PCR Kits

Commercial PCR kits are available, ready-to-use, and optimized for specific conditions, helping to eliminate speculation in the study. The polymerase chain reaction (PCR), in which DNA is exponentially amplified, is a common but effective tool in the molecular biology toolbox.

Many kits have been developed that use PCR or PCR-based techniques, including quantitative PCR (also known as qPCR or real-time PCR), reverse transcription PCR (RT-PCR), and quantitative reverse transcription PCR (RT- qPCR).

These handy kits generally contain all the essential ingredients needed for a successful PCR reaction, such as polymerase, deoxynucleotides (dNTPs), buffer, and magnesium chloride. Sequence-specific primer sets are generally included for PCR detection or assay kits.

These kits can serve as useful tools in applications such as amplification of templates for sequencing and cloning, library creation, forensic identification, mutagenesis, expression profiling, and amplification of multiple targets at once (multiplexing). The PCR kits have been optimized for specific conditions to avoid speculation in studies.

When selecting the appropriate PCR kits, it is important to consider the PCR medium used, the desired precision, the read length versus lead time, and the end application. Because PCR can be a complex and challenging process, it is important to work with a vendor who has excellent and readily available technical support.