Rabbit polyclonal antibody (immunoglobulin) is produced in an animal that is able to produce antibodies against many antigens. Rabbit monoclonal antibody (MAb) has become popular in the past decade, but for most applications rabbit polyclonal antibodies are still used because of their lower cost and wider availability. For certain purposes, other sources may be more convenient or even better than rabbits despite possibly shorter supply or greater cost. Rabbits should only be used when the non-specific background staining is not prohibitive; thus care must be taken to avoid nonspecific absorption effects which make it difficult to interpret findings.
The main advantages of using polyclonal are: they are cheap, and they are more likely to bind a wider range of epitopes on the antigen than monoclonal antibodies. The disadvantages are that they are not as specific as monoclonal antibodies and they can cause nonspecific background staining.
Anti-Dnmt3b Rabbit Monoclonal Antibody Rabbit Monoclonal Antibody
M00319
BosterBio
100ug/vial
Rabbit Tpa Antibody
abx023929-02mg
Abbexa
0.2 mg
Rabbit VWCE antibody
orb5385
Biorbyt
200ug
Rabbit Anti FITC Antibody
E61I01402
EnoGene
100ug
Rabbit Albumin Antibody
40150-05011
AssayPro
150 ug
IBA1 Polyclonal Rabbit Antibody
RA22133
Neuromics
100 ul
Rabbit IgG Fc Antibody
R20173-100UG
NSJ Bioreagents
100ug
Rabbit IgG Fc Antibody
R20173BTN-50UG
NSJ Bioreagents
50ug
Rabbit IgG Fc Antibody
R20173SAF-250UG
NSJ Bioreagents
250ug
p40(Rabbit PAb) Antibody
BNCA0375-250
Biotium
250uL
p40(Rabbit PAb) Antibody
BNC800375-100
Biotium
100uL
p40(Rabbit PAb) Antibody
BNC800375-500
Biotium
500uL
p40(Rabbit PAb) Antibody
BNC810375-100
Biotium
100uL
p40(Rabbit PAb) Antibody
BNC810375-500
Biotium
500uL
p40(Rabbit PAb) Antibody
BNC940375-100
Biotium
100uL
p40(Rabbit PAb) Antibody
BNC940375-500
Biotium
500uL
p40(Rabbit PAb) Antibody
BNC700375-100
Biotium
100uL
p40(Rabbit PAb) Antibody
BNC700375-500
Biotium
500uL
p40(Rabbit PAb) Antibody
BNC430375-100
Biotium
100uL
p40(Rabbit PAb) Antibody
BNC430375-500
Biotium
500uL
p40(Rabbit PAb) Antibody
BNC400375-100
Biotium
100uL
p40(Rabbit PAb) Antibody
BNC400375-500
Biotium
500uL
p40(Rabbit PAb) Antibody
BNC050375-100
Biotium
100uL
p40(Rabbit PAb) Antibody
BNC050375-500
Biotium
500uL
p40(Rabbit PAb) Antibody
BNCB0375-100
Biotium
100uL
p40(Rabbit PAb) Antibody
BNCB0375-500
Biotium
500uL
p40(Rabbit PAb) Antibody
BNCAP0375-100
Biotium
100uL
p40(Rabbit PAb) Antibody
BNCAP0375-500
Biotium
500uL
p40(Rabbit PAb) Antibody
BNC880375-100
Biotium
100uL
p40(Rabbit PAb) Antibody
BNC880375-500
Biotium
500uL
p40(Rabbit PAb) Antibody
BNCR0375-250
Biotium
250uL
p40(Rabbit PAb) Antibody
BNCP0375-250
Biotium
250uL
p40(Rabbit PAb) Antibody
BNUM0375-50
Biotium
50uL
p40(Rabbit PAb) Antibody
BNCH0375-100
Biotium
100uL
p40(Rabbit PAb) Antibody
BNCH0375-500
Biotium
500uL
p40(Rabbit PAb) Antibody
BNUB0375-100
Biotium
100uL
p40(Rabbit PAb) Antibody
BNUB0375-500
Biotium
500uL
p40(Rabbit PAb) Antibody
BNC040375-100
Biotium
100uL
p40(Rabbit PAb) Antibody
BNC040375-500
Biotium
500uL
p40(Rabbit PAb) Antibody
BNC550375-100
Biotium
100uL
p40(Rabbit PAb) Antibody
BNC550375-500
Biotium
500uL
p40(Rabbit PAb) Antibody
BNC610375-100
Biotium
100uL
p40(Rabbit PAb) Antibody
BNC610375-500
Biotium
500uL
p40(Rabbit PAb) Antibody
BNC680375-100
Biotium
100uL
p40(Rabbit PAb) Antibody
BNC680375-500
Biotium
500uL
p40(Rabbit PAb) Antibody
BNC470375-100
Biotium
100uL
p40(Rabbit PAb) Antibody
BNC470375-500
Biotium
500uL
Rabbit IgG Fab Antibody
R20172-100UG
NSJ Bioreagents
100ug
Rabbit IgG Fab Antibody
R20172BTN-50UG
NSJ Bioreagents
50ug
Rabbit IgG Fab Antibody
R20172SAF-250UG
NSJ Bioreagents
250ug
Rabbit polyclonal antibody preparations are usually made by immunizing rabbits with the antigen to be detected, and then harvesting serum from the animals. The serum is then screened for reactivity with the antigen in a variety of formats including ELISA, Western blotting, and immunohistochemistry. Selected sera are then fractionated and tested for purity. The final product is a concentrated solution of rabbit IgG (immunoglobulin G) that is ready for use in research or diagnostic assays.
Rabbit polyclonal antibodies can be used in a variety of applications, including:
• Western blotting
• Immunohistochemistry (IHC)
• ELISA
• Immunoprecipitation
• In situ hybridization (ISH)
• Flow cytometry
• Chromatin immunoprecipitation (ChIP)
etc.
Today, most monoclonal antibodies are generated in mice or rats, but rabbits are still commonly used to generate polyclonal antibody preparations. The main advantage of using rabbits is that they can be immunized with a wider range of antigens than other animals. Additionally, the cost of producing rabbit polyclonal antibodies is lower than for monoclonal antibodies, making them a more affordable option for many research applications. The disadvantages of using rabbit polyclonal antibodies are that they are less specific than monoclonal antibodies and can cause nonspecific background staining. However, when used with caution, rabbit polyclonal antibodies can be a valuable tool for researchers and clinicians.
What is Rabbit Antibody?
Rabbit polyclonal antibody (immunoglobulin) is produced in an animal that is able to produce antibodies against many antigens. For certain purposes, other sources may be more convenient or even better than rabbits despite possibly shorter supply or greater cost. Rabbits should only be used when the non-specific background staining is not prohibitive; thus care must be taken to avoid nonspecific absorption effects which make it difficult to interpret findings.
The main advantages of using polyclonal are: they are cheap, and they are more likely to bind a wider range of epitopes on the antigen than monoclonal antibodies. The disadvantages are that they are not as specific as monoclonal antibodies and they can cause nonspecific background staining.
Rabbit polyclonal antibody preparations are usually made by immunizing rabbits with the antigen to be detected, and then harvesting serum from the animals. The serum is then screened for reactivity with the antigens in a variety of formats including ELISA, Western blotting, and immunohistochemistry. Selected sera are then fractionated and tested for purity. The final product is a concentrated solution of rabbit IgG (immunoglobulin G) that is ready for use in research or diagnostic assays.
Rabbit polyclonal antibodies can be used in a variety of applications, including:
western blotting, immunohistochemistry (IHC), ELISA, immunoprecipitation, in situ hybridization (ISH), flow cytometry, chromatin immunoprecipitation (ChIP), etc.
Today, most monoclonal antibodies are generated in mice or rats, but rabbits are still commonly used to generate polyclonal antibody preparations. The main advantage of using rabbits is that they can be immunized with a wider range of antigens than other animals. Additionally, the cost of producing rabbit polyclonal antibodies is lower than for monoclonal antibodies, making them a more affordable option for many research applications. The disadvantages of using rabbit polyclonal antibodies are that they are less specific than monoclonal antibodies and can cause nonspecific background staining. However, when used with caution, rabbit polyclonal antibodies can be a valuable tool for researchers and clinicians.
“Rabbit antibody (immunoglobulin) is produced in an animal that is able to produce antibodies against many antigens.” For certain purposes, other sources may be more convenient or even better than rabbits despite possibly shorter supply or greater cost.