Most current choices for breast cancer sufferers are made on the premise of prognostic and predictive factors. In addition to the standard tumor/nodal/metastasis staging variables, estrogen and progesterone receptor standing as assessed by biochemical ligand-binding assays are the one different factors which have been adequately validated and advisable for routine scientific use. Pathologists at the moment, nonetheless, are evaluating estrogen and progesterone receptors virtually solely by immunohistochemical means.
Although many research counsel that these exams may need equal and even superior talents to foretell affected person consequence, there are essential methodologic shortcomings to resolve earlier than this know-how achieves the scientific and technical validation essential to justify its routine use.
Many laboratories are additionally evaluating different factors for scientific use by immunohistochemical methods, together with, in specific, c-erbB-2, p53, and Ki-67 proliferation indices. Although obtainable research counsel that these factors may certainly be useful in making remedy choices, their scientific usefulness remains to be controversial, and, just like the evaluation of hormone receptors, there are essential unresolved technical points, similar to the way to put together the tissue, which reagents to make use of and, most significantly, the way to interpret the outcomes.
A number of laboratories have gone to appreciable effort to develop reproducible strategies for evaluating these factors, and they’ve carried out complete research demonstrating the prognostic and predictive significance of their outcomes. Nonetheless, most laboratories providing these exams haven’t adequately validated them and won’t even pay attention to the problems needing consideration.
Unless laboratories validate their exams or comply with the procedures of others who’ve, they run the chance of reporting meaningless and doubtlessly dangerous outcomes. In the longer term, these and different factors will likely be integrated right into a prognostic index that can higher replicate the biologic variety of breast cancer and that can extra precisely predict scientific consequence.
Structural rearrangements of tubulin and actin in the course of the cell cycle of the yeast Saccharomyces.
The distribution of actin and tubulin in the course of the cell cycle of the budding yeast Saccharomyces was mapped by immunofluorescence utilizing mounted cells from which the partitions had been eliminated by digestion. The intranuclear mitotic spindle was proven clearly by staining with a monoclonal antitubulin; the presence of in depth bundles of cytoplasmic microtubules is reported.
In cells containing quick spindles nonetheless solely throughout the mom cells, one of many bundles of cytoplasmic microtubules almost all the time prolonged to (or into) the bud. Two unbiased reagents (anti-yeast actin and fluorescent phalloidin) revealed an uncommon distribution of actin: it was current as a set of cortical dots or patches and additionally as distinct fibers that have been presumably bundles of actin filaments.
Double labeling confirmed that at no stage in the cell cycle do the distributions of actin and tubulin coincide for any vital size, and, in specific, that the mitotic spindle didn’t stain detectably for actin. However, each microtubule and actin staining patterns change in a attribute approach in the course of the cell cycle. In specific, the actin dots clustered in rings concerning the bases of very small buds and on the websites on unbudded cells at which bud emergence was apparently imminent.
Later in the budding cycle, the actin dots have been current largely in the buds and, in many strains, primarily on the suggestions of those buds. At concerning the time of cytokinesis the actin dots clustered in the neck area between the separating cells. These features of actin distribution counsel that it might have a job in the localized deposition of recent cell wall materials.
The NOD mouse: a mannequin of immune dysregulation.
Autoimmunity is a fancy course of that seemingly outcomes from the summation of a number of faulty tolerance mechanisms. The NOD mouse pressure is a wonderful mannequin of autoimmune illness and an essential device for dissecting tolerance mechanisms.
The power of this mouse pressure is that it develops spontaneous autoimmune diabetes, which shares many similarities to autoimmune or kind 1a diabetes (T1D) in human topics, together with the presence of pancreas-specific autoantibodies, autoreactive CD4+ and CD8+ T cells, and genetic linkage to illness syntenic to that discovered in people.
During the previous ten years, investigators have used all kinds of instruments to review these mice, together with immunological reagents and transgenic and knockout strains; these instruments have tremendously enhanced the examine of the elemental illness mechanisms. In addition, investigators have just lately developed a lot of therapeutic interventions in this animal mannequin which have now been translated into human therapies.
In this assessment, we summarize lots of the essential options of illness improvement and development in the NOD pressure, emphasizing the position of central and peripheral tolerance mechanisms that have an effect on diabetes in these mice. The data gained from this extremely related mannequin of human illness will result in potential therapies that will alter the event of the illness and its development in sufferers with T1D.
This assemble is lacking a number of the early and a lot of the late Ad genes and is incapable of manufacturing infectious Ad. Transfection of 293 cells with the brand new mini-Ad helper and AAV packaging plasmids outcomes in high-titer rAAV vectors with yields better than 1,000 transducing items, or 10(5) viral particles per cell.
ExFect2000 Transfection Reagent |
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T202-03 | Vazyme | 5 ml | 1262.4 EUR |
PureFection Transfection Reagent |
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LV750A-1 | SBI | 1 ml | 303 EUR |
iMFectin DNA Transfection Reagent |
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I7100-100 | GenDepot | 100ul | 176.4 EUR |
iMFectin DNA Transfection Reagent |
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I7100-101 | GenDepot | 1ml | 530.4 EUR |
iMFectin DNA Transfection Reagent |
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I7100-105 | GenDepot | 5x1ml | 2247.6 EUR |
iMFectin DNA Transfection Reagent |
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I7100-120 | GenDepot | 20ml | 8144.4 EUR |
GeneGlide? DNA Transfection Reagent |
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M1080-1000 | Biovision | each | 636 EUR |
GeneGlide? DNA Transfection Reagent |
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M1080-300 | Biovision | each | 322.8 EUR |
GeneGlide? DNA Transfection Reagent |
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M1080-500 | Biovision | each | 433.2 EUR |
Lentifectin (TM) Transfection Reagent |
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DNAF003 | Bio Basic | 1ml | 334.57 EUR |
Transfectamine™ mRNA Transfection Reagent |
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60030 | AAT Bioquest | 500 ul | 203 EUR |
Sapphire Insect Transfection Reagent |
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ABP-BVD-10003 | Allele Biotech | 75ul, 25 transfections | Ask for price |
GeneGlide? siRNA Transfection Reagent |
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M1081-1000 | Biovision | each | 534 EUR |
GeneGlide? siRNA Transfection Reagent |
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M1081-300 | Biovision | each | 318 EUR |
GeneGlide? siRNA Transfection Reagent |
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M1081-500 | Biovision | each | 364.8 EUR |
iMFectin 2000 DNA Transfection Reagent |
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I7300-101 | GenDepot | 1ml | Ask for price |
iMFectin 2000 DNA Transfection Reagent |
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I7300-105 | GenDepot | 5x1ml | Ask for price |
PureFection™ Transfection reagent |
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LV750A-5 | SBI | 5 ml | 1287 EUR |
T-Pro P-Fect Transfection Reagent |
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JT97-N005M | T-Pro Biotechnology | 1.0ml/vial | 266.4 EUR |
iMFectin Poly DNA Transfection Reagent |
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I7200-100 | GenDepot | 100ul | 176.4 EUR |
iMFectin Poly DNA Transfection Reagent |
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I7200-101 | GenDepot | 1ml | 530.4 EUR |
iMFectin Poly DNA Transfection Reagent |
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I7200-105 | GenDepot | 5x1ml | 2247.6 EUR |
iMFectin Poly DNA Transfection Reagent |
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I7200-120 | GenDepot | 20ml | 8144.4 EUR |
RNAfection, RNA transfection reagent 50 ul |
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MR750A-1 | SBI | 50 ul | 157 EUR |
T-Pro Nonliposomal Transfection Reagent I (NTR I) |
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JT97-N001M | T-Pro Biotechnology | 1.0ml/vial | 224.4 EUR |
T-Pro Nonliposomal Transfection Reagent II (NTR II) |
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JT97-N002M | T-Pro Biotechnology | 1.0ml/vial | 244.8 EUR |
T-Pro Nonliposomal Transfection Reagent III (NTR III) |
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JT97-N006M | T-Pro Biotechnology | 1.0ml/vial | 266.4 EUR |
pPACK-SPIKE Combo Kit, includes Cat# CVD19-500A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
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CVD19-520A-KIT | SBI | 1 kit | 1141 EUR |
pPACK-SPIKE D614G Combo Kit, includes Cat# CVD19-530A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
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CVD19-550A-KIT | SBI | 1 kit | 1141 EUR |
pPACK-SPIKE N501Y Combo Kit, includes Cat# CVD19-560A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
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CVD19-575A-KIT | SBI | 1 kit | 1141 EUR |
pPACK-SPIKE Alpha Combo Kit, includes Cat# CVD19-590A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
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CVD19-599A-KIT | SBI | 1 kit | 1141 EUR |
pPACK-SPIKE Gamma Combo Kit, includes Cat# CVD19-630A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
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CVD19-639A-KIT | SBI | 1 kit | 1141 EUR |
pPACK-SPIKE Beta Combo Kit, includes Cat# CVD19-640A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
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CVD19-649A-KIT | SBI | 1 kit | 1141 EUR |
pPACK-SPIKE Delta Combo Kit, includes Cat# CVD19-650A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
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CVD19-659A-KIT | SBI | 1 kit | 1141 EUR |
pPACK-SPIKE Omicron Combo Kit, includes Cat# CVD19-660A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
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CVD19-669A-KIT | SBI | 1 kit | 1162 EUR |
pPACK-SPIKE B.1.429 Combo Kit, includes Cat# CVD19-610A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
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CVD19-619A-KIT | SBI | 1 kit | 1141 EUR |
pPACK-SPIKE B.1.525 Combo Kit, includes Cat# CVD19-620A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
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CVD19-629A-KIT | SBI | 1 kit | 1141 EUR |
pPACK-SPIKE B.1.351 RBD Mutations Combo Kit, includes Cat# CVD19-580A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
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CVD19-589A-KIT | SBI | 1 kit | 1141 EUR |
pPACK-SPIKE B.1.351 S1 Mutations Combo Kit, includes Cat# CVD19-600A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
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CVD19-609A-KIT | SBI | 1 kit | 1141 EUR |
FBS, Transfection Optimized |
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F0640-050 | GenDepot | 500ml | 902.4 EUR |
293T Transfection Kit (1 mL) |
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P902 | 101Bio | - | Ask for price |
293T Transfection Kit (0.2 mL) |
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P902S | 101Bio | - | Ask for price |
293T Transfection Kit (1 mL) |
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P903 | 101Bio | - | Ask for price |
293T Transfection Kit (0.2 mL) |
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P903S | 101Bio | - | Ask for price |
Calcium Phosphate Transfection Kit |
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GR103070 | Genorise Scientific | 100 preps | 219 EUR |
Exo-Fect Exosome Transfection Kit |
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EXFT10A-1 | SBI | 10 reactions | 238 EUR |
Exo-Fect Exosome Transfection Kit |
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EXFT20A-1 | SBI | 20 reactions | 429 EUR |
Protoplast Solution for Transfection |
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GR103052 | Genorise Scientific | 10 mL | 119 EUR |
Exo-Fect siRNA/miRNA Transfection Kit |
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EXFT200A-1 | SBI | 20 reactions | 412 EUR |
NHEJ Based HeLa Requires I-Sce1 Transfection (100ug DNA) |
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DR5001-HNHela-Sce2 | TopoGen | 100ug DNA | 3486 EUR |
NHEJ Based HeLa Requires I-Sce1 Transfection (250ug DNA) |
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DR5001-HNHela-Sce3 | TopoGen | 250ug DNA | 4110 EUR |
Transfection-ready Cas9 SmartNuclease AAVS1 gRNA |
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CAS520A-1 | SBI | 10 ug | 281 EUR |
Transfection Collection™ : PDE4D Transient Pack |
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79286 | BPS Bioscience | 500 rxns. | 845 EUR |
Description: The PDE4D Transient Pack is designed to provide the tools necessary for transiently transfecting and for screening inhibitors of PDE4D7 in cultured HEK293 cells._x000D_The assay is based on transfecting cells with the CRE luciferase reporter. CRE reporter contains the firefly luciferase gene under the control of cAMP response element (CRE). Elevation of intracellular cAMP activates CRE binding protein (CREB) to bind CRE and induce the expression of luciferase. Forskolin is commonly used to raise the intracellular level of cAMP in cell physiology studies. When cells transiently transfected with CRE reporter are activated by forskolin, the intracellular level of cAMP is upregulated, which induces the expression of CRE luciferase reporter._x000D_However, when cells are co-transfected with PDE4D7 expression vector and CRE reporter, the level of forskolin-induced cAMP is reduced, resulting in lower expression level of luciferase. When cells are treated with PDE4D inhibitor to inhibit PDE4D7 activity, cAMP level is restored, resulting in higher luciferase activity. The PDE4D Transient Pack contains transfection-ready CRE luciferase reporter. This reporter contains transfection-ready vectors for PDE4D7 expression and a CRE vector containing firefly luciferase as a cAMP pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control._x000D_It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells. The key to the PDE4D Cell-Based Activity Reporter Detection Valuepack is the CRE luciferase reporter vector, which is a cAMP/PKA Cell Signaling Pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized cAMP response elements (CRE) located upstream of a minimal promoter. The vector is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency. The pack also includes the PDE4D7 expression vector, and the adenylate cyclase activator, forskolin. Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 and HeLa cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep._x000D_Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HEK293 or HeLa, but an alternate cell culture medium may be required for optimal cell growth |
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Sapphire Baculovirus DNA and Transfection Kit |
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ABP-BVD-10002 | Allele Biotech | 1 kit | Ask for price |
Phosphate-buffered Sucrose for Transfection |
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GR103050 | Genorise Scientific | 1 L | 119 EUR |
Plant Electroporation Buffer for Transfection |
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GR103051 | Genorise Scientific | 1 L | 119 EUR |
HR based Kit in HeLa Requiring CRISPR Transfection (100ug DNA) |
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DR3000-HRHeLa-CPR2 | TopoGen | 100ug DNA | 3610.8 EUR |
HR based Kit in HeLa Requiring CRISPR Transfection (250ug DNA) |
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DR3000-HRHeLa-CPR3 | TopoGen | 250ug DNA | 4234.8 EUR |
HR based Kit in HeLa Requiring I-Sce1 Transfection (100ug DNA) |
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DR3000-HRHeLa-Sce2 | TopoGen | 100ug DNA | 3486 EUR |
HR based Kit in HeLa Requiring I-Sce1 Transfection (250ug DNA) |
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DR3000-HRHeLa-Sce3 | TopoGen | 250ug DNA | 4110 EUR |
HR in human Neuroblastoma Cells req. I-Sce1 Transfection (100ug DNA) |
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DR3000-HRSH-Sce2 | TopoGen | 100ug DNA | 3735.6 EUR |
HR in human Neuroblastoma Cells req. I-Sce1 Transfection (250ug DNA) |
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DR3000-HRSH-Sce3 | TopoGen | 250ug DNA | 4359.6 EUR |
NHEJ Based Mouse 3T3 cells Requires I-Sce1 Transfection (100ug DNA) |
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DR5000-HN3T3-Sce2 | TopoGen | 100ug DNA | 3486 EUR |
NHEJ Based Mouse 3T3 cells Requires I-Sce1 Transfection (250ug DNA) |
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DR5000-HN3T3-Sce3 | TopoGen | 250ug DNA | 4110 EUR |
"S" SARS-CoV-2 Spike Omicron mRNA (10 µg, Transfection-ready) |
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CVD19-440A-1 | SBI | 10 µg | 383 EUR |
"S" SARS-CoV-2 Spike Omicron mRNA (20 µg, Transfection-ready) |
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CVD19-445A-1 | SBI | 20 µg | 553 EUR |
100 x DEAE-dextran Stock Solution for Transfection |
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GR103049 | Genorise Scientific | 10 x 10 mL | 119 EUR |
"N" SARS-CoV-2 Nucleocapsid mRNA (10 µg, Transfection-ready) |
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CVD19-420A-1 | SBI | 10 µg | 328 EUR |
"N" SARS-CoV-2 Nucleocapsid mRNA (20 µg, Transfection-ready) |
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CVD19-425A-1 | SBI | 20 µg | 468 EUR |
NHEJ Based HeLa Requires I-Sce1 Transfection (50ug DNA) |
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DR5001-HNHela-Sce | TopoGen | 50ug DNA | 3174 EUR |
HR based Kit in MOUSE 3T3 Cells Requiring I-Sce1 Transfection (100ug DNA) |
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DR3000-HR3T3-Sce2 | TopoGen | 100ug DNA | 3486 EUR |
HR based Kit in MOUSE 3T3 Cells Requiring I-Sce1 Transfection (250ug DNA) |
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DR3000-HR3T3-Sce3 | TopoGen | 250ug DNA | 4110 EUR |
Transfection-ready Cas9 SmartNuclease mRNA (Eukaryotic version) |
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CAS500A-1 | SBI | 20 ug | 387 EUR |
HR based Kit in HeLa Requiring CRISPR Transfection (50ug DNA) |
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DR3000-HRHeLa-CPR | TopoGen | 50ug DNA | 3298.8 EUR |
HR based Kit in HeLa Requiring I-Sce1 Transfection (50ug DNA) |
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DR3000-HRHeLa-Sce | TopoGen | 50ug DNA | 3174 EUR |
"S" SARS-CoV-2 Spike Glycoprotein mRNA (10 µg, Transfection-ready) |
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CVD19-430A-1 | SBI | 10 µg | 360 EUR |
"S" SARS-CoV-2 Spike Glycoprotein mRNA (20 µg, Transfection-ready) |
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CVD19-435A-1 | SBI | 20 µg | 520 EUR |
HR based Kit in Mouse Neuronal Cells Requiring I-Sce1 Transfection (100ug DNA) |
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DR3000-HRCN1.4-Sce2 | TopoGen | 100ug DNA | 3735.6 EUR |
HR based Kit in Mouse Neuronal Cells Requiring I-Sce1 Transfection (250ug DNA) |
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DR3000-HRCN1.4-Sce3 | TopoGen | 250ug DNA | 4359.6 EUR |
NHEJ Based in Mouse Neuronal CN1.4 cells requires I-Sce1 transfection (100ug DNA) |
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DR5000-HNCN1.4-Sce2 | TopoGen | 100ug DNA | 3486 EUR |
NHEJ Based in Mouse Neuronal CN1.4 cells requires I-Sce1 transfection (250ug DNA) |
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DR5000-HNCN1.4-Sce3 | TopoGen | 250ug DNA | 4110 EUR |
Transfection Collection™ : AP1 Transient Pack (JNK Signaling Pathway) |
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79266 | BPS Bioscience | 500 rxns. | 715 EUR |
Description: The AP1 Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the JNK signaling pathway and the transcriptional activity of AP1 in cultured HEK293 cells. The kit contains transfection-ready vectors containing firefly luciferase as a JNK pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the AP1 Transient Pack is the AP1 luciferase reporter vector. This reporter contains the firefly luciferase gene under the control of multimerized AP1 responsive elements located upstream of a minimal promoter. The AP1 reporter is premixed with a constitutively-expressing Renilla luciferase vector that serves as an internal control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively- expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical for determining pathway-specific effects and the background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 and HeLa cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required. _x000D_*Note: the kit may be used with other cell lines than HEK293 or HeLa, but an alternate cell culture medium may be required for optimal cell growth |
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Transfection Collection™ : Myc Transient Pack (Myc Signaling Pathway) |
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79284 | BPS Bioscience | 500 rxns. | 750 EUR |
Description: The Myc Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the Myc signaling pathway in cultured HCT116 cells. The Myc Transient Pack contains transfection-ready vectors containing firefly luciferase as a Myc pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HCT116 cells._x000D_.The key to the Myc Transient Pack is the expression vectors for c-Myc and Myc luciferase reporter vector. Inside the cells, c-Myc will bind to Max, translocate to the nucleus, and induce expression of the Myc luciferase reporter vector. This reporter contains the firefly luciferase gene under the control of multimerized Myc responsive elements located upstream of a minimal promoter. The Myc reporter is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. This negative control is critical for determining pathway-specific effects and background luciferase activity. An "empty" expression vector without the c-Myc gene is also provided as an additional negative control._x000D_Additionally, the pack includes cell culture medium that has been optimized for use with HCT116 cells*. HCT116 is a human colon cancer cell line with a mutated β -catenin which leads to the accumulation of β-catenin and constitutive activation of the Myc signaling pathway. This medium includes 10% fetal bovine serum, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HCT116, but an alternate cell culture medium may be required for optimal cell growth. For HEK293 cells, we recommend using BPS Medium 1 (BPS Bioscience, #79259). |
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NHEJ Based in Human Neuroblastoma Line requires I-Sce1 transfection (100ug DNA) |
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DR5000-HNSH-Sce2 | TopoGen | 100ug DNA | 3610.8 EUR |
NHEJ Based in Human Neuroblastoma Line requires I-Sce1 transfection (250ug DNA) |
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DR5000-HNSH-Sce3 | TopoGen | 250ug DNA | 4234.8 EUR |
HR in human Neuroblastoma Cells req. I-Sce1 Transfection (50ug DNA) |
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DR3000-HRSH-Sce | TopoGen | 50ug DNA | 3423.6 EUR |
NHEJ Based Mouse 3T3 cells Requires I-Sce1 Transfection (50ug DNA) |
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DR5000-HN3T3-Sce | TopoGen | 50ug DNA | 3174 EUR |
Transfection Collection™ : SRE Transient Pack (MAPK/ERK Signaling Pathway) |
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79271 | BPS Bioscience | 500 rxns. | 750 EUR |
Description: The SRE Reporter Kit Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the ERK signaling pathway and the transcriptional activity of SRE in cultured HEK293 cells. The kit contains transfection-ready vectors containing firefly luciferase as a MAPK/ERK pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the SRE Transient Pack is the SRE luciferase reporter vector, which is an ERK pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized SRE responsive elements located upstream of a minimal promoter. The SRE reporter is premixed with a constitutively-expressing Renilla luciferase vector that serves as an internal control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively- expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical for determining pathway-specific effects and the background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_* Other mammalian cell lines may also be used, but an alternate cell culture medium may be required for optimal cell growth. |
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HR based Kit in MOUSE 3T3 Cells Requiring I-Sce1 Transfection (50ug DNA) |
|||
DR3000-HR3T3-Sce | TopoGen | 50ug DNA | 3174 EUR |
Transfection Collection™ : NF-κB Transient Pack ( NF-κB Signaling Pathway) |
|||
79268 | BPS Bioscience | 500 rxns. | 780 EUR |
Description: The NF-κB Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the NF-κB signaling pathway in cultured HEK293 cells. The kit contains transfection-ready vectors containing firefly luciferase as a NF-κB pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the NF-κB Transient Pack is the NF-κB luciferase reporter vector. This reporter contains a firefly luciferase gene under the control of multimerized NF-κB responsive element located upstream of a minimal promoter. The NF-κB reporter is premixed with constitutively-expressing Renilla luciferase vector, which serves as an internal control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth. |
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Transfection Collection™ : SBE Transient Pack (TGFβ/SMAD Signaling Pathway) |
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79272 | BPS Bioscience | 500 rxns. | 750 EUR |
Description: The SBE Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of TGFβ/SMAD signaling pathway in the cultured cells. The kit contains transfection-ready vectors containing firefly luciferase as a TGFβ/SMAD pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the SBE Transient Pack is the SBE luciferase reporter vector, which is a TGFβ pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized SBE responsive element located upstream of a minimal promoter. The SBE reporter is premixed with constitutively expressing Renilla luciferase vector that serves as internal control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively expressing Renilla luciferase vector as negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required. _x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth. |
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Transfection-ready Cas9 SmartNickase mRNA (Eukaryotic Nickase mutant version) |
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CAS504A-1 | SBI | 20 ug | 387 EUR |
The Transfection Collection™ : ISRE Transient Pack JAK/STAT Signaling Pathway |
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79264 | BPS Bioscience | 500 rxns. | 715 EUR |
Description: The ISRE Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of Type I interferon-induced JAK/STAT signaling pathway in cultured HEK293 cells. The pack contains transfection-ready vectors containing firefly luciferase as a JAK/STAT pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the ISRE Transient Pack is the ISRE luciferase reporter vector, which is a JAK/STAT pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized ISRE responsive element located upstream of a minimal promoter. The ISRE reporter is premixed with constitutively-expressing Renilla luciferase vector, which serves as an internal control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth. |
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Transfection Collection™ : Notch1/CSL Transient Pack (Notch Signaling Pathway) |
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79285 | BPS Bioscience | 500 rxns. | 715 EUR |
Description: The Notch1/CSL Transient Packis designed to provide the tools necessary for transiently transfecting and monitoring the activity of the Notch signaling pathway in HEK293 cultured cells. The kit contains transfection-ready vectors containing firefly luciferase as a Notch pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the Notch1/CSL Transient Pack is the expression vector for NOTCH1 that has a deletion of the entire extracellular domain (Notch1DE). Inside the cells, the NOTCH1 DE can be cleaved by γ-secretase and active NOTCH1 NICD is released into the nucleus. The kit also contains CSL (CBF1/RBP-Jk) luciferase reporter vector, which is a Notch pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized CSL responsive elements upstream of a minimal promoter. The CSL (CBF1/RBP-Jk) reporter is premixed with constitutively expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required. _x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth, |
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HR based Kit in Mouse Neuronal Cells Requiring I-Sce1 Transfection (50ug DNA) |
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DR3000-HRCN1.4-Sce | TopoGen | 50ug DNA | 3423.6 EUR |
ExoFectin Plasmid DNA-into-Exosome Kit (DNA transfection kit for exosome) |
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P402 | 101Bio | - | Ask for price |
NHEJ Based in Mouse Neuronal CN1.4 cells requires I-Sce1 transfection (50ug DNA) |
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DR5000-HNCN1.4-Sce | TopoGen | 50ug DNA | 3174 EUR |
Transfection Collection™ : CRE/CREB Transient Pack (cAMP/PKA Cell Signaling Pathway) |
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79267 | BPS Bioscience | 500 rxns. | 715 EUR |
Description: The CRE/CREB Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the cAMP/PKA signaling pathway in cultured cells. The kit contains transfection-ready vectors containing firefly luciferase as a cAMP/PKA pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the CRE/CREB Transient Pack is the CRE/CREB luciferase reporter vector, which is a cAMP/PKA Cell Signaling Pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized cAMP response elements (CRE) located upstream of a minimal promoter. Elevation of the intracellular cAMP level activates cAMP response element binding protein (CREB) to bind CRE and induces the expression of luciferase._x000D_The CRE reporter is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with the constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_* Other mammalian cell lines may also be used, but an alternate cell culture medium may be required for optimal cell growth. |
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NHEJ Based in Human Neuroblastoma Line requires I-Sce1 transfection (50ug DNA) |
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DR5000-HNSH-Sce | TopoGen | 50ug DNA | 3298.8 EUR |
Transfection Collection™ : TCF/LEF Transient Pack Wnt / β-catenin Signaling Pathway |
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79273 | BPS Bioscience | 500 rxns. | 750 EUR |
Description: The TCF/LEF Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of Wnt / Beta-catenin signaling pathway in the cultured HEK293 cells. The TCF/LEF Transient Pack contains transfection-ready vectors containing firefly luciferase as a Wnt pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the TCF/LEF Transient Pack is the TCF/LEF luciferase reporter vector, which is a Wnt pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized TCF/LEF responsive element located upstream of a minimal promoter. The TCF/LEF reporter is premixed with constitutively expressing Renilla luciferase vector that serves as internal control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively expressing Renilla luciferase vector as negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth. |
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The Transfection Collection™ : GAL4 Transient Pack Glucocorticoid Receptor Pathway |
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79265 | BPS Bioscience | 500 rxns. | 715 EUR |
Description: The GAL4 Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the glucocorticoid signaling pathway in cultured HEK293 cells. The kit contains transfection-ready vectors containing firefly luciferase as a glucocorticoid pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the GAL4 Transient Pack is the expression vector for the glucocorticoid receptor ligand binding domain that is fused to the DNA binding domain (DBD) of GAL4 (GAL4 DBD-GR). This fusion construct activates firefly luciferase expression under the control of a multimerized GAL4 upstream activation sequence (UAS). This allows for specific detection of glucocorticoid-induced activation of the glucocorticoid receptor without the need for individual transcriptional targets and with low cross-reactivity for other nuclear receptor pathways. The GAL4/UAS reporter is premixed with constitutively expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway-specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth. |
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Transfection-ready hspCas9-T2A-GFP SmartNuclease mRNA (wildtype Cas9 with GFP marker) |
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CAS530G-1 | SBI | 10 ug | 342 EUR |
Transfection-ready hspCas9-T2A-RFP SmartNuclease mRNA (wildtype Cas9 with RFP marker) |
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CAS531R-1 | SBI | 10 ug | 342 EUR |
Transfection-ready hspCas9-nickase-T2A-GFP SmartNickase mRNA (Cas9 Nickase mutant with GFP marker) |
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CAS534G-1 | SBI | 10 ug | 342 EUR |
Transfection-ready hspCas9-nickase-T2A-RFP SmartNickase mRNA (Cas9 Nickase mutant with RFP marker) |
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CAS535R-1 | SBI | 10 ug | 342 EUR |
Sample Transport Reagent |
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SSFIN-0007 | Zeptometrix | 20mL | 32 EUR |
TransDux virus transduction reagent (200x) |
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LV850A-1 | SBI | 80 transductions | 257 EUR |
EP Reagent Biuret Reagent |
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1011601 | Scientific Laboratory Supplies | 1L | 52.8 EUR |
Traut's Reagent |
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2330-1000 | Biovision | each | 418.8 EUR |
Traut's Reagent |
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2330-500 | Biovision | each | 248.4 EUR |
EP Reagent Iodoplatinate Reagent |
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1046300 | Scientific Laboratory Supplies | 200ML | 446.4 EUR |
EP Reagent Molybdovanadic Reagent |
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1056700 | Scientific Laboratory Supplies | 100ML | 52.8 EUR |
EP Reagent Sulfomolybdic Reagent R3 |
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1086500 | Scientific Laboratory Supplies | 1L | 354 EUR |
EP Reagent Methoxyphenylacetic Reagent |
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1053601 | Scientific Laboratory Supplies | 100ML | 435.6 EUR |
EP Reagent Phosphomolybdotungstic Reagent |
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1065000 | Scientific Laboratory Supplies | 100ML | 201.6 EUR |
BOP reagent |
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5-02141 | CHI Scientific | 25g | Ask for price |
BOP reagent |
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5-02142 | CHI Scientific | 100g | Ask for price |
MTS Reagent |
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2808-1000 | Biovision | each | 1188 EUR |
MTS Reagent |
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2808-250 | Biovision | each | 438 EUR |
MTT Reagent |
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2809-1G | Biovision | each | 216 EUR |
MTT Reagent |
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2809-5G | Biovision | each | 652.8 EUR |
BOP reagent |
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A7015-100000 | ApexBio | 100 g | 240 EUR |
Description: A peptide coupling reagent. Can be used in the preparation of phenyl esters of amino acids which have been shown to be valuable as blocked derivatives of amino acids in the field of peptide synthesis. |
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BOP reagent |
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A7015-25000 | ApexBio | 25 g | 135.6 EUR |
Description: A peptide coupling reagent. Can be used in the preparation of phenyl esters of amino acids which have been shown to be valuable as blocked derivatives of amino acids in the field of peptide synthesis. |
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Cod Reagent 0-15000Ppm |
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24159-51 | Scientific Laboratory Supplies | PK25 | 116.4 EUR |
COD Reagent (1977 method) |
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WTR50W | Scientific Laboratory Supplies | 2.5L | 135.6 EUR |
DMPO (Spin trapping reagent) |
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SIH-324-125MG | Stressmarq | 125 mg | 255.6 EUR |
Description: The substance DMPO is a spin trapping reagent. It is synthetically produced and has a purity of >98%. The pure substance is liquid which is soluble to 100 mM in ethanol and to 100 mM in DMSO. |
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DMPO (Spin trapping reagent) |
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SIH-324-25MG | Stressmarq | 25 mg | 117.6 EUR |
Description: The substance DMPO is a spin trapping reagent. It is synthetically produced and has a purity of >98%. The pure substance is liquid which is soluble to 100 mM in ethanol and to 100 mM in DMSO. |
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Bluing Reagent |
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BRT030 | ScyTek Laboratories | 30 ml | 72 EUR |
Bluing Reagent |
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BRT125 | ScyTek Laboratories | 125 ml | 75.6 EUR |
Bluing Reagent |
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BRT3800 | ScyTek Laboratories | 1 Gal. | 220.8 EUR |
Bluing Reagent |
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BRT500 | ScyTek Laboratories | 500 ml | 91.2 EUR |
Bluing Reagent |
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BRT999 | ScyTek Laboratories | 1000 ml | 105.6 EUR |
ECL Start Reagent 2000cm2 |
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RPN3243 | Scientific Laboratory Supplies | EACH | 138 EUR |
ECL Start Reagent 4000cm2 |
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RPN3244 | Scientific Laboratory Supplies | EACH | 222 EUR |
BURGESS REAGENT |
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702010 | Survival Technologies | each | Ask for price |
Chymase reagent |
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30C-CP1129 | Fitzgerald | 5 units | 2622 EUR |
Description: Purified native Human Chymase reagent |
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Nessler Reagent |
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NESSR | Scientific Laboratory Supplies | 500ML | 182.4 EUR |
Chromium Reagent |
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1206699 | Scientific Laboratory Supplies | EACH | 157.2 EUR |
Bradford reagent |
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BDE641 | Bio Basic | 100ml | 73.21 EUR |
Beaucage reagent |
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HY-100951 | MedChemExpress | 10mM/1mL | 151.2 EUR |
Phosphate Reagent |
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106199 | Scientific Laboratory Supplies | PK100 | 69.6 EUR |
Ninhydrin Reagent |
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MIC6746 | Scientific Laboratory Supplies | EACH | 31.12 EUR |
ECL Start Reagent 200cm2 |
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RPN3245 | Scientific Laboratory Supplies | EACH | 26.4 EUR |
BCA Reagent, 16ML |
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C144-16ML | Arbor Assays | 16ML | 132 EUR |
TDA Reagent - 10mL |
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MIC6732 | Scientific Laboratory Supplies | EACH | 26.33 EUR |
PYR Reagent - 10mL |
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MIC6744 | Scientific Laboratory Supplies | EACH | 31.12 EUR |
Bottle Reagent 1000 ml Pyrex |
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BOT5356 | Scientific Laboratory Supplies | PK10 | 177.6 EUR |
Bottle Reagent 500ml Pyrex |
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B54564 | Scientific Laboratory Supplies | PK10 | 124.8 EUR |
Silica 3 Reagent |
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27169 | Scientific Laboratory Supplies | 100ML | 99.6 EUR |
BSA (Reagent Grade) |
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30-AB79 | Fitzgerald | 1 kg | 1862.4 EUR |
Description: Reagent Grade Bovine Serum Albumin (99% pure) |
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BSA (Reagent Grade) |
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30-AB81 | Fitzgerald | 200 grams | 571.2 EUR |
Description: Reagent Grade Sulphydryl Blocked BSA (99% pure) |
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Girard's reagent T |
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20-abx184099 | Abbexa |
|
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Tri-RNA Reagent |
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FATRR-001 | Favorgen | 100ml | 283.2 EUR |
Tri-RNA Reagent |
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FATRR-002 | Favorgen | 50ml | 211.2 EUR |
Tri-RNA Reagent |
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FATRR-003 | Favorgen | 450ml | 774 EUR |
Biotin reagent (HRP) |
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65C-CE0202 | Fitzgerald | 5 mg | 292.8 EUR |
Description: HRP conjugated biotin labelling reagent |
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EP Reagent Ferroin |
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1038100 | Scientific Laboratory Supplies | 100ML | 96 EUR |
Griess Reagent Kit |
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30100 | Biotium | 1KIT | 178.8 EUR |
Description: Minimum order quantity: 1 unit of 1KIT |
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Alcohol, Reagent (70%) |
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EAS500 | ScyTek Laboratories | 500 ml | 94.8 EUR |
Alcohol, Reagent (70%) |
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EAS999 | ScyTek Laboratories | 1000 ml | 121.2 EUR |
EZ Cap? Reagent AG |
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B8176-.01 | ApexBio | 10 µl (100 mM) | 369.6 EUR |
Description: EZ Cap Reagent AG is a co-transcriptional capping reagent for in vitro transcription of 5? capped mRNA which would result in a Cap 1 structure. Cap AG has shown to provide up to 98% capping efficiency in literatures.EZ Cap AG requires an AG initiator. |
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EZ Cap? Reagent AG |
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B8176-.05 | ApexBio | 50 µl (100 mM) | 1488 EUR |
Description: EZ Cap Reagent AG is a co-transcriptional capping reagent for in vitro transcription of 5? capped mRNA which would result in a Cap 1 structure. Cap AG has shown to provide up to 98% capping efficiency in literatures.EZ Cap AG requires an AG initiator. |
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EZ Cap? Reagent AG |
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B8176-.1 | ApexBio | 100 µl (100 mM) | 2656.8 EUR |
Description: EZ Cap Reagent AG is a co-transcriptional capping reagent for in vitro transcription of 5? capped mRNA which would result in a Cap 1 structure. Cap AG has shown to provide up to 98% capping efficiency in literatures.EZ Cap AG requires an AG initiator. |
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EZ Cap? Reagent GG |
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B8177-.01 | ApexBio | 10 µl (100 mM) | 369.6 EUR |
Description: EZ Cap Reagent GG is a co-transcriptional capping reagent for in vitro transcription of 5? capped mRNA resulting in a Cap 1 structure. EZ Cap GG requires a GG initiator. EZ Cap GG typically provides >90% capped material generating a natural Cap 1 structure. |
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EZ Cap? Reagent GG |
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B8177-.05 | ApexBio | 50 µl (100 mM) | 1488 EUR |
Description: EZ Cap Reagent GG is a co-transcriptional capping reagent for in vitro transcription of 5? capped mRNA resulting in a Cap 1 structure. EZ Cap GG requires a GG initiator. EZ Cap GG typically provides >90% capped material generating a natural Cap 1 structure. |
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Thioacetamide Reagent |
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THIOR01 | Scientific Laboratory Supplies | 100ML | 99.6 EUR |
When rAAV vectors have been produced by utilizing this manufacturing scheme and in comparison with conventional heat-inactivated rAAV preparations in vitro and in vivo, we noticed transduction equal to or higher than regular ranges. The full removing of infectious Ad from AAV manufacturing ought to facilitate a greater understanding of immune response to AAV vectors in vivo, eradicate the necessity for creating replication-competent Ad assays, and present a extra outlined reagent for scientific use.