been carried out with off-the-shelf instrumentation and reagents.