Rabbit polyclonal antibody (immunoglobulin) is produced in an animal that is able to produce antibodies against many antigens. Rabbit monoclonal antibody (MAb) has become popular in the past decade, but for most applications rabbit polyclonal antibodies are still used because of their lower cost and wider availability. For certain purposes, other sources may be more convenient or even better than rabbits despite possibly shorter supply or greater cost. Rabbits should only be used when the non-specific background staining is not prohibitive; thus care must be taken to avoid nonspecific absorption effects which make it difficult to interpret findings.
The main advantages of using polyclonal are: they are cheap, and they are more likely to bind a wider range of epitopes on the antigen than monoclonal antibodies. The disadvantages are that they are not as specific as monoclonal antibodies and they can cause nonspecific background staining.
Anti-Dnmt3b Rabbit Monoclonal Antibody Rabbit Monoclonal Antibody
M00319
BosterBio
100ug/vial
Rabbit Albumin Antibody
40150-05011
AssayPro
150 ug
Rabbit Tpa Antibody
abx023929-02mg
Abbexa
0.2 mg
Anti-Rabbit antibody
STJ11100323
St John's Laboratory
100 µl
Rabbit VWCE antibody
orb5385
Biorbyt
200ug
Rabbit Fibrinogen Antibody Pair
55R-1041
Fitzgerald
4 plates
Rabbit Albumin Antibody (HRP)
60-5009
Fitzgerald
1 ml
Rabbit Albumin Monoclonal Antibody
60150-05011
AssayPro
150 ug
GAPDH Rabbit Polyclonal Antibody
A01021-005ml
Abbkine
0.05ml
GAPDH Rabbit Polyclonal Antibody
A01021-02ml
Abbkine
0.2ml
GAPDH Rabbit Polyclonal Antibody
A01021-02ml5
Abbkine
0.2ml×5
Vimentin Rabbit Polyclonal Antibody
38104-100ul
SAB
100ul
Vimentin Rabbit Polyclonal Antibody
38104-50ul
SAB
50ul
LDHD Rabbit Polyclonal Antibody
38105-100ul
SAB
100ul
LDHD Rabbit Polyclonal Antibody
38105-50ul
SAB
50ul
GAPDH Rabbit Polyclonal Antibody
37985-100ul
SAB
100ul
GAPDH Rabbit Polyclonal Antibody
37985-50ul
SAB
50ul
EFHD1 Rabbit Polyclonal Antibody
38001-100ul
SAB
100ul
EFHD1 Rabbit Polyclonal Antibody
38001-50ul
SAB
50ul
Alliinase Rabbit Polyclonal Antibody
38042-100ul
SAB
100ul
Alliinase Rabbit Polyclonal Antibody
38042-50ul
SAB
50ul
ECFP Rabbit Polyclonal Antibody
38077-100ul
SAB
100ul
ECFP Rabbit Polyclonal Antibody
38077-50ul
SAB
50ul
EYFP Rabbit Polyclonal Antibody
38078-100ul
SAB
100ul
EYFP Rabbit Polyclonal Antibody
38078-50ul
SAB
50ul
mOrange Rabbit Polyclonal Antibody
38079-100ul
SAB
100ul
mOrange Rabbit Polyclonal Antibody
38079-50ul
SAB
50ul
mStrawberry Rabbit Polyclonal Antibody
38083-100ul
SAB
100ul
mStrawberry Rabbit Polyclonal Antibody
38083-50ul
SAB
50ul
AmCyan Rabbit Polyclonal Antibody
38086-100ul
SAB
100ul
AmCyan Rabbit Polyclonal Antibody
38086-50ul
SAB
50ul
EBFP Rabbit Polyclonal Antibody
38087-100ul
SAB
100ul
EBFP Rabbit Polyclonal Antibody
38087-50ul
SAB
50ul
Met Rabbit Polyclonal Antibody
ABP57458-003ml
Abbkine
0.03ml
Met Rabbit Polyclonal Antibody
ABP57458-01ml
Abbkine
0.1ml
Met Rabbit Polyclonal Antibody
ABP57458-02ml
Abbkine
0.2ml
VEGF Rabbit Polyclonal Antibody
ABP57460-003ml
Abbkine
0.03ml
VEGF Rabbit Polyclonal Antibody
ABP57460-01ml
Abbkine
0.1ml
VEGF Rabbit Polyclonal Antibody
ABP57460-02ml
Abbkine
0.2ml
CD10 Rabbit Polyclonal Antibody
ABP57461-003ml
Abbkine
0.03ml
CD10 Rabbit Polyclonal Antibody
ABP57461-01ml
Abbkine
0.1ml
CD10 Rabbit Polyclonal Antibody
ABP57461-02ml
Abbkine
0.2ml
NM23A Rabbit Polyclonal Antibody
ABP57462-003ml
Abbkine
0.03ml
NM23A Rabbit Polyclonal Antibody
ABP57462-01ml
Abbkine
0.1ml
NM23A Rabbit Polyclonal Antibody
ABP57462-02ml
Abbkine
0.2ml
ATM Rabbit Polyclonal Antibody
ABP57463-003ml
Abbkine
0.03ml
ATM Rabbit Polyclonal Antibody
ABP57463-01ml
Abbkine
0.1ml
ATM Rabbit Polyclonal Antibody
ABP57463-02ml
Abbkine
0.2ml
ATM Rabbit Polyclonal Antibody
ABP57464-003ml
Abbkine
0.03ml
ATM Rabbit Polyclonal Antibody
ABP57464-01ml
Abbkine
0.1ml
Rabbit polyclonal antibody preparations are usually made by immunizing rabbits with the antigen to be detected, and then harvesting serum from the animals. The serum is then screened for reactivity with the antigen in a variety of formats including ELISA, Western blotting, and immunohistochemistry. Selected sera are then fractionated and tested for purity. The final product is a concentrated solution of rabbit IgG (immunoglobulin G) that is ready for use in research or diagnostic assays.
Rabbit polyclonal antibodies can be used in a variety of applications, including:
• Western blotting
• Immunohistochemistry (IHC)
• ELISA
• Immunoprecipitation
• In situ hybridization (ISH)
• Flow cytometry
• Chromatin immunoprecipitation (ChIP)
etc.
Today, most monoclonal antibodies are generated in mice or rats, but rabbits are still commonly used to generate polyclonal antibody preparations. The main advantage of using rabbits is that they can be immunized with a wider range of antigens than other animals. Additionally, the cost of producing rabbit polyclonal antibodies is lower than for monoclonal antibodies, making them a more affordable option for many research applications. The disadvantages of using rabbit polyclonal antibodies are that they are less specific than monoclonal antibodies and can cause nonspecific background staining. However, when used with caution, rabbit polyclonal antibodies can be a valuable tool for researchers and clinicians.
What is Rabbit Antibody?
Rabbit polyclonal antibody (immunoglobulin) is produced in an animal that is able to produce antibodies against many antigens. For certain purposes, other sources may be more convenient or even better than rabbits despite possibly shorter supply or greater cost. Rabbits should only be used when the non-specific background staining is not prohibitive; thus care must be taken to avoid nonspecific absorption effects which make it difficult to interpret findings.
The main advantages of using polyclonal are: they are cheap, and they are more likely to bind a wider range of epitopes on the antigen than monoclonal antibodies. The disadvantages are that they are not as specific as monoclonal antibodies and they can cause nonspecific background staining.
Rabbit polyclonal antibody preparations are usually made by immunizing rabbits with the antigen to be detected, and then harvesting serum from the animals. The serum is then screened for reactivity with the antigens in a variety of formats including ELISA, Western blotting, and immunohistochemistry. Selected sera are then fractionated and tested for purity. The final product is a concentrated solution of rabbit IgG (immunoglobulin G) that is ready for use in research or diagnostic assays.
Rabbit polyclonal antibodies can be used in a variety of applications, including:
western blotting, immunohistochemistry (IHC), ELISA, immunoprecipitation, in situ hybridization (ISH), flow cytometry, chromatin immunoprecipitation (ChIP), etc.
Today, most monoclonal antibodies are generated in mice or rats, but rabbits are still commonly used to generate polyclonal antibody preparations. The main advantage of using rabbits is that they can be immunized with a wider range of antigens than other animals. Additionally, the cost of producing rabbit polyclonal antibodies is lower than for monoclonal antibodies, making them a more affordable option for many research applications. The disadvantages of using rabbit polyclonal antibodies are that they are less specific than monoclonal antibodies and can cause nonspecific background staining. However, when used with caution, rabbit polyclonal antibodies can be a valuable tool for researchers and clinicians.
“Rabbit antibody (immunoglobulin) is produced in an animal that is able to produce antibodies against many antigens.” For certain purposes, other sources may be more convenient or even better than rabbits despite possibly shorter supply or greater cost.