Rabbit polyclonal antibody (immunoglobulin) is produced in an animal that is able to produce antibodies against many antigens. Rabbit monoclonal antibody (MAb) has become popular in the past decade, but for most applications rabbit polyclonal antibodies are still used because of their lower cost and wider availability. For certain purposes, other sources may be more convenient or even better than rabbits despite possibly shorter supply or greater cost. Rabbits should only be used when the non-specific background staining is not prohibitive; thus care must be taken to avoid nonspecific absorption effects which make it difficult to interpret findings.
The main advantages of using polyclonal are: they are cheap, and they are more likely to bind a wider range of epitopes on the antigen than monoclonal antibodies. The disadvantages are that they are not as specific as monoclonal antibodies and they can cause nonspecific background staining.
Anti-Dnmt3b Rabbit Monoclonal Antibody Rabbit Monoclonal Antibody
M00319
BosterBio
100ug/vial
Rabbit Albumin Antibody
40150-05011
AssayPro
150 ug
Rabbit Tpa Antibody
abx023929-02mg
Abbexa
0.2 mg
Anti-Rabbit antibody
STJ11100323
St John's Laboratory
100 µl
GAPDH Rabbit Polyclonal Antibody
37985-100ul
SAB
100ul
GAPDH Rabbit Polyclonal Antibody
37985-50ul
SAB
50ul
EFHD1 Rabbit Polyclonal Antibody
38001-100ul
SAB
100ul
EFHD1 Rabbit Polyclonal Antibody
38001-50ul
SAB
50ul
Alliinase Rabbit Polyclonal Antibody
38042-100ul
SAB
100ul
Alliinase Rabbit Polyclonal Antibody
38042-50ul
SAB
50ul
ECFP Rabbit Polyclonal Antibody
38077-100ul
SAB
100ul
ECFP Rabbit Polyclonal Antibody
38077-50ul
SAB
50ul
EYFP Rabbit Polyclonal Antibody
38078-100ul
SAB
100ul
EYFP Rabbit Polyclonal Antibody
38078-50ul
SAB
50ul
mOrange Rabbit Polyclonal Antibody
38079-100ul
SAB
100ul
mOrange Rabbit Polyclonal Antibody
38079-50ul
SAB
50ul
mStrawberry Rabbit Polyclonal Antibody
38083-100ul
SAB
100ul
mStrawberry Rabbit Polyclonal Antibody
38083-50ul
SAB
50ul
AmCyan Rabbit Polyclonal Antibody
38086-100ul
SAB
100ul
AmCyan Rabbit Polyclonal Antibody
38086-50ul
SAB
50ul
EBFP Rabbit Polyclonal Antibody
38087-100ul
SAB
100ul
EBFP Rabbit Polyclonal Antibody
38087-50ul
SAB
50ul
Vimentin Rabbit Polyclonal Antibody
38104-100ul
SAB
100ul
Vimentin Rabbit Polyclonal Antibody
38104-50ul
SAB
50ul
LDHD Rabbit Polyclonal Antibody
38105-100ul
SAB
100ul
LDHD Rabbit Polyclonal Antibody
38105-50ul
SAB
50ul
Rabbit Albumin Monoclonal Antibody
60150-05011
AssayPro
150 ug
Rabbit Fibrinogen Antibody Pair
55R-1041
Fitzgerald
4 plates
Rabbit Albumin Antibody (HRP)
60-5009
Fitzgerald
1 ml
GAPDH Rabbit Polyclonal Antibody
A01021-005ml
Abbkine
0.05ml
GAPDH Rabbit Polyclonal Antibody
A01021-02ml
Abbkine
0.2ml
GAPDH Rabbit Polyclonal Antibody
A01021-02ml5
Abbkine
0.2ml×5
Aldolase (Rabbit Muscle) Antibody
abx022821-50mg
Abbexa
50 mg
Rabbit Anti FITC Antibody
E61I01402
EnoGene
100ug
p40(Rabbit PAb) Antibody
BNUB0375-100
Biotium
100uL
p40(Rabbit PAb) Antibody
BNUB0375-500
Biotium
500uL
CD3e(Rabbit PAb) Antibody
BNUB0385-100
Biotium
100uL
CD3e(Rabbit PAb) Antibody
BNUB0385-500
Biotium
500uL
CD8a(Rabbit PAb) Antibody
BNUB0386-100
Biotium
100uL
CD8a(Rabbit PAb) Antibody
BNUB0386-500
Biotium
500uL
TIMP3(Rabbit PAb) Antibody
BNUB0408-100
Biotium
100uL
TIMP3(Rabbit PAb) Antibody
BNUB0408-500
Biotium
500uL
p40(Rabbit PAb) Antibody
BNUM0375-50
Biotium
50uL
CD3e(Rabbit PAb) Antibody
BNUM0385-50
Biotium
50uL
CD8a(Rabbit PAb) Antibody
BNUM0386-50
Biotium
50uL
TIMP3(Rabbit PAb) Antibody
BNUM0408-50
Biotium
50uL
p40(Rabbit PAb) Antibody
BNC040375-100
Biotium
100uL
p40(Rabbit PAb) Antibody
BNC040375-500
Biotium
500uL
CD3e(Rabbit PAb) Antibody
BNC040385-100
Biotium
100uL
CD3e(Rabbit PAb) Antibody
BNC040385-500
Biotium
500uL
Rabbit polyclonal antibody preparations are usually made by immunizing rabbits with the antigen to be detected, and then harvesting serum from the animals. The serum is then screened for reactivity with the antigen in a variety of formats including ELISA, Western blotting, and immunohistochemistry. Selected sera are then fractionated and tested for purity. The final product is a concentrated solution of rabbit IgG (immunoglobulin G) that is ready for use in research or diagnostic assays.
Rabbit polyclonal antibodies can be used in a variety of applications, including:
• Western blotting
• Immunohistochemistry (IHC)
• ELISA
• Immunoprecipitation
• In situ hybridization (ISH)
• Flow cytometry
• Chromatin immunoprecipitation (ChIP)
etc.
Today, most monoclonal antibodies are generated in mice or rats, but rabbits are still commonly used to generate polyclonal antibody preparations. The main advantage of using rabbits is that they can be immunized with a wider range of antigens than other animals. Additionally, the cost of producing rabbit polyclonal antibodies is lower than for monoclonal antibodies, making them a more affordable option for many research applications. The disadvantages of using rabbit polyclonal antibodies are that they are less specific than monoclonal antibodies and can cause nonspecific background staining. However, when used with caution, rabbit polyclonal antibodies can be a valuable tool for researchers and clinicians.
What is Rabbit Antibody?
Rabbit polyclonal antibody (immunoglobulin) is produced in an animal that is able to produce antibodies against many antigens. For certain purposes, other sources may be more convenient or even better than rabbits despite possibly shorter supply or greater cost. Rabbits should only be used when the non-specific background staining is not prohibitive; thus care must be taken to avoid nonspecific absorption effects which make it difficult to interpret findings.
The main advantages of using polyclonal are: they are cheap, and they are more likely to bind a wider range of epitopes on the antigen than monoclonal antibodies. The disadvantages are that they are not as specific as monoclonal antibodies and they can cause nonspecific background staining.
Rabbit polyclonal antibody preparations are usually made by immunizing rabbits with the antigen to be detected, and then harvesting serum from the animals. The serum is then screened for reactivity with the antigens in a variety of formats including ELISA, Western blotting, and immunohistochemistry. Selected sera are then fractionated and tested for purity. The final product is a concentrated solution of rabbit IgG (immunoglobulin G) that is ready for use in research or diagnostic assays.
Rabbit polyclonal antibodies can be used in a variety of applications, including:
western blotting, immunohistochemistry (IHC), ELISA, immunoprecipitation, in situ hybridization (ISH), flow cytometry, chromatin immunoprecipitation (ChIP), etc.
Today, most monoclonal antibodies are generated in mice or rats, but rabbits are still commonly used to generate polyclonal antibody preparations. The main advantage of using rabbits is that they can be immunized with a wider range of antigens than other animals. Additionally, the cost of producing rabbit polyclonal antibodies is lower than for monoclonal antibodies, making them a more affordable option for many research applications. The disadvantages of using rabbit polyclonal antibodies are that they are less specific than monoclonal antibodies and can cause nonspecific background staining. However, when used with caution, rabbit polyclonal antibodies can be a valuable tool for researchers and clinicians.
“Rabbit antibody (immunoglobulin) is produced in an animal that is able to produce antibodies against many antigens.” For certain purposes, other sources may be more convenient or even better than rabbits despite possibly shorter supply or greater cost.