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Full-length RNA-seq from single cells using Smart-seq2.

Full-length RNA-seq from single cells using Smart-seq2.

Posted on July 23, 2021 by Zoe

Emerging strategies for the correct quantification of gene expression in particular person cells maintain promise for revealing the extent, operate and origins of cell-to-cell variability. Different high-throughput strategies for single-cell RNA-seq have been launched that fluctuate in protection, sensitivity and multiplexing capability. We lately launched Smart-seq for transcriptome evaluation from single cells, and we subsequently optimized the tactic for improved sensitivity, accuracy and full-length protection throughout transcripts.

Here we current an in depth protocol for Smart-seq2 that enables the era of full-length cDNA and sequencing libraries by using commonplace reagents. The whole protocol takes ∼2 d from cell selecting to having a remaining library prepared for sequencing; sequencing would require an extra 1-Three d relying on the technique and sequencer.

The present limitations are the dearth of strand specificity and the lack to detect nonpolyadenylated (polyA(-)) RNA. Genome modifying pushed by zinc-finger nucleases (ZFNs) yields excessive gene-modification efficiencies (>10%) by introducing a recombinogenic double-strand break into the focused gene. The cleavage occasion is induced using two custom-designed ZFNs that heterodimerize upon binding DNA to type a catalytically lively nuclease advanced. Using the present ZFN structure, nonetheless, cleavage-competent homodimers can also type that may restrict security or efficacy through off-target cleavage.

Here we develop an improved ZFN structure that eliminates this downside. Using structure-based design, we engineer two variant ZFNs that effectively cleave DNA solely when paired as a heterodimer. These ZFNs modify a local endogenous locus as effectively because the parental structure, however with a >40-fold discount in homodimer operate and far decrease ranges of genome-wide cleavage. This structure offers a common means for bettering the specificity of ZFNs as gene modification reagents.

Hyperthermophilic enzymes: sources, makes use of, and molecular mechanisms for thermostability.

Enzymes synthesized by hyperthermophiles (micro organism and archaea with optimum progress temperatures of>> 80 levels C), additionally referred to as hyperthermophilic enzymes, are sometimes thermostable (i.e., immune to irreversible inactivation at excessive temperatures) and are optimally lively at excessive temperatures.

These enzymes share the identical catalytic mechanisms with their mesophilic counterparts. When cloned and expressed in mesophilic hosts, hyperthermophilic enzymes often retain their thermal properties, indicating that these properties are genetically encoded. Sequence alignments, amino acid content material comparisons, crystal construction comparisons, and mutagenesis experiments point out that hyperthermophilic enzymes are, certainly, similar to their mesophilic homologues.

Full-length RNA-seq from single cells using Smart-seq2.

No single mechanism is liable for the exceptional stability of hyperthermophilic enzymes. Increased thermostability should be discovered, as an alternative, in a small variety of extremely particular alterations that usually don’t obey any apparent visitors guidelines. After briefly discussing the range of hyperthermophilic organisms, this evaluate concentrates on the exceptional thermostability of their enzymes.

The biochemical and molecular properties of hyperthermophilic enzymes are described. Mechanisms liable for protein inactivation are reviewed. The molecular mechanisms concerned in protein thermostabilization are mentioned, together with ion pairs, hydrogen bonds, hydrophobic interactions, disulfide bridges, packing, lower of the entropy of unfolding, and intersubunit interactions. Finally, present makes use of and potential functions of thermophilic and hyperthermophilic enzymes as analysis reagents and as catalysts for industrial processes are described.

Nuclear protein import in permeabilized mammalian cells requires soluble cytoplasmic components.

We have developed an in vitro system involving digitonin-permeabilized vertebrate cells to review biochemical occasions within the transport of macromolecules throughout the nuclear envelope. While therapy of cultured cells with digitonin permeabilizes the plasma membranes to macromolecules, the nuclear envelopes stay structurally intact and nuclei retain the power to move and accumulate proteins containing the SV40 giant T antigen nuclear location sequence.

Transport requires addition of exogenous cytosol to permeabilized cells, indicating the soluble cytoplasmic issue(s) required for nuclear import are launched throughout digitonin therapy. In this reconstituted import system, a protein containing a nuclear location sign is quickly amassed in nuclei, the place it reaches a 30-fold focus in comparison with the encircling medium inside 30 min. Nuclear import is particular for a purposeful nuclear location sequence, requires ATP and cytosol, and is temperature dependent.

Furthermore, accumulation of the transport substrate inside nuclei is totally inhibited by wheat germ agglutinin, which binds to nuclear pore complexes and inhibits transport in vivo. Together, these outcomes point out that the permeabilized cell system reproduces genuine nuclear protein import. In a preliminary biochemical dissection of the system, we observe that the sulfhydryl alkylating reagent N-ethylmaleimide inactivates each cytosolic issue(s) and in addition part(s) within the insoluble permeabilized cell fraction required for nuclear protein import.

Because this permeabilized cell mannequin is straightforward, environment friendly, and works successfully with cells and cytosol fractions ready from a wide range of completely different vertebrate sources, it can show highly effective for investigating the biochemical pathway of nuclear transport.

Accurate multiplex polony sequencing of an developed bacterial genome.

We describe a DNA sequencing expertise wherein a generally out there, cheap epifluorescence microscope is transformed to speedy nonelectrophoretic DNA sequencing automation. We apply this expertise to resequence an developed pressure of Escherichia coli at lower than one error per million consensus bases.

AceQ qPCR SYBR® Green Master Mix

Q111-02 Vazyme 500 rxn (20 μl/rxn) 265.2 EUR

AceQ qPCR SYBR® Green Master Mix

Q111-03 Vazyme 2500 rxn (20 μl/rxn) 775.2 EUR

2x SYBR Green qPCR Master Mix (High ROX)

B21402 Bimake 5 ml 268.8 EUR
Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

2x SYBR Green qPCR Master Mix (High ROX)

B21403 Bimake 25 ml 1027.2 EUR
Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

2x SYBR Green qPCR Master Mix (Low ROX)

B21702 Bimake 5 ml 268.8 EUR
Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

2x SYBR Green qPCR Master Mix (Low ROX)

B21703 Bimake 25 ml 1027.2 EUR
Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

AceQ Universal SYBR qPCR Master Mix

Q511-02 Vazyme 500 rxn (20 μl/rxn) 265.2 EUR

AceQ Universal SYBR qPCR Master Mix

Q511-03 Vazyme 2500 rxn (20 μl/rxn) 775.2 EUR

ChamQ Universal SYBR qPCR Master Mix

Q711-02 Vazyme 500 rxn (20 μl/rxn) 265.2 EUR

ChamQ Universal SYBR qPCR Master Mix

Q711-03 Vazyme 2500 rxn (20 μl/rxn) 775.2 EUR

miRNA Universal SYBR® qPCR Master Mix

MQ101-01 Vazyme 125 rxn(20 μl/rxn) 165.6 EUR

miRNA Universal SYBR® qPCR Master Mix

MQ101-02 Vazyme 500 rxn(20 μl/rxn) 290.4 EUR

2X Universal SYBR Green Fast qPCR Mix

RK21203 Abclonal 1 mL Ask for price

Genious 2X SYBR Green Fast qPCR Mix

RK21204 Abclonal 1 mL Ask for price

Genious 2X SYBR GREEN FAST qPCR MIX (No ROX)

RK21205 Abclonal 1 ml Ask for price

RT Master Mix for qPCR

HY-K0510 MedChemExpress 1 mL (100 rxns) 349.2 EUR

AceQ qPCR Probe Master Mix

Q112-02 Vazyme 500 rxn (20 μl/rxn) 265.2 EUR

AceQ qPCR Probe Master Mix

Q112-03 Vazyme 2500 rxn (20 μl/rxn) 775.2 EUR

Entrans 2X qPCR Probe Master Mix

RK21208 Abclonal 40 RXN Ask for price

TAQuest™ qPCR Master Mix with Helixyte™ Green *No ROX*

17270 AAT Bioquest 1 mL 99 EUR

TAQuest™ qPCR Master Mix with Helixyte™ Green *No ROX*

17271 AAT Bioquest 5 mL 308 EUR

TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX*

17272 AAT Bioquest 1 mL 99 EUR

TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX*

17273 AAT Bioquest 5 mL 308 EUR

TAQuest™ qPCR Master Mix with Helixyte™ Green *High ROX*

17274 AAT Bioquest 1 mL 99 EUR

TAQuest™ qPCR Master Mix with Helixyte™ Green *High ROX*

17275 AAT Bioquest 5 mL 308 EUR

amfiSure Ultra Fidelity PCR Master Mix(2X)

P0346-001 GenDepot 1ml 336 EUR

amfiSure Ultra Fidelity PCR Master Mix(2X)

P0346-002 GenDepot 2x1ml 578.4 EUR

amfiSure Ultra Fidelity PCR Master Mix(2X)

P0346-005 GenDepot 5x1ml 993.6 EUR

amfiSure Ultra Fidelity PCR Master Mix(2X)

P0346-010 GenDepot 10x1ml 1632 EUR

lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

M-PCR-173L MiTeGen 960 reactions x 20 µl 789.36 EUR
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

M-PCR-173S MiTeGen 192 reactions x 20 µl 197.34 EUR
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX*

17276 AAT Bioquest 1 mL 99 EUR

TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX*

17277 AAT Bioquest 5 mL 308 EUR

TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX*

17278 AAT Bioquest 1 mL 99 EUR

TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX*

17279 AAT Bioquest 5 mL 308 EUR

TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *High ROX*

17280 AAT Bioquest 1 mL 99 EUR

TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *High ROX*

17281 AAT Bioquest 5 mL 308 EUR

HotStart PCR Master mix (2X, Green Dye)

BS9291 Bio Basic 5X1mL, 5ml 251.4 EUR

HotStart PCR Master mix (2X, Green Dye)

BS9292 Bio Basic 1ml 115.68 EUR

PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500

HS-100-QPCR CORNING 100/pk 381.6 EUR
Description: Sealing Products; Sealing films - Axygen

PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500

HS-150-QPCR CORNING 100/pk 381.6 EUR
Description: Sealing Products; Sealing films - Axygen

SYBR Green I

B1747-25 Biovision 1396.8 EUR

SYBR Green I

B1747-5 Biovision 464.4 EUR

Fast EvaGreen Master Mix for qPCR(200 rxn)

31003 Biotium 2x1ML 244.8 EUR
Description: Minimum order quantity: 1 unit of 2x1ML

Fast EvaGreen Master Mix for qPCR (500 rxn)

31003-1 Biotium 5x1ML 463.2 EUR
Description: Minimum order quantity: 1 unit of 5x1ML

Fast EvaGreen Master Mix for qPCR (5000 rxn)

31003-2 Biotium 50x1ML 3739.2 EUR
Description: Minimum order quantity: 1 unit of 50x1ML

Fast Plus EvaGreen qPCR Master Mix (200 rxn)

31020 Biotium 2x1mL 273.6 EUR
Description: Minimum order quantity: 1 unit of 2x1mL

Fast Plus EvaGreen qPCR Master Mix (500 rxn)

31020-1 Biotium 5x1mL 524.4 EUR
Description: Minimum order quantity: 1 unit of 5x1mL

Fast Plus EvaGreen qPCR Master Mix (5000 rxn)

31020-2 Biotium 50x1mL 4291.2 EUR
Description: Minimum order quantity: 1 unit of 50x1mL

Genorise® 2 x qPCR HotStart Master Mix

GR108002 Genorise Scientific 2.0 ml 334.8 EUR

RT Master Mix for qPCR (gDNA digester plus)

HY-K0511 MedChemExpress 1 mL (100 rxns) 378 EUR

ABScript II One Step SYBR Green RT-qPCR Kit

RK20404 Abclonal 10 RXN Ask for price

Jade? Master Mix

M1105-500 Biovision 529.2 EUR

Taqman Master Mix

M1121-500 Biovision 529.2 EUR

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (500 reactions)

31041-1 Biotium 5x1mL 326.4 EUR
Description: Minimum order quantity: 1 unit of 5x1mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (2000 reactions)

31041-20mL Biotium 2x10mL 592.8 EUR
Description: Minimum order quantity: 1 unit of 2x10mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (100 reactions)

31041-T Biotium 1mL 136.8 EUR
Description: Minimum order quantity: 1 unit of 1mL

EZSolution? SYBR Green I

B1748-1 Biovision 744 EUR

EZSolution? SYBR Green I

B1748-500 Biovision 516 EUR

OPTIONAL GREEN FILTER (572NM)

GDBL-GREEN CORNING 1/pk 1262.4 EUR
Description: Lab Equipment; Axygen Branded EQ

Fast EvaGreen Master Mix for qPCR, trial size (100 rxn)

31003-T Biotium 1ML 147.6 EUR
Description: Minimum order quantity: 1 unit of 1ML

Fast Plus EvaGreen qPCR Master Mix (trial size, 100 rxn)

31020-T Biotium 1mL 160.8 EUR
Description: Minimum order quantity: 1 unit of 1mL

TAQuest™ qPCR Master Mix for TaqMan Probes *No ROX*

17282 AAT Bioquest 1 mL 99 EUR

TAQuest™ qPCR Master Mix for TaqMan Probes *No ROX*

17283 AAT Bioquest 5 mL 308 EUR

TAQuest™ qPCR Master Mix for TaqMan Probes *Low ROX*

17284 AAT Bioquest 1 mL 99 EUR

TAQuest™ qPCR Master Mix for TaqMan Probes *Low ROX*

17285 AAT Bioquest 5 mL 308 EUR

TAQuest™ qPCR Master Mix for TaqMan Probes *High ROX*

17286 AAT Bioquest 1 mL 99 EUR

TAQuest™ qPCR Master Mix for TaqMan Probes *High ROX*

17287 AAT Bioquest 5 mL 308 EUR

Taqman Master Mix-iCycler

M1122-500 Biovision 529.2 EUR

Taqman Master Mix-ROX

M1124-500 Biovision 529.2 EUR

Taqman Master Mix-Multiplex

M1125-500 Biovision 529.2 EUR

amfiSure PCR Master Mix

P0311-010 GenDepot 2x50 rxns 151.2 EUR

amfiSure PCR Master Mix

P0311-025 GenDepot 5X50 rxns 198 EUR

amfiSure PCR Master Mix

P0311-050 GenDepot 10X50 rxns 262.8 EUR

amfiSure PCR Master Mix

P0311-100 GenDepot 10x1ml 343.2 EUR

amfiSure PCR Master Mix

P0311-125 GenDepot 15X50 rxns 333.6 EUR

amfiSure PCR Master Mix

P0311-200 GenDepot 20x1ml 562.8 EUR

amfiSure PCR Master Mix

P0311-250 GenDepot 50x50 rxns 902.4 EUR

amfiSure PCR Master Mix

P0311-500 GenDepot 100x50 rxns 1509.6 EUR

amfiXpand PCR Master Mix

P0331-010 GenDepot 2X50 rxns 181.2 EUR

amfiXpand PCR Master Mix

P0331-025 GenDepot 5X50 rxns 327.6 EUR

amfiXpand PCR Master Mix

P0331-050 GenDepot 10X50 rxns 537.6 EUR

amfiXpand PCR Master Mix

P0331-250 GenDepot 50x50 rxns 2247.6 EUR

2 × Taq Master Mix

P111-01 Vazyme 5 ml 140.4 EUR

2 × Taq Master Mix

P111-02 Vazyme 15 ml 175.2 EUR

2 × Taq Master Mix

P111-03 Vazyme 50 ml 283.2 EUR

2 × AceTaq Master Mix

P411-01 Vazyme 1 ml 142.8 EUR

2 × AceTaq Master Mix

P411-02 Vazyme 5 ml 189.6 EUR

2 × AceTaq Master Mix

P411-03 Vazyme 15 ml 314.4 EUR

Accuris Taq Master Mix

PR1001-1000 Benchmark Scientific 1 PC 360.31 EUR

Accuris Taq Master Mix

PR1001-200 Benchmark Scientific 1 PC 145.94 EUR

Accuris Taq Master Mix

PR1001-S Benchmark Scientific 1 PC 83.75 EUR

Green qPCR SuperMix

20-abx098031 Abbexa
  • 1245.60 EUR
  • 526.80 EUR
  • 760.80 EUR
  • 15 ml
  • 1 ml
  • 5 ml

Green Taq Mix

P131-01 Vazyme 5 ml 142.8 EUR

Green Taq Mix

P131-02 Vazyme 15 ml 180 EUR

Green Taq Mix

P131-03 Vazyme 50 ml 297.6 EUR

CytoTrace™ Ultra Green

21800 AAT Bioquest 1 mg 278 EUR

Fast Plus EvaGreen Master Mix for qPCR with Rox (200 rxn)

31014 Biotium 2x1mL 273.6 EUR
Description: Minimum order quantity: 1 unit of 2x1mL

Fast Plus EvaGreen qPCR Master Mix with low Rox (500 rxn)

31014-1 Biotium 5x1mL 524.4 EUR
Description: Minimum order quantity: 1 unit of 5x1mL

Fast Plus EvaGreen qPCR Master Mix with low Rox (5000 rxn)

31014-2 Biotium 50x1mL 4291.2 EUR
Description: Minimum order quantity: 1 unit of 50x1mL

Fast Plus EvaGreen qPCR Master Mix with high Rox (500 rxn)

31015-1 Biotium 5x1mL 524.4 EUR
Description: Minimum order quantity: 1 unit of 5x1mL

Fast Plus EvaGreen qPCR Master Mix with high Rox (5000 rxn)

31015-2 Biotium 50x1mL 4291.2 EUR
Description: Minimum order quantity: 1 unit of 50x1mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (500 reactions)

31042-1 Biotium 5x1mL 326.4 EUR
Description: Minimum order quantity: 1 unit of 5x1mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (200 reactions)

31042-20mL Biotium 2x10mL 592.8 EUR
Description: Minimum order quantity: 1 unit of 2x10mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (100 reactions)

31042-T Biotium 1mL 136.8 EUR
Description: Minimum order quantity: 1 unit of 1mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (Low ROX) (500 reactions)

9-31045 Biotium
  • 136.80 EUR
  • 578.40 EUR
  • 319.20 EUR
  • 1mL
  • 1X10mL
  • 5x1mL
Description: Minimum order quantity: 1 unit of 5x1mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (High ROX) (500 reactions)

9-31046 Biotium
  • 136.80 EUR
  • 578.40 EUR
  • 319.20 EUR
  • 1mL
  • 1X10mL
  • 5x1mL
Description: Minimum order quantity: 1 unit of 5x1mL

qPCR Master Mix DLP1 (SafeLine: dUTP), packed in 1,25 ml vials

S190 GeneOn 100 rcs (2,5 ml) 165.6 EUR

qPCR Master Mix DLP1 (SafeLine: dUTP), packed in 1,25 ml vials

S190L GeneOn 500 rcs (12,5 ml) 627.6 EUR

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *No ROX*

17288 AAT Bioquest 1 mL 99 EUR

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *No ROX*

17289 AAT Bioquest 5 mL 308 EUR

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX*

17290 AAT Bioquest 1 mL 99 EUR

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX*

17291 AAT Bioquest 5 mL 308 EUR

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *High ROX*

17292 AAT Bioquest 1 mL 99 EUR

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *High ROX*

17293 AAT Bioquest 5 mL 308 EUR

qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

M-PCR-372L MiTeGen 10 x 1,25 ml 720.29 EUR
Description: qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

M-PCR-372S MiTeGen 2 x 1,25 ml 180.14 EUR
Description: qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

Volatile Organics Mix

60-BIG-MIX Scientific Laboratory Supplies 1ML 120.84 EUR

5 Component Mix

AOAC-MIX-1 Scientific Laboratory Supplies EACH 117.42 EUR

qPCR SybrMaster lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

M-PCR-373L MiTeGen 10 x 1,25 ml 720.29 EUR
Description: qPCR SybrMaster lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

qPCR SybrMaster lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

M-PCR-373S MiTeGen 2 x 1,25 ml 180.14 EUR
Description: qPCR SybrMaster lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

qPCR SybrMaster highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

M-PCR-374L MiTeGen 10 x 1,25 ml 720.29 EUR
Description: qPCR SybrMaster highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

qPCR SybrMaster highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

M-PCR-374S MiTeGen 2 x 1,25 ml 180.14 EUR
Description: qPCR SybrMaster highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

qPCR SybrMaster UNGMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

M-PCR-375L MiTeGen 10 x 1,25 ml 864.5 EUR
Description: qPCR SybrMaster UNGMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

qPCR SybrMaster UNGMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

M-PCR-375S MiTeGen 2 x 1,25 ml 216.19 EUR
Description: qPCR SybrMaster UNGMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

Seamless cloning Master Mix (Kit)

B632219 Bio Basic 40RXN, 40prep 319.96 EUR

Taqman Master Mix-Low ROX

M1123-500 Biovision 529.2 EUR

amfiSure Prime PCR Master Mix

P1311-025 GenDepot 5X50 rxns 193.2 EUR

amfiSure Prime PCR Master Mix

P1311-050 GenDepot 10X50 rxns 262.8 EUR

amfiSure Prime PCR Master Mix

P1311-125 GenDepot 15X50 rxns 327.6 EUR

amfiSure Prime PCR Master Mix

P1311-250 GenDepot 50x50 rxns 886.8 EUR

amfiSure Prime PCR Master Mix

P1311-500 GenDepot 100x50 rxns 1485.6 EUR

2 × Taq Plus Master Mix

P211-01 Vazyme 5 ml 160.8 EUR

2 × Taq Plus Master Mix

P211-02 Vazyme 15 ml 231.6 EUR

2 × Taq Plus Master Mix

P211-03 Vazyme 50 ml 466.8 EUR

2 × Rapid Taq Master Mix

P222-01 Vazyme 5 ml (5×1ml) 144 EUR

2 × Rapid Taq Master Mix

P222-02 Vazyme 15 ml (15×1ml) 183.6 EUR

2 × Rapid Taq Master Mix

P222-03 Vazyme 50 ml (50 x 1 ml) 308.4 EUR

2 × Rapid Taq Master Mix

P222-04 Vazyme 50 ml (10×5ml) 308.4 EUR

amfiSure Advanced PCR Master Mix

P2311-025 GenDepot 5X50 rxns 198 EUR

amfiSure Advanced PCR Master Mix

P2311-050 GenDepot 10X50 rxns 267.6 EUR

amfiSure Advanced PCR Master Mix

P2311-125 GenDepot 15X50 rxns 338.4 EUR

amfiSure Advanced PCR Master Mix

P2311-250 GenDepot 50x50 rxns 902.4 EUR

amfiSure Advanced PCR Master Mix

P2311-500 GenDepot 100x50 rxns 1534.8 EUR

2 × Vazyme LAmp Master Mix

P311-01 Vazyme 1 ml 164.4 EUR

2 × Vazyme LAmp Master Mix

P311-02 Vazyme 5 ml 254.4 EUR

2 × Vazyme LAmp Master Mix

P311-03 Vazyme 15 ml 481.2 EUR

2 × Phanta Max Master Mix

P515-01 Vazyme 1 ml 174 EUR

2 × Phanta Max Master Mix

P515-02 Vazyme 5 ml 322.8 EUR

2 × Phanta Max Master Mix

P515-03 Vazyme 15 ml 690 EUR

Accuris Taq Plus Master Mix

PR1001-TP-1000 Benchmark Scientific 1 PC 992.89 EUR

Accuris Taq Plus Master Mix

PR1001-TP-200 Benchmark Scientific 1 PC 291.41 EUR

Accuris Taq Plus Master Mix

PR1001-TP-S Benchmark Scientific 1 PC 83.75 EUR

AceQ U+ Probe Master Mix

Q113-02 Vazyme 500 rxn (20 μl/rxn) 320.4 EUR

AceQ U+ Probe Master Mix

Q113-03 Vazyme 2500 rxn (20 μl/rxn) 1010.4 EUR

amfiRivert cDNA Synthesis Master Mix

R5101-050 GenDepot 50 rxns 498 EUR

amfiRivert cDNA Synthesis Master Mix

R5101-100 GenDepot 2X50 rxns 1016.4 EUR

amfiRivert cDNA Synthesis Master Mix

R5101-200 GenDepot 4X50 rxns 1453.2 EUR

Green qPCR SuperMix UDG

20-abx098032 Abbexa
  • 1362.00 EUR
  • 543.60 EUR
  • 794.40 EUR
  • 15 ml
  • 1 ml
  • 5 ml

Top Green qPCR SuperMix

20-abx098033 Abbexa
  • 994.80 EUR
  • 260.40 EUR
  • 1362.00 EUR
  • 493.20 EUR
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml

Tip Green qPCR SuperMix

20-abx098034 Abbexa
  • 1161.60 EUR
  • 276.00 EUR
  • 1596.00 EUR
  • 560.40 EUR
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml

Fast Plus EvaGreen qPCR Master Mix with low Rox (100 rxn): (1mL)

31014-T Biotium 1mL 160.8 EUR
Description: Minimum order quantity: 1 unit of 1mL

Fast Plus EvaGreen Master Mix for qPCR with High Rox (200 rxn)

31015 Biotium 2x1mL 273.6 EUR
Description: Minimum order quantity: 1 unit of 2x1mL

Fast Plus EvaGreen qPCR Master Mix with High Rox (100 rxn): (1mL)

31015-T Biotium 1mL 160.8 EUR
Description: Minimum order quantity: 1 unit of 1mL

qPCR Master Mix E1 (SafeLine: EvaGreen, dUTP), packed in 1,25 ml vials

S150 GeneOn 100 rcs (2,5 ml) 165.6 EUR

qPCR Master Mix E1 (SafeLine: EvaGreen, dUTP), packed in 1,25 ml vials

S150L GeneOn 500 rcs (12,5 ml) 627.6 EUR
×

A cell-free, mate-paired library offered single DNA molecules that have been amplified in parallel to 1-micrometer beads by emulsion polymerase chain response. Millions of beads have been immobilized in a polyacrylamide gel and subjected to automated cycles of sequencing by ligation and four-color imaging. Cost per base was roughly one-ninth as a lot as that of standard sequencing. Our protocols have been carried out with off-the-shelf instrumentation and reagents.

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  • DMSO cryopreservation is the method of choice to preserve cells for droplet-based single-cell RNA sequencing.
  • DNA
  • Elisa Kits
  • Gerbil
  • Goat
  • Guinea Pig
  • Hamster
  • Helicobacter
  • Horse
  • Human
  • In Freeze-All Strategy, Cumulative Live Birth Rate (CLBR) Is Increasing According to the Number of Blastocysts Formed in Women <40 Undergoing Intracytoplasmic Sperm Injection (ICSI).
  • Influenza
  • Insect
  • Invariant NKT Cells From Donor Lymphocyte Infusions (DLI-iNKTs) Promote ex vivo Lysis of Leukemic Blasts in a CD1d-Dependent Manner.
  • Kangaroo
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  • Manufacturing and preclinical validation of CAR T cells targeting ICAM-1 for advanced thyroid cancer therapy.
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CUSAg IVD Raw Materials Follow

CUSAg acts as an independent brand of CUSABIO (Wuhan Huamei Biotech Co., Ltd), focusing on raw materials for IVD industry.

CUSAg_CUSABIO
cusag_cusabio CUSAg IVD Raw Materials @cusag_cusabio ·
3 Jul 2019

We will attend the 71st Annual Scientific Meeting & Clinical Lab Expo at Anaheim Convention Center. Will you be there, too? We would be pleased to invite you to stop by our booth #2581 and have a friendly chat with us.

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cusag_cusabio CUSAg IVD Raw Materials @cusag_cusabio ·
15 Mar 2019

Dear Friends,

We are going to attend CACLP 2019 in Nanchang next week. We look forward to seeing you at Booth A4-S27!

Best Regards,
CUSAg Team

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cusag_cusabio CUSAg IVD Raw Materials @cusag_cusabio ·
23 Jan 2019

CUSAg will attend CACLP on March 22-24, 2019. We would like to invite you to visit us at Booth A4-S27.

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cusag_cusabio CUSAg IVD Raw Materials @cusag_cusabio ·
23 Jan 2019

We are China TOP 1 Supplier on Lp-PLA2 antibody pairs. Check our validation data through http://www.cusag.org/analysis-cardiovascular-disease-marker-pla2-a-43.html

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cusag_cusabio CUSAg IVD Raw Materials @cusag_cusabio ·
23 Jan 2019

Our 11th anniversary!

4

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  • Antibody
  • DMSO cryopreservation is the method of choice to preserve cells for droplet-based single-cell RNA sequencing.
  • DNA
  • Elisa Kits
  • Gerbil
  • Goat
  • Guinea Pig
  • Hamster
  • Helicobacter
  • Horse
  • Human
  • In Freeze-All Strategy, Cumulative Live Birth Rate (CLBR) Is Increasing According to the Number of Blastocysts Formed in Women <40 Undergoing Intracytoplasmic Sperm Injection (ICSI).
  • Influenza
  • Insect
  • Invariant NKT Cells From Donor Lymphocyte Infusions (DLI-iNKTs) Promote ex vivo Lysis of Leukemic Blasts in a CD1d-Dependent Manner.
  • Kangaroo
  • Killifish
  • Manufacturing and preclinical validation of CAR T cells targeting ICAM-1 for advanced thyroid cancer therapy.
  • PCR
  • PCR Kits
  • Rabbit
  • Rabbit Antibody
  • Rapid Test Kit
  • Rat
  • RNA
  • Compare Appoptosis lab reagents for research
  • Clones env del virus de la inmunodeficiencia humana de tipo 1 procedentes de infecciones agudas y tempranas de subtipo B para evaluaciones normalizadas de anticuerpos neutralizantes provocados por vacunas.
  • Métodos basados en ZFN, TALEN y CRISPR/Cas para la ingeniería genómica.
  • Medición de proteínas mediante ácido bicinchonínico.
  • RNA-seq de longitud completa a partir de células individuales utilizando Smart-seq2.

biology cells unit test biology cells unit test answers culture cells in pure serum culture cells meaning DNA Elisa Kits enzymes for mucus enzymes ingredient enzymes in pcr enzymes in spanish enzymes in the body enzymes liver enzymes medicine enzymes ph level enzymes test enzymes testing enzymes work by equipment rental equipment rentals near me panel de pon panel door panel quilts ideas PCR pcrdfans pcre pcrfy pcrfy stock pcrichard&sons pcrichard.com pcrichard credit card pay bill pcrichard pay bill pcr kits price pcr test pcr testing Proteins recombinant proteins production recombinant proteins sds page gel recombinant proteins technology RNA vector virus example vector virus introduction vector virus relationship victor virus victor virus cartoonmania virus

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