Rabbit polyclonal antibody (immunoglobulin) is produced in an animal that is able to produce antibodies against many antigens. Rabbit monoclonal antibody (MAb) has become popular in the past decade, but for most applications rabbit polyclonal antibodies are still used because of their lower cost and wider availability. For certain purposes, other sources may be more convenient or even better than rabbits despite possibly shorter supply or greater cost. Rabbits should only be used when the non-specific background staining is not prohibitive; thus care must be taken to avoid nonspecific absorption effects which make it difficult to interpret findings.
The main advantages of using polyclonal are: they are cheap, and they are more likely to bind a wider range of epitopes on the antigen than monoclonal antibodies. The disadvantages are that they are not as specific as monoclonal antibodies and they can cause nonspecific background staining.
Rabbit Antibody
MBS5680282-10mg
MyBiosource
10mg
Rabbit Antibody
MBS5680282-5x10mg
MyBiosource
5x10mg
Rabbit Polyclonal Antibody PC4 Rabbit Polyclonal Antibody
MBS9465999-005mL
MyBiosource
0.05mL
Rabbit Polyclonal Antibody PC4 Rabbit Polyclonal Antibody
MBS9465999-01mL
MyBiosource
0.1mL
Rabbit Polyclonal Antibody PC4 Rabbit Polyclonal Antibody
MBS9465999-5x01mL
MyBiosource
5x0.1mL
Rabbit Polyclonal Antibody PC1 Rabbit Polyclonal Antibody
MBS9465163-005mL
MyBiosource
0.05mL
Rabbit Polyclonal Antibody PC1 Rabbit Polyclonal Antibody
MBS9465163-01mL
MyBiosource
0.1mL
Rabbit Polyclonal Antibody PC1 Rabbit Polyclonal Antibody
MBS9465163-5x01mL
MyBiosource
5x0.1mL
ATM Rabbit Rabbit Polyclonal Antibody
ES8456-100ul
ELK Biotech
100ul
ATM Rabbit Rabbit Polyclonal Antibody
ES8456-50ul
ELK Biotech
50ul
ATM Rabbit Rabbit Polyclonal Antibody
ES8457-100ul
ELK Biotech
100ul
ATM Rabbit Rabbit Polyclonal Antibody
ES8457-50ul
ELK Biotech
50ul
MYC Rabbit Rabbit Monoclonal Antibody
E46F2292
EnoGene
40ul
VEGF Rabbit Rabbit Polyclonal Antibody
ES8453-100ul
ELK Biotech
100ul
VEGF Rabbit Rabbit Polyclonal Antibody
ES8453-50ul
ELK Biotech
50ul
CD10 Rabbit Rabbit Polyclonal Antibody
ES8454-100ul
ELK Biotech
100ul
CD10 Rabbit Rabbit Polyclonal Antibody
ES8454-50ul
ELK Biotech
50ul
JAK1 Rabbit Rabbit Polyclonal Antibody
ES8562-100ul
ELK Biotech
100ul
JAK1 Rabbit Rabbit Polyclonal Antibody
ES8562-50ul
ELK Biotech
50ul
JAK2 Rabbit Rabbit Polyclonal Antibody
ES8563-100ul
ELK Biotech
100ul
JAK2 Rabbit Rabbit Polyclonal Antibody
ES8563-50ul
ELK Biotech
50ul
JNK2 Rabbit Rabbit Polyclonal Antibody
ES8564-100ul
ELK Biotech
100ul
JNK2 Rabbit Rabbit Polyclonal Antibody
ES8564-50ul
ELK Biotech
50ul
JNK3 Rabbit Rabbit Polyclonal Antibody
ES8565-100ul
ELK Biotech
100ul
JNK3 Rabbit Rabbit Polyclonal Antibody
ES8565-50ul
ELK Biotech
50ul
MEK2 Rabbit Rabbit Polyclonal Antibody
ES8566-100ul
ELK Biotech
100ul
MEK2 Rabbit Rabbit Polyclonal Antibody
ES8566-50ul
ELK Biotech
50ul
MEK3 Rabbit Rabbit Polyclonal Antibody
ES8567-100ul
ELK Biotech
100ul
MEK3 Rabbit Rabbit Polyclonal Antibody
ES8567-50ul
ELK Biotech
50ul
Nrf2 Rabbit Rabbit Polyclonal Antibody
ES8568-100ul
ELK Biotech
100ul
Nrf2 Rabbit Rabbit Polyclonal Antibody
ES8568-50ul
ELK Biotech
50ul
ATG5 Rabbit Rabbit Polyclonal Antibody
ES8572-100ul
ELK Biotech
100ul
ATG5 Rabbit Rabbit Polyclonal Antibody
ES8572-50ul
ELK Biotech
50ul
ATG7 Rabbit Rabbit Polyclonal Antibody
ES8573-100ul
ELK Biotech
100ul
ATG7 Rabbit Rabbit Polyclonal Antibody
ES8573-50ul
ELK Biotech
50ul
NBR1 Rabbit Rabbit Polyclonal Antibody
ES8578-100ul
ELK Biotech
100ul
NBR1 Rabbit Rabbit Polyclonal Antibody
ES8578-50ul
ELK Biotech
50ul
NBR1 Rabbit Rabbit Polyclonal Antibody
ES8579-100ul
ELK Biotech
100ul
NBR1 Rabbit Rabbit Polyclonal Antibody
ES8579-50ul
ELK Biotech
50ul
Gab1 Rabbit Rabbit Polyclonal Antibody
ES8582-100ul
ELK Biotech
100ul
Gab1 Rabbit Rabbit Polyclonal Antibody
ES8582-50ul
ELK Biotech
50ul
ERK1 Rabbit Rabbit Polyclonal Antibody
ES8583-100ul
ELK Biotech
100ul
ERK1 Rabbit Rabbit Polyclonal Antibody
ES8583-50ul
ELK Biotech
50ul
MUC1 Rabbit Rabbit Monoclonal Antibody
E46F2310
EnoGene
40ul
MUC4 Rabbit Rabbit Monoclonal Antibody
E46F2353
EnoGene
40ul
NFE2 Rabbit Rabbit Monoclonal Antibody
E46F2371
EnoGene
40ul
NPC1 Rabbit Rabbit Monoclonal Antibody
E46F2391
EnoGene
40ul
MICA Rabbit Rabbit Monoclonal Antibody
E46F2396
EnoGene
40ul
NM23A Rabbit Rabbit Polyclonal Antibody
ES8455-100ul
ELK Biotech
100ul
NM23A Rabbit Rabbit Polyclonal Antibody
ES8455-50ul
ELK Biotech
50ul
Rabbit polyclonal antibody preparations are usually made by immunizing rabbits with the antigen to be detected, and then harvesting serum from the animals. The serum is then screened for reactivity with the antigen in a variety of formats including ELISA, Western blotting, and immunohistochemistry. Selected sera are then fractionated and tested for purity. The final product is a concentrated solution of rabbit IgG (immunoglobulin G) that is ready for use in research or diagnostic assays.
Rabbit polyclonal antibodies can be used in a variety of applications, including:
• Western blotting
• Immunohistochemistry (IHC)
• ELISA
• Immunoprecipitation
• In situ hybridization (ISH)
• Flow cytometry
• Chromatin immunoprecipitation (ChIP)
etc.
Today, most monoclonal antibodies are generated in mice or rats, but rabbits are still commonly used to generate polyclonal antibody preparations. The main advantage of using rabbits is that they can be immunized with a wider range of antigens than other animals. Additionally, the cost of producing rabbit polyclonal antibodies is lower than for monoclonal antibodies, making them a more affordable option for many research applications. The disadvantages of using rabbit polyclonal antibodies are that they are less specific than monoclonal antibodies and can cause nonspecific background staining. However, when used with caution, rabbit polyclonal antibodies can be a valuable tool for researchers and clinicians.
What is Rabbit Antibody?
Rabbit polyclonal antibody (immunoglobulin) is produced in an animal that is able to produce antibodies against many antigens. For certain purposes, other sources may be more convenient or even better than rabbits despite possibly shorter supply or greater cost. Rabbits should only be used when the non-specific background staining is not prohibitive; thus care must be taken to avoid nonspecific absorption effects which make it difficult to interpret findings.
The main advantages of using polyclonal are: they are cheap, and they are more likely to bind a wider range of epitopes on the antigen than monoclonal antibodies. The disadvantages are that they are not as specific as monoclonal antibodies and they can cause nonspecific background staining.
Rabbit polyclonal antibody preparations are usually made by immunizing rabbits with the antigen to be detected, and then harvesting serum from the animals. The serum is then screened for reactivity with the antigens in a variety of formats including ELISA, Western blotting, and immunohistochemistry. Selected sera are then fractionated and tested for purity. The final product is a concentrated solution of rabbit IgG (immunoglobulin G) that is ready for use in research or diagnostic assays.
Rabbit polyclonal antibodies can be used in a variety of applications, including:
western blotting, immunohistochemistry (IHC), ELISA, immunoprecipitation, in situ hybridization (ISH), flow cytometry, chromatin immunoprecipitation (ChIP), etc.
Today, most monoclonal antibodies are generated in mice or rats, but rabbits are still commonly used to generate polyclonal antibody preparations. The main advantage of using rabbits is that they can be immunized with a wider range of antigens than other animals. Additionally, the cost of producing rabbit polyclonal antibodies is lower than for monoclonal antibodies, making them a more affordable option for many research applications. The disadvantages of using rabbit polyclonal antibodies are that they are less specific than monoclonal antibodies and can cause nonspecific background staining. However, when used with caution, rabbit polyclonal antibodies can be a valuable tool for researchers and clinicians.
“Rabbit antibody (immunoglobulin) is produced in an animal that is able to produce antibodies against many antigens.” For certain purposes, other sources may be more convenient or even better than rabbits despite possibly shorter supply or greater cost.