Wnt proteins are lipid-modified and can act as stem cell progress components.
Wnt signalling is concerned in quite a few occasions in animal growth, together with the proliferation of stem cells and the specification of the neural crest. Wnt proteins are probably essential reagents in increasing particular cell varieties, however in distinction to different developmental signalling molecules equivalent to hedgehog proteins and the bone morphogenetic proteins, Wnt proteins have by no means been remoted in an energetic type.
Although Wnt proteins are secreted from cells, secretion is often inefficient and earlier makes an attempt to characterize Wnt proteins have been hampered by their excessive diploma of insolubility. Here we have now remoted energetic Wnt molecules, together with the product of the mouse Wnt3a gene. By mass spectrometry, we discovered the proteins to be palmitoylated on a conserved cysteine.
Enzymatic elimination of the palmitate or site-directed and pure mutations of the modified cysteine end in loss of exercise, and point out that the lipid is essential for signalling. The purified Wnt3a protein induces self-renewal of haematopoietic stem cells, signifying its potential use in tissue engineering.
RGD and different recognition sequences for integrins.
Proteins that include the Arg-Gly-Asp (RGD) attachment web site, along with the integrins that function receptors for them, represent a serious recognition system for cell adhesion. The RGD sequence is the cell attachment web site of a big quantity of adhesive extracellular matrix, blood, and cell floor proteins, and almost half of the over 20 recognized integrins acknowledge this sequence of their adhesion protein ligands.
Some different integrins bind to associated sequences of their ligands. The integrin-binding exercise of adhesion proteins will be reproduced by quick artificial peptides containing the RGD sequence. Such peptides promote cell adhesion when insolubilized onto a floor, and inhibit it when offered to cells in answer. Reagents that bind selectively to just one or just a few of the RGD-directed integrins will be designed by cyclizing peptides with chosen sequences across the RGD and by synthesizing RGD mimics.
As the integrin-mediated cell attachment influences and regulates cell migration, progress, differentiation, and apoptosis, the RGD peptides and mimics can be utilized to probe integrin capabilities in numerous organic programs. Drug design primarily based on the RGD construction could present new therapies for ailments equivalent to thrombosis, osteoporosis, and most cancers.
Quantification of mRNA utilizing real-time reverse transcription PCR (RT-PCR): traits and issues.
The fluorescence-based real-time reverse transcription PCR (RT-PCR) is extensively used for the quantification of steady-state mRNA ranges and is a crucial device for fundamental analysis, molecular medication and biotechnology. Assays are straightforward to carry out, succesful of excessive throughput, and can mix excessive sensitivity with dependable specificity. The know-how is evolving quickly with the introduction of new enzymes, chemistries and instrumentation.
However, whereas real-time RT-PCR addresses many of the difficulties inherent in standard RT-PCR, it has turn out to be more and more clear that it engenders new issues that require pressing consideration. Therefore, along with offering a snapshot of the state-of-the-art in real-time RT-PCR, this overview has a further goal: it’ll describe and talk about critically some of the issues related to deciphering outcomes which can be numerical and lend themselves to statistical evaluation, but whose accuracy is considerably affected by reagent and operator variability.
Reagents that inhibit the ubiquitin-proteasome proteolytic pathway in cells haven’t been out there. Peptide aldehydes that inhibit main peptidase actions of the 20S and 26S proteasomes are proven to cut back the degradation of protein and ubiquitinated protein substrates by 26S particles.
Unlike inhibitors of lysosomal proteolysis, these compounds inhibit the degradation of not solely irregular and short-lived polypeptides but in addition long-lived proteins in intact cells. We used these brokers to check the significance of the proteasome in antigen presentation. When ovalbumin is launched into the cytosol of lymphoblasts, these inhibitors block the presentation on MHC class I molecules of an ovalbumin-derived peptide by stopping its proteolytic technology.
pYLEX1 - Expression Vector |
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FYY203-5MG | Yeastern Biotech | 5mg | Ask for price |
Blunt E1 Expression Kit |
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abx098098-10rxns | Abbexa | 10 rxns | 560.4 EUR |
Blunt E2 Expression Kit |
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abx098873-10rxns | Abbexa | 10 rxns | 560.4 EUR |
RFP expression Adenovirus |
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AVP001 | GenTarget | 1x109 IFU/ml x 200ul | 418.8 EUR |
Description: pre-made RFP expression adenovirus, provided in DMEM medium. |
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CFP expression Adenovirus |
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AVP002 | GenTarget | 1x109 IFU/ml x 200ul | 418.8 EUR |
Description: pre-made CFP expression adenovirus, provided in DMEM medium. |
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GFP expression Adenovirus |
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AVP011 | GenTarget | 1x109 IFU/ml x 200ul | 418.8 EUR |
Description: pre-made GFP expression adenovirus, provided in DMEM medium. |
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YFP expression Adenovirus |
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AVP012 | GenTarget | 1x109 IFU/ml x 200ul | 418.8 EUR |
Description: pre-made YFP expression adenovirus, provided in DMEM medium. |
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BFP expression Adenovirus |
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AVP017 | GenTarget | 1x109 IFU/ml x 200ul | 418.8 EUR |
Description: pre-made BFP expression adenovirus, provided in DMEM medium. |
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h OCT4 expression Adenovirus, in vivo ready |
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AVP013-PBS | GenTarget | 1x1011 IFU/ml x 50ul | 852 EUR |
Description: pre-made adenovirus express human stem cell factor, OCT4, provided in PBS solution for in vivo application. |
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h SOX2 expression Adenovirus, in vivo ready |
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AVP014-PBS | GenTarget | 1x1011 IFU/ml x 50ul | 852 EUR |
Description: pre-made adenovirus express human stem cell factor, SOX2, provided in PBS solution for in vivo application. |
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h LIN28 expression Adenovirus, in vivo ready |
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AVP015-PBS | GenTarget | 1x1011 IFU/ml x 50ul | 852 EUR |
Description: pre-made adenovirus express human stem cell factor, LIN28, provided in PBS solution for in vivo application. |
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Rac1 Expression Vector Set |
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STA-454 | Cell Biolabs | 1 kit | 1765.2 EUR |
Description: Rac1 Expression Vector Set contains 3 vectors: Rac wild type, T17N dominant negative mutant, and G12V constitutively active mutant. |
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RhoA Expression Vector Set |
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STA-456 | Cell Biolabs | 1 kit | 1765.2 EUR |
Description: RhoA Expression Vector Set contains 3 vectors: RhoA wild type, T19N dominant negative mutant, and G14V constitutively active mutant. |
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Cdc42 Expression Vector Set |
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STA-455 | Cell Biolabs | 1 kit | 1765.2 EUR |
Description: Cdc42 Expression Vector Set contains 3 vectors: Cdc42 wild type, T17N dominant negative mutant, and Q61L constitutively active mutant. |
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h ADA Expression Lentivirus |
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LVP923 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: ADA, containing a RFP-Blasticidin dual selection marker. |
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h IL6 Expression Lentivirus |
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LVP1144 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker. |
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h CD4 Expression Lentivirus |
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LVP1152 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: CD4, containing a RFP-Blasticidin dual selection marker. |
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h IL4 Expression Lentivirus |
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LVP1161 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: IL4, containing a RFP-Blasticidin dual selection marker. |
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h IL7 Expression Lentivirus |
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LVP1162 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: IL7, containing a RFP-Blasticidin dual selection marker. |
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h KDR Expression Lentivirus |
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LVP1176 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: KDR, containing a RFP-Blasticidin dual selection marker. |
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h FAS Expression Lentivirus |
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LVP1181 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: FAS, containing a RFP-Blasticidin dual selection marker. |
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pAAV-MCS Expression Vector |
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VPK-410 | Cell Biolabs | 10 µg | 776.4 EUR |
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV. |
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h OCT4 expression Adenovirus |
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AVP013 | GenTarget | 1x109 IFU/ml x 200ul | 418.8 EUR |
Description: pre-made adenovirus express human stem cell factor, OCT4, provided in DMEM medium. |
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h SOX2 expression Adenovirus |
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AVP014 | GenTarget | 1x109 IFU/ml x 50ul | 418.8 EUR |
Description: pre-made adenovirus express human stem cell factor, SOX2, provided in DMEM medium. |
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h CDR1 Expression Lentivirus |
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LVP917 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: CDR1, containing a RFP-Blasticidin dual selection marker. |
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h CD47 Expression Lentivirus |
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LVP918 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: CD47, containing a RFP-Blasticidin dual selection marker. |
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h ESR1 Expression Lentivirus |
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LVP924 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: ESR1, containing a RFP-Blasticidin dual selection marker. |
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r Syn1 Expression Lentivirus |
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LVP925 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for rat target: Syn1, containing a RFP-Blasticidin dual selection marker. |
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h NME4 Expression Lentivirus |
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LVP932 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: NME4, containing a RFP-Blasticidin dual selection marker. |
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h EGFR Expression Lentivirus |
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LVP1142 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: EGFR, containing a RFP-Blasticidin dual selection marker. |
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h NRP1 Expression Lentivirus |
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LVP1145 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker. |
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h MUC1 Expression Lentivirus |
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LVP1148 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker. |
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h CD8a Expression Lentivirus |
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LVP1151 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: CD8a, containing a RFP-Blasticidin dual selection marker. |
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h PIGF Expression Lentivirus |
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LVP1154 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: PIGF, containing a RFP-Blasticidin dual selection marker. |
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h BRAF Expression Lentivirus |
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LVP1156 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: BRAF, containing a RFP-Blasticidin dual selection marker. |
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h ROS1 Expression Lentivirus |
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LVP1158 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: ROS1, containing a RFP-Blasticidin dual selection marker. |
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h CD3E Expression Lentivirus |
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LVP1163 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: IL7, containing a RFP-Blasticidin dual selection marker. |
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h KLK3 Expression Lentivirus |
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LVP1171 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: KLK3, containing a RFP-Blasticidin dual selection marker. |
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h NEK6 Expression Lentivirus |
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LVP1182 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: NEK6, containing a RFP-Blasticidin dual selection marker. |
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h NEK7 Expression Lentivirus |
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LVP1183 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: NEK7, containing a RFP-Blasticidin dual selection marker. |
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h LIN28 expression Adenovirus |
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AVP015 | GenTarget | 1x109 IFU/ml x 200ul | 418.8 EUR |
Description: pre-made adenovirus express human stem cell factor, LIN28, provided in DMEM medium. |
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h GPNMB Expression Lentivirus |
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LVP921 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: GPNMB, containing a RFP-Blasticidin dual selection marker. |
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h DECR1 Expression Lentivirus |
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LVP927 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: DECR1, containing a RFP-Blasticidin dual selection marker. |
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h DECR2 Expression Lentivirus |
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LVP928 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: DECR2, containing a RFP-Blasticidin dual selection marker. |
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h NMRK1 Expression Lentivirus |
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LVP930 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: NMRK1, containing a RFP-Blasticidin dual selection marker. |
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h PITX3 Expression Lentivirus |
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LVP931 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: PITX3, containing a RFP-Blasticidin dual selection marker. |
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h HDAC1 Expression Lentivirus |
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LVP1143 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker. |
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h VEGFB Expression Lentivirus |
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LVP1146 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker. |
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h PARP1 Expression Lentivirus |
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LVP1149 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker. |
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h IL3RA Expression Lentivirus |
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LVP1150 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: IL3RA, containing a RFP-Blasticidin dual selection marker. |
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h PTCH1 Expression Lentivirus |
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LVP1155 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: PTCH1, containing a RFP-Blasticidin dual selection marker. |
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h SART3 Expression Lentivirus |
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LVP1159 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: SART3, containing a RFP-Blasticidin dual selection marker. |
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h TIGIT Expression Lentivirus |
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LVP1160 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: TIGIT, containing a RFP-Blasticidin dual selection marker. |
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h EDNRA Expression Lentivirus |
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LVP1165 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: EDNRA containing a RFP-Blasticidin dual selection marker. |
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h Foxp3 Expression Lentivirus |
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LVP1172 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: Foxp3, containing a RFP-Blasticidin dual selection marker. |
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m RIKEN Expression Lentivirus |
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LVP1174 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for mouse target: RIKEN, containing a RFP-Blasticidin dual selection marker. |
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h APLP2 Expression Lentivirus |
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LVP1177 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: APLP2, containing a RFP-Blasticidin dual selection marker. |
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h PCSK9 Expression Lentivirus |
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LVP1178 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: PCSK9, containing a RFP-Blasticidin dual selection marker. |
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h NTSR1 Expression Lentivirus |
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LVP1179 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: NTSR1, containing a RFP-Blasticidin dual selection marker. |
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pscAAV-MCS Expression Vector |
|||
VPK-430 | Cell Biolabs | 10 µg | 776.4 EUR |
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV. |
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H-Ras Expression Vector Set |
|||
STA-457 | Cell Biolabs | 1 kit | 1765.2 EUR |
Description: H-Ras Expression Vector Set contains 3 vectors: H-Ras wild type, T19N dominant negative mutant, and G12V constitutively active mutant. |
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m CTAGA1 Expression Lentivirus |
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LVP916 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for mouse target: CTAGA1, containing a RFP-Blasticidin dual selection marker. |
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h SKIV2L Expression Lentivirus |
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LVP919 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: SKIV2L, containing a RFP-Blasticidin dual selection marker. |
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h SKIV2L Expression Lentivirus |
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LVP919-GP | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: SKIV2L, containing a GFP-Puromycin dual selection marker. |
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h TP5313 Expression Lentivirus |
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LVP926 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: TP5313, containing a RFP-Blasticidin dual selection marker. |
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h ELOVL5 Expression Lentivirus |
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LVP929 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: ELOVL5, containing a RFP-Blasticidin dual selection marker. |
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h MAGEA3 Expression Lentivirus |
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LVP1147 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker. |
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h NANOS3 Expression Lentivirus |
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LVP1164 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: NANOS3, containing a RFP-Blasticidin dual selection marker. |
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h IL1RL1 Expression Lentivirus |
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LVP1168 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: IL1RL1, containing a RFP-Blasticidin dual selection marker. |
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h CTAG1B Expression Lentivirus |
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LVP1169 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: CTAG1B, containing a RFP-Blasticidin dual selection marker. |
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h MAGEA3 Expression Lentivirus |
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LVP1170 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: MAGEA3, containing a RFP-Blasticidin dual selection marker. |
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h COX7A1 Expression Lentivirus |
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LVP1173 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: COX7A1, containing a RFP-Blasticidin dual selection marker. |
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h BCL11B Expression Lentivirus |
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LVP1180 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: BCL11B, containing a RFP-Blasticidin dual selection marker. |
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pUC57-sgRNA expression vector |
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PVT18003 | Lifescience Market | 2 ug | 309.6 EUR |
BRAF, His-Tag (High Expression) |
|||
41237 | BPS Bioscience | 10 µg | 375 EUR |
Description: Human BRAF, also known as B-RAF1 and RAFB1, with 18 mutations to improve expression; S446A, S447A, I543A, I544S, I551K, Q562R, L588N, K630S, F667E, Y672S, A688R, L706S, Q709R, S713E, L716E, S720E, P722S, K723G. GenBank Accession #NM_004333, a.a. 444-723 with N-terminal His-tag, expressed in E. coli cells. MW = 33 kDa. |
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ANPRA/Aequorin Expression vector |
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60641 | BPS Bioscience | 20 µg | 900 EUR |
Description: The ANPRA/Aequorin expression vector is designed to co-express human atrial natriuretic peptide receptor A (ANPRA, also called natriuretic peptide receptor A/guanylate cyclase A) and jellyfish (Aequorea victoria) Aequorin in mammalian cells. |
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h CEACAM5 Expression Lentivirus |
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LVP1153 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: CEACAM5, containing a RFP-Blasticidin dual selection marker. |
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h TNFSF11 Expression Lentivirus |
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LVP1157 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: TNFSF11, containing a RFP-Blasticidin dual selection marker. |
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h TNFRSF1B Expression Lentivirus |
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LVP922 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: TNFRSF1B, containing a RFP-Blasticidin dual selection marker. |
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h KIAA1324 Expression Lentivirus |
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LVP1175 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: KIAA1324, containing a RFP-Blasticidin dual selection marker. |
|||
GFP-Rac1 Expression Vector Set |
|||
STA-450 | Cell Biolabs | 1 kit | 1939.2 EUR |
Description: GFP-Rac1 Expression Vector Set contains 3 vectors: Rac wild type, T17N dominant negative mutant, and Q61L constitutively active mutant. Each vector also contains a GFP reporter sequence. |
|||
GFP-RhoA Expression Vector Set |
|||
STA-452 | Cell Biolabs | 1 kit | 1939.2 EUR |
Description: GFP-RhoA Expression Vector Set contains 3 vectors: RhoA wild type, T19N dominant negative mutant, and Q63L constitutively active mutant. Each vector also contains a GFP reporter sequence. |
|||
h KIAA0319L Expression Lentivirus |
|||
LVP920 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for human target: KIAA0319L, containing a RFP-Blasticidin dual selection marker. |
|||
GFP-Cdc42 Expression Vector Set |
|||
STA-451 | Cell Biolabs | 1 kit | 1939.2 EUR |
Description: GFP-Cdc42 Expression Vector Set contains 3 vectors: Cdc42 wild type, T17N dominant negative mutant, and Q61L constitutively active mutant. Each vector also contains a GFP reporter sequence. |
|||
Active Rac1 Expression Vector Set |
|||
STA-458 | Cell Biolabs | 1 kit | 1765.2 EUR |
Description: Active Rac1 Expression Vector Set contains 3 vectors expressing different constitutively active mutants of Rac1: Q61L, Q61L/F37A, and Q61L/Y40C. |
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PhiC31 Integrase Expression Plasmid |
|||
FC200PA-1 | SBI | 10ug | 496 EUR |
pCMV-GFP-LC3 Expression Vector |
|||
CBA-401 | Cell Biolabs | 100 µL | 1243.2 EUR |
Description: Expression vector contains a fusion of GFP and LC3. A separate GFP control vector is also included. |
|||
h KRAS (G12D) Expression Lentivirus |
|||
LVP1166 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for a mutant (G12D) of human target: KRAS containing a RFP-Blasticidin dual selection marker. |
|||
Tet-on circRNA Expression Vector |
|||
PVT14643 | Lifescience Market | 2 ug | 843.6 EUR |
h TP53 (R175H) Expression Lentivirus |
|||
LVP1167 | GenTarget | 1x107 IFU/ml x 200ul | 541.2 EUR |
Description: Pre-made over-expression lentivirus for a mutant R175H) of human target: TP53, containing a RFP-Blasticidin dual selection marker. |
|||
pAAV-IRES-Neo Expression Vector |
|||
VPK-416 | Cell Biolabs | 10 µg | 776.4 EUR |
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV. |
|||
pAAV-IRES-GFP Expression Vector |
|||
VPK-418 | Cell Biolabs | 10 µg | 776.4 EUR |
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV. |
|||
pAAV-IRES-Bsd Expression Vector |
|||
VPK-419 | Cell Biolabs | 10 µg | 776.4 EUR |
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV. |
|||
PinPoint Integrase Expression Plasmid |
|||
PIN200A-1 | SBI | 10ug | 688 EUR |
pAAV-IRES-Puro Expression Vector |
|||
VPK-415 | Cell Biolabs | 10 µg | 776.4 EUR |
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV. |
|||
CEACAM5 CHO Cell Line (Low Expression) |
|||
78704-L | BPS Bioscience | 2 vials | 7150 EUR |
Description: Recombinant clonal CHO cell line stably expressing full-length human CEACAM5 (NM_004363). Surface expression of CEACAM5 was confirmed by flow cytometry. The stable clonal cell line was selected for different levels of CEACAM5 expression (High, Medium, and Low) compared to the parental CHO-K1 cell line. |
|||
Active H-Ras Expression Vector Set |
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STA-459 | Cell Biolabs | 1 kit | 1765.2 EUR |
Description: Active H-Ras Expression Vector Set contains 3 vectors expressing different constitutively active mutants of H-Ras: V12, V12S35, and V12C40. |
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B7-H3 CHO Cell Line (Low Expression) |
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78541-L | BPS Bioscience | 2 vials | 7150 EUR |
Description: B7 homolog 3 protein (B7-H3) CHO Cell line is a recombinant clonal stable CHO cell line expressing full length human B7-H3 protein, also known as cluster of differentiation 276 (CD276, B7H3; B7RP-2; 4Ig-B7-H3). Surface expression of B7-H3 was confirmed by flow cytometry. Each stable clonal cell line was selected for High, Medium, or Low levels of B7-H3 expression to mimic different B7-H3 expression levels in various cancer types. |
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pAAV-IRES-Hygro Expression Vector |
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VPK-417 | Cell Biolabs | 10 µg | 776.4 EUR |
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV. |
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CD36 HEK293 Cell Line (High Expression) |
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78526-H | BPS Bioscience | 2 vials | 7150 EUR |
Description: Recombinant clonal HEK293 cell line stably expressing full length human CD36. The stable clonal cell line was selected for medium and high levels of CD36 expression compared to the parental HEK293 cell line. |
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CEACAM5 CHO Cell Line (High Expression) |
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78704-H | BPS Bioscience | 2 vials | 7150 EUR |
Description: Recombinant clonal CHO cell line stably expressing full-length human CEACAM5 (NM_004363). Surface expression of CEACAM5 was confirmed by flow cytometry. The stable clonal cell line was selected for different levels of CEACAM5 expression (High, Medium, and Low) compared to the parental CHO-K1 cell line. |
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AAV-1 Helper Free Expression System |
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VPK-410-SER1 | Cell Biolabs | 1 kit | 1486.8 EUR |
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). |
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AAV-2 Helper Free Expression System |
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VPK-410-SER2 | Cell Biolabs | 1 kit | 1486.8 EUR |
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). |
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AAV-3 Helper Free Expression System |
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VPK-410-SER3 | Cell Biolabs | 1 kit | 1486.8 EUR |
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). |
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AAV-4 Helper Free Expression System |
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VPK-410-SER4 | Cell Biolabs | 1 kit | 1486.8 EUR |
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). |
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AAV-5 Helper Free Expression System |
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VPK-410-SER5 | Cell Biolabs | 1 kit | 1486.8 EUR |
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). |
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AAV-6 Helper Free Expression System |
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VPK-410-SER6 | Cell Biolabs | 1 kit | 1486.8 EUR |
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). |
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B7-H3 CHO Cell Line (High Expression) |
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78541-H | BPS Bioscience | 2 vials | 7150 EUR |
Description: B7 homolog 3 protein (B7-H3) CHO Cell line is a recombinant clonal stable CHO cell line expressing full length human B7-H3 protein, also known as cluster of differentiation 276 (CD276, B7H3; B7RP-2; 4Ig-B7-H3). Surface expression of B7-H3 was confirmed by flow cytometry. Each stable clonal cell line was selected for High, Medium, or Low levels of B7-H3 expression to mimic different B7-H3 expression levels in various cancer types. |
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AAV-DJ Helper Free Expression System |
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VPK-410-DJ | Cell Biolabs | 1 kit | 1486.8 EUR |
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2. |
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Gryphon Retroviral Expression System I |
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ABP-RVK-10001 | Allele Biotech | 1 set | Ask for price |
Description: PRODUCT LINE DISCONTINUED. Please contact us. |
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Gryphon Retroviral Expression System II |
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ABP-RVK-10002 | Allele Biotech | 1 set | Ask for price |
Description: PRODUCT LINE DISCONTINUED. Please contact us. |
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CD7 CHO Cell Line (Medium or High Expression)-78324-H |
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78324-H | BPS Bioscience | 2 vials | 7150 EUR |
Description: Recombinant CD7 CHO-K1 cell lines expressing full-length human CD7 receptor (accession number: NM_006137.6). Surface expression of human CD7 was confirmed by flow cytometry. Each stable clonal cell line was selected for high or medium levels of CD7 expression to mimic different stages of cancer target cells with various CD7 expression levels. |
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CD7 CHO Cell Line (Medium or High Expression)-78324-M |
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78324-M | BPS Bioscience | 2 vials | 7150 EUR |
Description: Recombinant CD7 CHO-K1 cell lines expressing full-length human CD7 receptor (accession number: NM_006137.6). Surface expression of human CD7 was confirmed by flow cytometry. Each stable clonal cell line was selected for high or medium levels of CD7 expression to mimic different stages of cancer target cells with various CD7 expression levels. |
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CD36 HEK293 Cell Line (Medium Expression) |
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78526-M | BPS Bioscience | 2 vials | 7150 EUR |
Description: Recombinant clonal HEK293 cell line stably expressing full length human CD36. The stable clonal cell line was selected for medium and high levels of CD36 expression compared to the parental HEK293 cell line. |
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CEACAM5 CHO Cell Line (Medium Expression) |
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78704-M | BPS Bioscience | 2 vials | 7150 EUR |
Description: Recombinant clonal CHO cell line stably expressing full-length human CEACAM5 (NM_004363). Surface expression of CEACAM5 was confirmed by flow cytometry. The stable clonal cell line was selected for different levels of CEACAM5 expression (High, Medium, and Low) compared to the parental CHO-K1 cell line. |
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Human Myelin expression factor 2 (MYEF2) |
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1-CSB-EP015283HU | Cusabio |
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Description: Recombinant Human Myelin expression factor 2(MYEF2),partial expressed in E.coli |
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scAAV-1 Helper Free Expression System |
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VPK-430-SER1 | Cell Biolabs | 1 kit | 1486.8 EUR |
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). |
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scAAV-2 Helper Free Expression System |
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VPK-430-SER2 | Cell Biolabs | 1 kit | 1486.8 EUR |
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). |
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scAAV-3 Helper Free Expression System |
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VPK-430-SER3 | Cell Biolabs | 1 kit | 1486.8 EUR |
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). |
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scAAV-4 Helper Free Expression System |
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VPK-430-SER4 | Cell Biolabs | 1 kit | 1486.8 EUR |
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). |
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scAAV-5 Helper Free Expression System |
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VPK-430-SER5 | Cell Biolabs | 1 kit | 1486.8 EUR |
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). |
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scAAV-6 Helper Free Expression System |
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VPK-430-SER6 | Cell Biolabs | 1 kit | 1486.8 EUR |
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). |
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B7-H3 CHO Cell Line (Medium Expression) |
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78541-M | BPS Bioscience | 2 vials | 7150 EUR |
Description: B7 homolog 3 protein (B7-H3) CHO Cell line is a recombinant clonal stable CHO cell line expressing full length human B7-H3 protein, also known as cluster of differentiation 276 (CD276, B7H3; B7RP-2; 4Ig-B7-H3). Surface expression of B7-H3 was confirmed by flow cytometry. Each stable clonal cell line was selected for High, Medium, or Low levels of B7-H3 expression to mimic different B7-H3 expression levels in various cancer types. |
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CD95 CHO Cell Line (Medium or High Expression)-78499-H |
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78499-H | BPS Bioscience | 2 vials | 7150 EUR |
Description: The CD95 CHO cell line is a recombinant clonal stable CHO cell line constitutively expressing full-length human CD95 receptor (accession number: NM_000043.3). Surface expression of human CD95 was confirmed by flow cytometry. Each clonal cell line was selected for different levels of CD95 expression (High or Medium) to mimic varying CD95 expression levels in cancer cells. |
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CD95 CHO Cell Line (Medium or High Expression)-78499-M |
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78499-M | BPS Bioscience | 2 vials | 7150 EUR |
Description: The CD95 CHO cell line is a recombinant clonal stable CHO cell line constitutively expressing full-length human CD95 receptor (accession number: NM_000043.3). Surface expression of human CD95 was confirmed by flow cytometry. Each clonal cell line was selected for different levels of CD95 expression (High or Medium) to mimic varying CD95 expression levels in cancer cells. |
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Protein Limb Expression 1 (LIX1) Antibody |
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20-abx318393 | Abbexa |
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Protein Limb Expression 1 (LIX1) Antibody |
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abx146234-100ug | Abbexa | 100 ug | 469.2 EUR |
Protein Limb Expression 1 (LIX1) Antibody |
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abx025913-400ul | Abbexa | 400 ul | 627.6 EUR |
Protein Limb Expression 1 (LIX1) Antibody |
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abx025913-80l | Abbexa | 80 µl | 343.2 EUR |
scAAV-DJ Helper Free Expression System |
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VPK-430-DJ | Cell Biolabs | 1 kit | 1486.8 EUR |
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2. |
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pRedPuro shRNA expression lentivector (EF1) |
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SI516A-1 | SBI | 10 ug | 594 EUR |
pAAV-MCS Promoterless Expression Vector |
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VPK-411 | Cell Biolabs | 10 µg | 776.4 EUR |
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV. |
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pSMPUW-Neo Lentiviral Expression Vector |
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VPK-213 | Cell Biolabs | 10 µg | 748.8 EUR |
Description: Clone your gene of interest into this Lentiviral Expression Vector, then co-transfect along with lentiviral packaging vectors into a packaging cell line such as 293LTV. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to its design it is best matched with our ViraSafe packaging vectors to produce the highest viral titer. |
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Claudin-6 CHO Cell Line (Low Expression) |
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78527-L | BPS Bioscience | 2 vials | 7150 EUR |
Description: Recombinant clonal CHO cell line stably expressing full-length human claudin-6 (CLDN6 gene accession number NM_021195.5). Surface expression of claudin 6 was confirmed by flow cytometry. The stable clonal cell line was selected for high, medium, or low levels of claudin-6 expression compared to the parental CHO-K1 cell line. |
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RAPAd® RSV Adenoviral Expression System |
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VPK-251 | Cell Biolabs | 1 kit | 1243.2 EUR |
Description: Making an adenovirus with traditional recombinant methods takes 2-3 months and requires tedious plaque recombination. More recent technologies have shortened this time somewhat, but still produce relatively high amounts of wild type (replication-competent) plaques, levels of which increase with serial amplification. The RAPAd® Adenoviral Expression Systems produce recombinant adenovirus with substantially reduced wild-type virus, while considerably shortening the production time to 2-3 weeks. Serial amplification of adenovirus produced using the RAPAd® system does not significantly increase replication-competent adenovirus levels. The system uses a backbone vector from which the 5' ITR, packaging signal and E1 sequences have been removed. |
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RAPAd® CMV Adenoviral Expression System |
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VPK-252 | Cell Biolabs | 1 kit | 1243.2 EUR |
Description: Making an adenovirus with traditional recombinant methods takes 2-3 months and requires tedious plaque recombination. More recent technologies have shortened this time somewhat, but still produce relatively high amounts of wild type (replication-competent) plaques, levels of which increase with serial amplification. The RAPAd® Adenoviral Expression Systems produce recombinant adenovirus with substantially reduced wild-type virus, while considerably shortening the production time to 2-3 weeks. Serial amplification of adenovirus produced using the RAPAd® system does not significantly increase replication-competent adenovirus levels. The system uses a backbone vector from which the 5' ITR, packaging signal and E1 sequences have been removed. |
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pSMPUW-Puro Lentiviral Expression Vector |
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VPK-212 | Cell Biolabs | 10 µg | 748.8 EUR |
Description: Clone your gene of interest into this Lentiviral Expression Vector, then co-transfect along with lentiviral packaging vectors into a packaging cell line such as 293LTV. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to its design it is best matched with our ViraSafe packaging vectors to produce the highest viral titer. |
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Claudin-6 CHO Cell Line (High Expression) |
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78527-H | BPS Bioscience | 2 vials | 7150 EUR |
Description: Recombinant clonal CHO cell line stably expressing full-length human claudin-6 (CLDN6 gene accession number NM_021195.5). Surface expression of claudin 6 was confirmed by flow cytometry. The stable clonal cell line was selected for high, medium, or low levels of claudin-6 expression compared to the parental CHO-K1 cell line. |
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AAV-DJ/8 Helper Free Expression System |
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VPK-410-DJ-8 | Cell Biolabs | 1 kit | 1486.8 EUR |
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2. AAV-DJ/8 was created by making a point mutation in the heparin binding domain of AAV-DJ. Recombinant AAV produced from the AAV-DJ/8 system closely resembles AAV-8 and AAV-9 in its ability to infect heart and brain tissues. |
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Myelin Expression Factor 2 (MYEF2) Antibody |
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abx235464-100ug | Abbexa | 100 ug | 577.2 EUR |
Myelin Expression Factor 2 (MYEF2) Antibody |
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20-abx109333 | Abbexa |
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Myelin Expression Factor 2 (MYEF2) Antibody |
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20-abx113906 | Abbexa |
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Expression Negative Control Lentivirus (G418) |
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79902-G | BPS Bioscience | 500 µl x 2 | 795 EUR |
Description: The Expression Negative Control Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The controls package the same virus particles as the target expression virus, but they do not express a specific protein under the CMV promoter. _x000D_The Expression Negative Control Lentivirus (G418) expresses the gene for aminoglycoside 3' phosphotransferase, which confers resistance to kanamycin, neomycin, and geneticin (G418)._x000D_The Expression Negative Control Lentivirus (Hygromycin) expresses the gene for hygromycin B phosphotransferase, which confers resistance to Hygromycin._x000D_The Expression Negative Control Lentivirus (Puromycin) expresses the gene for puromycin N-acetyl-transferase, which confers resistance to puromycin._x000D_ _x000D_ |
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pGreenPuro shRNA expression lentivector (CMV) |
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SI505A-1 | SBI | 10 ug | 594 EUR |
pGreenPuro shRNA expression lentivector (EF1) |
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SI506A-1 | SBI | 10 ug | 594 EUR |
pSMPUW-Hygro Lentiviral Expression Vector |
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VPK-214 | Cell Biolabs | 10 µg | 748.8 EUR |
Description: Clone your gene of interest into this Lentiviral Expression Vector, then co-transfect along with lentiviral packaging vectors into a packaging cell line such as 293LTV. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to its design it is best matched with our ViraSafe packaging vectors to produce the highest viral titer. |
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Baculovirus expression vector pAc-g-CH3 |
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30R-AB001 | Fitzgerald | 5 ug | 739.2 EUR |
Description: Baculovirus cassette vector pAc-l-CH3 for the expression of human, humanized or chimeric IgG(lambda) in insect cells and secretion of assembled antibodies into the supernatant. |
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Genorise® Chicken CCL7 Expression Plasmid |
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GR148007 | Genorise Scientific | 10 µg | 219 EUR |
RAPAd® miRNA Adenoviral Expression System |
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VPK-253 | Cell Biolabs | 1 kit | 1243.2 EUR |
Description: Making an adenovirus with traditional recombinant methods takes 2-3 months and requires tedious plaque recombination. More recent technologies have shortened this time somewhat, but still produce relatively high amounts of wild type (replication-competent) plaques, levels of which increase with serial amplification. The RAPAd® Adenoviral Expression Systems produce recombinant adenovirus with substantially reduced wild-type virus, while considerably shortening the production time to 2-3 weeks. Serial amplification of adenovirus produced using the RAPAd® system does not significantly increase replication-competent adenovirus levels. The system uses a backbone vector from which the 5' ITR, packaging signal and E1 sequences have been removed. |
|||
Claudin-6 CHO Cell Line (Medium Expression) |
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78527-M | BPS Bioscience | 2 vials | 7150 EUR |
Description: Recombinant clonal CHO cell line stably expressing full-length human claudin-6 (CLDN6 gene accession number NM_021195.5). Surface expression of claudin 6 was confirmed by flow cytometry. The stable clonal cell line was selected for high, medium, or low levels of claudin-6 expression compared to the parental CHO-K1 cell line. |
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scAAV-DJ/8 Helper Free Expression System |
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VPK-430-DJ-8 | Cell Biolabs | 1 kit | 1486.8 EUR |
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2. AAV-DJ/8 was created by making a point mutation in the heparin binding domain of AAV-DJ. Recombinant AAV produced from the AAV-DJ/8 system closely resembles AAV-8 and AAV-9 in its ability to infect heart and brain tissues. |
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pC001 - huLshC2C2- MBP for bacterial expression |
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PVT10645 | Lifescience Market | 2 ug | 361.2 EUR |
Exoenzyme C3 (Rho Inhibitor) Expression Vector |
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STA-460 | Cell Biolabs | 1 kit | 860.4 EUR |
Description: This mammalian expression vector codes for Exoenzyme C3, a Rho inhibitor. |
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Protein Limb Expression 1 (LIX1) Antibody (HRP) |
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20-abx308975 | Abbexa |
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LEXSYcon2.1 Expression Kit M-EGE-1310ble ** shipped on blue ice/Dry Ice (two separate boxes) - must be shipped overnight |
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M-EGE-1310ble | MiTeGen | 1 Kit | 1682 EUR |
Description: LEXSYcon2.1 Expression Kit M-EGE-1310ble ** shipped on blue ice/Dry Ice (two separate boxes) - must be shipped overnight |
By stopping peptide manufacturing from cell proteins, these inhibitors block the meeting of class I molecules. Therefore, the proteasome catalyzes the degradation of the overwhelming majority of cell proteins and generates most peptides offered on MHC class I molecules.