Human immunodeficiency virus type 1 env clones from acute and early subtype B infections for standardized assessments of vaccine-elicited neutralizing antibodies.

Human immunodeficiency virus type 1 env clones from acute and early subtype B infections for standardized assessments of vaccine-elicited neutralizing antibodies.

Posted on by Zoe
Induction of broadly cross-reactive neutralizing antibodies is a excessive precedence for AIDS vaccine growth however one which has confirmed troublesome to be achieved. While most immunogens generate antibodies that neutralize a subset of T-cell-line-adapted strains of human immunodeficiency virus type 1 (HIV-1), none to this point have generated a potent, broadly cross-reactive response in opposition to major isolates of the virus.
Even small increments in immunogen enchancment resulting in will increase in neutralizing antibody titers and cross-neutralizing exercise would speed up vaccine growth; nonetheless, a scarcity of uniformity in goal strains utilized by totally different investigators to evaluate cross-neutralization has made the comparability of vaccine-induced antibody responses troublesome. Thus, there’s an pressing want to determine commonplace panels of HIV-1 reference strains for broad distribution.
To facilitate this, full-length gp160 genes have been cloned from acute and early subtype B infections and characterised for use as reference reagents to evaluate neutralizing antibodies in opposition to clade B HIV-1. Individual gp160 clones have been screened for infectivity as Env-pseudotyped viruses in a luciferase reporter gene assay in JC53-BL (TZM-bl) cells. Functional env clones have been sequenced and their neutralization phenotypes characterised through the use of soluble CD4, monoclonal antibodies, and serum samples from contaminated people and noninfected recipients of a recombinant gp120 vaccine.
Env clones from 12 R5 major HIV-1 isolates have been chosen that weren’t unusually delicate or immune to neutralization and comprised a large spectrum of genetic, antigenic, and geographic range. These reference reagents will facilitate proficiency testing and different validation efforts aimed toward bettering assay efficiency throughout laboratories and can be utilized for standardized assessments of vaccine-elicited neutralizing antibodies.

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